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Öğe Effect of Cutting Treatment on Seed Yield and Seed Quality of Dill(Univ Namik Kemal, 2023) Alan, Ozlem; Ilbi, HulyaDill, Anethum graveolens L., is grown in various regions of the world. It has been used as vegetable and medicinal plant since ancient times. Seed quality is important in dill cultivation and there is limited information on the effects of cutting treatment in this species. This research was carried out to find out the effect of cutting treatment on phenological traits, seed yield and quality of dill at Ege University, Izmir, during 2016-2018. The experiments were carried out in the randomized complete block design with three replications, comprising of cutting treatment for both spring and autumn seeding period. Significant differences were noted in the flowering period, seed yield and quality attributes with cutting treatment for both seeding period. The obtained results showed that the days from sowing to bolting initiation were increased with cutting treatment from 51 days to 65 days in spring seeding period and from 153 days to 184 days in autumn seeding period. The plant height, number of umbels per plant, seed weight per plant were lower in the cut plants in comparison to that of uncut plants for both seeding period. The uncut plants has produced significantly higher plant height (113 cm-spring seeding period; 133 cm-autumn seeding period), number of umbels/plant (8.4-spring seeding period; 10.1-autumn seeding period) and seed weight/plant (6.4 g-spring seeding period; 10.9 g-autumn seeding period). The cutting treatment decreased germination percentage by 9.27% in the spring seeding period and by 12.13% in the autumn seeding period compared to the control plant seeds. Lower mean germination time at 20/30 degrees C (3.77 days-spring seeding period; 4.54 days-autumn seeding period) were also observed in uncut plants. Thus, it was concluded that cutting is not recommended in the vegetative stage in dill seed production as it provided lower seed yield and seed quality for both autumn and spring seed planting periods.Öğe Evaluation of phenotypic diversity and geographical variation of cultivated (Eruca sativa L.) and wild (Diplotaxis tenuifolia L.) rocket plant(Cambridge Univ Press, 2011) Bozokalfa, M. Kadri; Esiyok, Dursun; Ilbi, Hulya; Kavak, Suleyman; Asciogul, Tansel KaygisizRocket (Eruca spp. and Diplotaxis spp.) is widely cultivated as a green vegetable in Mediterranean countries, Europe, United States and Brazil, but few rocket cultivars are available and limited variety selection has taken place. Plant genetic resources and germplasm are fundamental sources for plant breeding, and the assessment of the genetic diversity among germplasm accessions is useful to facilitate the more efficient use of plant genetic resources. The objective of this study was to characterize phenotypic diversity and relationships among genotypes of Eruca and Diplotaxis originating from 11 countries across three regions (Europe, Africa and Asia). Principal component analyses explained over 85% of total variation for 51 qualitative and quantitative agromorphological traits. Cluster analyses divided the accessions into five major clusters according to plant traits, siliqua properties and geographical origin of genotype with a few exceptions. These findings indicate a number of useful traits in the gene pool and a wide range of phenotypic variation that provides a good source of diversity for use in modern Eruca breeding programmes.Öğe Genetic Diversity of some Daffodil (Narcissus L. spp.) Genotypes from Turkey by Using SRAP Markers(Univ Agr Sci & Veterinary Med Cluj-Napoca, 2019) Zeybekoglu, Emrah; Kaygisiz Asciogul, Tansal; Ilbi, Hulya; Ozzambak, Mustafa E.Daffodil (Narcissus L. spp.) is one of the oldest ornamental flowers that have been cultivated from ancient times to nowadays. the species Narcissus tazetta L. and N. serotinus L. have spread out in different regions of Turkey and some other species have been naturalized. the purpose of this study was to characterize some daffodil populations that were collected from different regions of Turkey by using SRAP markers. Twenty-two daffodil populations of different species such as N. tazetta, N. serotinus, N. pseudonarcissus L., N. poeticus L. and