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    Enzymatic synthesis of I-125/131 labeled 8-hydroxyquinoline glucuronide and in vitro/in vivo evaluation of biological influence
    (Pergamon-Elsevier Science Ltd, 2011) Yesilagac, Reyhan; Unak, Perihan; Medine, E. Ilker; Ichedef, Cigdem A.; Ertay, Turkan; Muftuler, F. Z. Biber
    8-Hydroxyquinoline (8-OHQ) is a long-known molecule which due to its metal-complexation ability is frequently used for analysis. It is also called oxine. Oxine and derivatives have been investigated to process antitumor and antimicrobial activities. 8-Hydroxyquinolyl-glucuronide (8-OHQ-Glu) was enzymatically synthesized using microsome preparates separated from Hutu-80 cells, labeled with I-125 to perform a radionuclide labeled prodrug and investigated of its biological affinities on Hutu-80 (human duodenum intestinal adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), Detroit 562 (human pharynx adenocarcinoma) cells and ACBRI 519 (primary human small intestine epithelial cells) in this work. UDP-glucuronyl transferase (UDPGT) rich microsome preparates, which are used for glucuronidation in enzymatic synthesis, were extracted from Hutu-80 cells. 8-OHQ-Glu components were labeled using iodogen method with I-125 and I-131. Structural analyses were performed with LC/MS/MS, H-1 NMR and C-13-MMR for identify and measure chemical constituents. Results confirmed expected molecular structure. 8-OHQ-Glu could successfully radioiodinated with I-125/131 according to iodogen method. I-125-8-OHQ-glucuronide incorporated with human gastrointestinal cancer cells such as Detroit-562 (human pharynx adenocarcinoma) (12.6%), Caco-2 (human colorectal adenocarcinoma) (7.8%), Hutu-80 (human duodenum intestinal adenocarcinoma) (9.5%) and ACBRI 519 (primary human small intestine epithelial cells) (6.40%). I-131-8-OHQ-Glu was tested in mice bearing subcutaneously implanted Caco-2 colorectal adenocarcinoma cells. The results demonstrated that radioiodinated 8-OHQ-Glu may be promising anticancer prodrug. (C) 2010 Elsevier Ltd. All rights reserved.
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    Metabolic Comparison of Radiolabeled Bleomycin and Bleomycin-Glucuronide Labeled with Tc-99m
    (Mary Ann Liebert Inc, 2011) Kocan, Feray; Avcibasi, Ugur; Unak, Perihan; Muftuler, Fazilet Zumrut Biber; Ichedef, Cigdem A.; Demiroglu, Hasan; Gumuser, Fikriye G.
    The metabolic comparison of bleomycin (BLM) and bleomycin-glucuronide (BLMG) radiolabeled with Tc-99m (Tc-99m-BLM and Tc-99m-BLMG, respectively) has been investigated in this study. Quality control procedures were carried out using thin-layer radiochromatography and high-performance liquid chromatography. To compare the metabolic behavior of BLM and its glucuronide conjugate radiolabeled with Tc-99m, scintigraphic, and biodistributional techniques were applied using male New Zealand rabbits and Albino Wistar rats. The results obtained have shown that these compounds were successfully radiolabeled with a labeling yield of about 100%. Maximum uptakes of Tc-99m-BLM and Tc-99m-BLMG metabolized as N-glucuronide were observed within 2 hours in the liver, the bladder, and the spinal cord for Tc-99m-BLM and the lung, the liver, the kidney, the large intestine, and the spinal cord for Tc-99m-BLMG, respectively. Scintigraphy and biodistributional studies performed on the experimental animals have shown that radiopharmaceutical potentials of these compounds are completely different. At the same time, uptake of the Tc-99m-BLMG was found to be better than that of Tc-99m-BLM.