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    Prediction, identification and evaluation of bioactive peptides from tomato seed proteins using in silico approach
    (Springer, 2020) Kartal, Canan; Kaplan Turkoz, Burcu; Otles, Semih
    The identification and isolation of bioactive peptides from food sources is a very active research area considering their potential use in both functional food products and pharmaceuticals. in silico tools can be used to predict possible bioactivity of peptides and can aid experimental procedures by narrowing down high number of enzyme combinations for enzymatic hydrolysis of proteins. in this study, tomato seed, which is a valuable industrial waste, was investigated as a potential bioactive peptide source. Nine tomato (Solanum lycopersicum) seed proteins were chosen for in silico analysis to assess their potential ACE inhibitory, DPP-IV inhibitory and antioxidative peptides. After homology assessment, the profiles of potential biological activity of proteins and the simulated enzymatic hydrolysis with fifteen individual enzymes and two enzyme combinations were performed and the released peptides were evaluated. This study demonstrates that tomato seed proteins represent a remarkable source for the generation of bioactive peptides. According to the in silico results, it is predicted that enzymatic hydrolysis with pepsin and pepsin + trypsin combination may produce peptide fragments with high bioactivity. However, it should be noted that the resulting peptides and their activities were predicted under in silico conditions that might be different from experimental conditions.
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    Structural and molecular characterization of collagen-type I extracted from lamb feet
    (Wiley, 2023) Ata, Özge; Bakar, Bahar; Kaplan Turkoz, Burcu; Kumcuoğlu, Seher; Aydoğdu, Yıldırım; Gümüştaş, Barış; Dinler Doğanay, Gizem
    This study aimed to extract collagen-I from lamb feet (LF) and examine the effects of ultrasound treatment on the structural and molecular characteristics of the collagen. Compared to ultrasonic bath treatment and conventional extraction methods, ultrasonic probe (USP) treatment significantly increased the collagen content of the extract (p < 0.05). The electrophoretic profiles confirmed the presence of alpha- and beta-chains, indicating it as type I. Furthermore, X-ray diffraction, Fourier-transform infrared spectroscopy, and circular dichroism spectra analyses revealed that the extraction method did not adversely affect the triple helix structure of the collagen. Moreover, the fibrillar structure of the collagen samples was verified through scanning electron microscopy analyses. Notably, the LF collagen exhibited a high thermal denaturation temperature owing to its elevated imino acid content. The collagen samples exhibited high solubility in acidic pH but low solubility in high salt concentrations. The present findings signified that sonication with USP can effectively enhance the yield of collagen from LF without compromising its quality.
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    UV-crosslinked collagen-chondroitin sulfate nanofibers: Insights on production, characterization, in-vitro digestibility
    (Elsevier Sci Ltd, 2024) Ata, Ozge; Bakar, Bahar; Kaplan Turkoz, Burcu; Kumcuoglu, Seher; Kazanci, Murat; Tavman, Sebnem
    Electrospun polymer nanofibers have significant attention in various fields. In this study, collagen/chondroitin sulfate (Col/CS) nanofibers were fabricated by electrospinning, the effect of solution and processing parameters on fiber morphology was investigated. The resulting fibers were exposed to UV light for the crosslinking process. Granola bars with Col/CS nanofibers were prepared and subjected to in vitro digestion. The electrospun Col/CS nanofibers, fabricated at a 95:5 (w/w) ratio under 26 kV voltage, 0.8 mL/h feeding rate, and a 15 cm tip-to-collector distance, exhibited a smooth, bead-free fibrous morphology with an average diameter of 79.88 +/- 6.01 nm. After UV-crosslinking, no new bands were observed in the FTIR spectrum, but notable changes occurred in the Amide I and II regions. Granola bars containing Col/CS nanofibers exhibited significantly higher Col content in the intestinal digestion fraction of in vitro digestion compared to the control group (free Col + CS). On the other hand, no significant difference was observed between the CS content of the samples after intestinal digestion. These findings demonstrate that electrospinning followed by UV crosslinking is an effective method for producing Col/CS fibers without the use of harsh solvents, highlighting Col/CS nanofibers as promising candidates for the development of novel functional foods.