N. jonquilla L. were used. Thirty-seven of 48 SRAP primer combinations showed scorable and polymorphic bands between samples. the similarity coefficients were used to construct a dendrogram by Unweighted Pair-Group Method with Arithmetic Averages (UPGMA) using NTSYS-PC. Cophenetic correlation between ultrametric similarities of tree and similarity matrix were high (r = 0.97). the genotypes of Narcissus species were divided into 2 main groups. Although there was less genetic variation among N. tazetta species, even collected from different regions of Turkey, N. poeticus, N. pseudonarsis, N. jonquilla and N. serotinus had more genetic divergence among them. It can be concluded that genetic variation of Narcissus species can be used for daffodil breeding programs.Öğe Genetic mapping and inheritance of Russian wheat aphid resistance gene in accession IG 100695(Wiley, 2016) Tonk, Fatma Aykut; Istipliler, Deniz; Tosun, Muzaffer; Turanli, Ferit; Ilbi, Hulya; Cakir, MehmetThe Russian wheat aphid (RWA), Diuraphis noxia (Kurdjumov), is an important pest of small-grain cereals, particularly wheat, worldwide. The most efficient strategy against the RWA is to identify sources of resistance and to introduce them into susceptible wheat genotypes. This study was conducted to determine the mode of inheritance of the RWA resistance found in ICARDA accession IG 100695, to identify wheat microsatellite markers closely linked to the gene and to map the chromosomal location of the gene. Simple sequence repeat (SSR) marker scores were identified in a mapping population of 190F(2) individuals and compared, while phenotypic screening for resistance was performed in F-2:3 families derived from a cross between Basribey' (susceptible) and IG 100695 (resistant). Phenotypic segregation of leaf chlorosis and rolling displayed the effect of a single dominant gene, temporarily denoted Dn100695, in IG 100695. Dn100695 was mapped on the short arm of chromosome 7D with four linked SSR markers, Xgwm44, Xcfd14, Xcfd46 and Xbarc126. Dn100695 and linked SSR markers may be useful for improving resistance for RWA in wheat breeding.Öğe Genetic variability for mineral concentration of Eruca sativa L. and Diplotaxis tenuifolia L. accessions(Brazilian Soc Plant Breeding, 2009) Bozokalfa, Mehmet Kadri; Yagmur, Bulent; Ilbi, Hulya; Esiyok, Dursun; Kavak, SuleymanEruca sativa L. (rocket or arugula) and Diplotaxis tenuifolia L. (perennial wall-rocket), are important leafy vegetables and are significant sources of minerals for human nutrition and commonly found in the Mediterranean basin, southern Europe, and Central Asia The objectives of this study were to determine genotypic variability among and within E sativa and D tenuifolia genotypes for NO(3,) NO(2,) N, P, K, Ca, Mg, Na, Fe, Cu, Zn, and Mn concentrations, to estimate genotype environment interaction, and to assess relationships among leaf mineral concentration during two consecutive spring seasons E sativa and D tenuifolia leaves contained significant amounts of nutritionally important minerals In general, genotype variations was lower than phenotype variation for all mineral concentrations considered, indicating the influence of environment on the expression of analyzed traits The variance between genotypes and relative importance within genotype variation indicates that NO(3), NO(2), K, Mn, Zn, and Cu concentrations may be improved by selecting among cultivars, if the heritability is adequateÖğe Genetic variability for mineral concentration of Eruca sativa L. and Diplotaxis tenuifolia L. accessions(Brazilian Soc Plant Breeding, 2009) Bozokalfa, Mehmet Kadri; Yagmur, Bulent; Ilbi, Hulya; Esiyok, Dursun; Kavak, SuleymanEruca sativa L. (rocket or arugula) and Diplotaxis tenuifolia L. (perennial wall-rocket), are important leafy vegetables and are significant sources of minerals for human nutrition and commonly found in the Mediterranean basin, southern Europe, and Central Asia The objectives of this study were to determine genotypic variability among and within E sativa and D tenuifolia genotypes for NO(3,) NO(2,) N, P, K, Ca, Mg, Na, Fe, Cu, Zn, and Mn concentrations, to estimate genotype environment interaction, and to assess relationships among leaf mineral concentration during two consecutive spring seasons E sativa and D tenuifolia