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Öğe Bio-based fractions by hydrothermal treatment of olive pomace: Process optimization and evaluation(Elsevier Ltd, 2015) Kazan A.; Celiktas M.S.; Sargin S.; Yesil-Celiktas O.Olive pomace is an important lignocellulosic biomass for Mediterranean countries which is released in large quantities during industrial olive oil production and used for heating purposes for many years. In this study, the aim was to investigate the use of olive pomace to obtain value added fractions namely, proteins, fermentable sugars and lignin by sustainable biorefinery approach applying a sequence of high pressure extraction and hydrolysis. After pretreatment steps, 93.7% of the fermentable sugars and 94.4% of the lignin that present in the biomass were recovered. Liquid hot water (LHW) pretreatment was shown to enhance the yield of enzymatic hydrolysis by an increase of 95.23%. The obtained sugars were used to produce bioethanol and based on the consumed sugar the yield and productivity were determined as 15.25% and 0.086 kg/m3 h respectively. © 2015 Elsevier Ltd. All rights reserved.Öğe Formulation of organic and inorganic hydrogel matrices for immobilization of ß-glucosidase in microfluidic platform(Wiley-VCH Verlag, 2017) Kazan A.; Heymuth M.; Karabulut D.; Akay S.; Yildiz-Ozturk E.; Onbas R.; Muderrisoglu C.; Sargin S.; Heils R.; Smirnova I.; Yesil-Celiktas O.The aim of this study was to formulate silica and alginate hydrogels for immobilization of ß-glucosidase. For this purpose, enzyme kinetics in hydrogels were determined, activity of immobilized enzymes was compared with that of free enzyme, and structures of silica and alginate hydrogels were characterized in terms of surface area and pore size. The addition of polyethylene oxide improved the mechanical strength of the silica gels and 68% of the initial activity of the enzyme was preserved after immobilizing into tetraethyl orthosilicate–polyethylene oxide matrix where the relative activity in alginate beads was 87%. The immobilized ß-glucosidase was loaded into glass–silicon–glass microreactors and catalysis of 4-nitrophenyl ß-d-glucopyranoside was carried out at various retention times (5, 10, and 15 min) to compare the performance of silica and alginate hydrogels as immobilization matrices. The results indicated that alginate hydrogels exhibited slightly better properties than silica, which can be utilized for biocatalysis in microfluidic platforms. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim