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Öğe Evaluation of (99m)Technetium-Vancomycin Imaging Potential in Experimental Rat Model for the Diagnosis of Infective Endocarditis(Bentham Science Publ Ltd, 2021) Kis, Tuba Tatli; Kose, Sukran; Yilmaz, Osman; Kis, Mehmet; Yurt, Fatma; Acar, Emine; Bekis, RecepBackground: Infective endocarditis (IE) is an infection of the heart's endocardial surface. In recent years, nuclear imaging methods have gained importance in the diagnosis of IE. The present study aims to investigate the imaging potential of Tc-99m-labeled vancomycin (Tc-99m-Van-comycin) as a new agent that would enable the diagnosis of IE in its early stages when it is difficult to diagnose or has small vegetation in the experimental rat model. Methods: Tc-99m-Vancomycin scintigraphy was evaluated for its accumulation in IE with Staphylococcus aureus performed in an experimental rat model. Serial planar scintigraphic and biodistribution analysis of infected vegetations are compared to rats with sterile vegetations. The heart was identified as an infected organ, the liver was identified as a non-infected organ and the heart/liver uptake ratio (T / NT ratio) was compared between infective endocarditis and sterile endocarditis groups. Results: Planar scintigrams (in vivo measurements) showed more uptake in the heart of rats in the infective endocarditis group compared to the uptake in the heart of rats in the sterile endocarditis group, but this difference was not statistically significant (p>0.05). From the ex vivo measurements, the Tc-99m-Vancomycin heart uptake increased significantly (p = 0.016), liver uptake was significantly decreased (p = 0.045) and the T/NT ratio was significantly higher (p = 0.014) in the infective endocarditis group compared to the sterile endocarditis group. Conclusion: In this experimental study, Tc-99m-Vancomycin scintigraphy ensured the detection of ex vivo infected tissue in a rat model of IE. In addition, the absence of significant Tc-99m-Van-comycin uptake in the sterile endocarditis group indicates that this agent targeted the infected tissue instead of the sterile inflammatory tissue. Finally, this agent should also be evaluated with animal-specific imaging devices.Öğe AN EXPERIMENTAL INFECTIVE ENDOCARDITIS MODEL IN RATS(Dokuz Eylul Univ Inst Health Sciences, 2021) Kose, Sukran; Kis, Tuba Tatli; Yilmaz, Canberk; Kis, Mehmet; Baris, Mustafa; Diniz, Gulden; Yilmaz, OsmanObjective: Infective endocarditis (IE) is defined as infection of the endocardial surface of the heart. Updates are needed in the diagnosis and treatment of IE, as well as in animal models of IE. Based on this need, a new model of infective endocarditis induced by S. aureus was described in our study. Methods: This study was performed on 7 Wistar albino male rats, each aged six months and weighing 250-300 g. Underwent the surgical implantation of a 20 G catheter, to gain access to right common carotid artery. Twenty-four hours after implantation, 0.5 ml 100.000 colony forming unit (cfu) of S. aureus was injected via the tail vein and 3 days later echocardiography was performed and rats subsequently sacrificed. IE was later diagnosed histopathologically. Results: Two of the rats were exitus one day after S. auerus was given. The mortality rate of the experiment was 28.5%. Histopathological examination revealed vegetations and bacterial colonization were detected in the endocardium in all rats that protruded from the endocardium to the cardiac cavity. Conclusion: Our study is the first study in the literature to identify the IE rat model using the 20 G catheter. Due to the practical application of the surgical procedure (use of 20 G catheter) in our study, we think that it will provide much convenience to the researchers in the experimental research on IE diagnosis and treatment.Öğe A New Experimental Allergic Rhinitis Model in Mice(Galenos Publ House, 2021) Kose, Sukran; Kis, Tuba Tatli; Diniz, Gulden; Akbulut, Ilkay; Serin, Basak Gol; Yilmaz, Canberk; Ozyazici, MineObjective: Allergic rhinitis (AR) is an inflammatory disease of the nasal mucosa mediated by IgE after exposure to an allergen. The most well known related comorbidity of AR is asthma. This study was planned due to the need for an animal model for studies on AR-asthma coexistence. In this study, the frequency of AR accompanying in the asthma model created in mice,and the usability of the related model in AR studies will be investigated. Methods: In our study, 6-8 week-old, 18-20 g BALB/c mice were used. Chicken egg ovalbumin (OVA Grade V, Sigma) was administered through intraperitoneal (IP) route at doses of 10 mu g on days 0 and 14. Mice were exposed to aerosolized 2.5% ovalbumin solution in sterile saline for 30 minutes 3 days a week for 8 weeks, starting 7 days after the last IP administration (21st day). After exposure to OVA, mice were observed for typical signs of AR including sneezing, runny nose, and nasal itching. The final diagnosis of AR was made by histopathological examination of the rhinotracheal tissues of mice. Results: In our study, all mice exposed to ovalbumin received histopathologic diagnosis of AR. Increased number of capillaries lymphocytes, polymorphonuclear leukocytes and eosinophilsper square millimetre of rhinotracheal tissues were calculated in the murine model of AR compared to the the control group. Conclusion: This study introduced a new AR model, not cited in the literature, and induced with the longest-term ovalbumin exposure in the literature. It was concluded that this model, known as the asthma model, can also be used to induce an AR model and can be used in studies investigating coexistence of allergic rhinitis and asthma.Öğe A new experimental allergic rhinitis model in mice [Meeting Abstract](Wiley, 2021) Kose, Sukran; Kis, Tuba Tatli; Diniz, Gulden; Akbulut, Ilkay; Serin, Basak Gol; Yilmaz, Canberk; Ozyazici, Mine[No Abstract Available]
