Yazar "Karakus, M." seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Molecular screening of Leishmania spp. infection and bloodmeals in sandflies from a leishmaniasis focus in southwestern Turkey
    (Wiley, 2017) Karakus, M.; Pekagirbas, M.; Demir, S.; Eren, H.; Toz, S.; Ozbel, Y.
    Leishmaniasis is an arthropod-borne disease that affects approximately 2 million people worldwide annually. The aims of this study were to detect the presence of Leishmania (Kinetoplastida: Trypanosomatidae) DNA and the feeding preferences of probable vector species in an endemic focus of Leishmania infantum in Turkey. Entomological sampling was performed in August and October 2015 in Aydn province, where cases of human and canine leishmaniasis have been reported previously. A total of 1059 sandfly specimens comprising nine species belonging to two genera, Phlebotomus and Sergentomyia (both: Diptera: Psychodidae), and five subgenera of the Phlebotomus genus (Phlebotomus, Paraphlebotomus, Larroussius, Adlerius and Transphlebotomus) were collected in five villages. Among all Phlebotomus specimens, Phlebotomus neglectus (39%) was noted as the most abundant species, followed by Phlebotomus tobbi (18%). LeishmaniaDNA was detected in pools from P.neglectus, P.tobbi and Sergentomyia dentata by kDNA polymerase chain reaction (PCR). LeishmaniaDNA from Phlebotomus specimens was identified as L.infantum, but LeishmaniaDNA from Sergentomyia spp. could not be identified to species level by ITS-1 real-time PCR. The detection of LeishmaniaDNA in wild-caught P.neglectus and the high percentage (24.2%) of human DNA in engorged specimens suggests that P.neglectus is probably an important vector species for L.infantum in Aydn province.
  • Küçük Resim Yok
    Öğe
    sandflyDST: a dynamic web-based decision support tool for the morphological identification of sandflies present in Anatolia and mainland Europe, and user study
    (Wiley, 2016) Karakulah, G.; Karakus, M.; Suner, A.; Demir, S.; Arserim, S. K.; Toz, S.; Ozbel, Y.
    Species identification of sandflies is mainly performed according to morphological characters using classical written identification keys. This study introduces a new web-based decision support tool (sandflyDST) for guiding the morphological identification of sandfly species present in Anatolia and mainland Europe and classified in the Phlebotomus and Sergentomyia genera (both: Diptera: Psychodidae). The current version of the tool consists of 111 questions and 36 drawings obtained from classical written keys, and 107 photographs for the quick and easy identification of 26 species of the genus Phlebotomus and four species of the genus Sergentomyia. The tool guides users through a decision tree using yes/no questions about the morphological characters of the specimen. The tool was applied by 30 individuals, who then completed study questionnaires. The results of subsequent analyses indicated that the usability ((x) over bar (SUS Score) = 75.4) and users' level of appreciation (86.6%) of the tool were quite high; almost all of the participants considered recommending the tool to others. The tool may also be useful in training new entomologists and maintaining their level of expertise. This is a dynamic tool and can be improved or upgraded according to feedback. The tool is now available online at http://parasitology.ege.edu.tr/sandflyDST/index.php.