leaves contained significant amounts of nutritionally important minerals In general, genotype variations was lower than phenotype variation for all mineral concentrations considered, indicating the influence of environment on the expression of analyzed traits The variance between genotypes and relative importance within genotype variation indicates that NO(3), NO(2), K, Mn, Zn, and Cu concentrations may be improved by selecting among cultivars, if the heritability is adequateÖğe Growing season climate affects phenological development, seed yield and seed quality of dill (Anethum graveolens)(Ista-Int Seed Testing Assoc, 2022) Alan, Ozlem; Kanturer, Damla; Powell, Alison A.; Ilbi, HulyaDill seed production was investigated over two seasons, comprising a spring growing cycle (SGC) and an autumn growing cycle (AGC). The effects of growing cycle on phenological traits, yield and quality of dill seeds formed on different umbels of the mother plant were investigated. Significant differences were noted in the flowering period, seed yield and quality parameters. The SGC resulted in a shorter time from sowing to bolting and flowering initiation compared with the AGC. Plant height, number of umbels/plant, number of umbelets/ umbel, umbel diameter, umbel length and seed weight/plant increased in the AGC. In contrast, decreased germination at 20/30??C and at 13??C, and increased mean germination time at 20/30??C in AGC indicated lower seed quality compared with SGC. Primary umbels produced the best yield and higher quality seeds, followed by the secondary umbels while tertiary umbels gave poor yield and quality seeds in both SGC and AGC. In conclusion, AGC was advisable for higher seed yield, but SGC resulted in higher seed quality compared with the AGC. This highlights the need to select a suitable growing cycle to guarantee high seed yield and quality for each seed production environment.Öğe INVESTIGATION ON IDENTIFICATION OF NEW RESISTANT RESOURCES TO BACTERIAL CANKER AND WILT DISEASE(Parlar Scientific Publications (P S P), 2018) Kabas, Aylin; Boyaci, H. Filiz; Horuz, Sumer; Aysan, Yesim; Ilbi, HulyaClavibacter michiganensis subsp. michiganensis (Smith) Davis et al (Cmm) is an important bacterial agent for tomatoes causing destructive disease. The best way to control of this disease is to use resistant cultivars. Unfortunately there has not been developed a resistant variety against the Cmm because the heritability of resistance is quite complex. In this study, the reactions of 78 genotypes including wild accessions of tomato and cultivar types were investigated. Cmm suspension adjusted to 10(7)cfu/ml concentration was injected in the wound for the inoculation. Seedlings were incubated in a climate controlled greenhouse. Disease symptoms were scored seven times during eight weeks by using the 0-4 scale values from inoculation. Three genotypes were showed resistance against the Cmm. S. peruvianum and S. chmielewskii were found as tolerant and S. habrochaites was determined as moderate tolerant. Both of genotypes have great importance as new resistance sources that they don't need embryo rescue like S. peruvianum.Öğe Optimization of Protocol for Construction of Fungal ITS Amplicon Library for High-Throughput Illumina Sequencing to Study the Mycobiome of Aspen Leaves(Mdpi, 2022) Albrectsen, Benedicte Riber; Albrectsen, Benedicte Riber; Ilbi, Hulya; Siddique, Abu BakarHigh-Throughput Illumina Sequencing (HTS) can be used to study metagenomes, for example, those of importance for plant health. However, protocols must be optimized according to the plant system in question, the focal microorganisms in the samples, the marker genes selected, and the number of environmental samples. We optimized the protocol for metagenomic studies of aspen leaves, originating from varied genotypes sampled across the growing season, and consequently varying in phenolic composition and in the abundance of endo- and epiphytic fungal species. We optimized the DNA extraction protocol by comparing commercial kits and evaluating five fungal ribosomal specific primers (Ps) alone, and with extended primers that allow binding to sample-specific index primers, and we then optimized the amplification with these composite Ps for 380 samples. The fungal DNA concentration in the samples varied from 561 ng/mu L to 1526 ng/mu L depending on the DNA extraction kit used. However, binding to phenolic compounds affected DNA quality as assessed by Nanodrop measurements (0.63-2.04 and 0.26-2.00 absorbance ratios for 260/280 and 260/230, respectively), and this was judged to be more important in making our choice of DNA extraction kit. We initially modified the PCR conditions after determining the concentration of DNA extract in a few subsamples and then evaluated and optimized the annealing temperature, duration, and number of cycles to obtain the required amplification and PCR product bands. For three specific Ps, the extended Ps produced dimers and unexpected amplicon fragments due to nonspecific binding. However, we found that the specific Ps that targeted the ITS2 region of fungal rDNA successfully amplified this region for every sample (with and without the extension PP) resulting in the desired PCR bands, and also allowing the addition of sample-specific index primers, findings which were successfully verified in a second PCR. The optimized protocol allowed us to successfully prepare an amplicon library in order to subject the intended 380 environmental samples to HTS.Öğe Relationship between some morphological traits of the tomato lines and resistance to tomato yellow leaf curl virus disease(Univ Agr Sci & Veterinary Med Cluj-Napoca, 2020) Zengin, Sinan; Kabas, Aylin; Ilbi, HulyaTomato (Solanum lycopersicum L.) is the most produced and exported vegetable in Turkey. There are many pathogens to limit tomato production by reducing yield and fruit quality. Among them, Tomato Yellow Leaf Curl Virus (TYLCV) causes important economic losses. the most efficient and environmentally friendly method against TYLCV is the use of resistant varieties. in this trial, it was aimed to determine some morphological traits which were linked to Ty-1 and Ty-3a genes which confer to TYLCV. A commercial hybrid carrying Ty-1, Ty-3a genes as heterozygous was crossed to a susceptible inbred line from Bati Akdeniz Agricultural and Research Institute (BATEM). Marker assisted selection (MAS) was carried out in F1 and F2 generations and biological tests were done for TYLCV resistance in F3 generation. MAS for Ty-3a and Ty-1 genes were compatible with biological tests. in total of 95 genotypes in F3 were developed with molecular marker selection. It was determined that 30 genotypes having the Ty-3a and Ty-1 genes as homozygous resistant. the nine genotypes carried these genes in heterozygous form. 56 genotypes were identified as susceptible. the 43 morphological traits were observed in identified individuals to correlate with resistant allele, Ty-3a. It was found that there was statistically important correlation between Ty-3a and length of internode, length of stem at first inflorescence, status of calix, leaf attitude, length of inflorescence and plant habitus. Also, there was negative correlation between fruit weight, fruit length and resistance. Therefore, we identified some morphological markers linked to Ty-3a which can be used in selection for TLYCV resistant breeding programme.Öğe Single radicle emergence count for predicting vigour of marigold (Tagetes spp.) seed lots(Ista-Int Seed Testing Assoc, 2020) Ilbi, Hulya; Powell, Alison A.; Alan, OzlemThis study was carried out to determine whether a single radicle emergence count (RE) during germination can be used for vigour assessment to estimate field emergence of marigold (Tagetes spp.) seed lots. Six marigold seed lots (Tagetes erecta and T. patula) with normal germination above 75% were germinated using the standard ISTA germination test and radicle emergence (production of 2 mm radicle) counted at regular intervals from 25 to 169 hours. The seed lots were also sown in the field with final seedling emergence assessed after 25 days. Seedling emergence was highly correlated with the radicle emergence count after 49 and 66 hours germination (r = 0.90 and r = 0.91, respectively; P < 0.01) but not with germination percentage (r = 0.26). Thus, the radicle emergence counts at 49 and 66 hours accounted for 81 or 83% of the variation in field emergence, respectively. The results indicate that a 49- or 66-hour count of RE during germination could be used as a vigour test to estimate field emergence for marigold seed lots.
