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Öğe Analysis of expressed genes in normal and tumoral mammary gland tissue of the terrier dog [Terrier köpeklerinin normal ve tümörlü meme bezi dokusunda eksprese edilen genlerin birincil analizi](Veteriner Fakultesi Dergisi, 2018) Ozdemir Ozgenturk N.; Omeroglu Ulu Z.; Ulu S.; Celik M.; Tellioglu B.; Yildirim F.; Akis Akad I.; Gurel A.; Un C.; Oztabak K.O.Mammary gland tumor is the most common type of tumor in female dogs. Data on genes that are involved in tumorigenesis and mechanism of tumor development are insufficient. Comparative studies have been conducted in order to see if tumorigenesis studies in the dog could be a model for human mammary gland tumors. In this study, we constructed two different cDNA libraries from mammary tissue, which were collected from a normal mammary tissue of a healthy Terrier dog and a tumoral mammary tissue of a sick dog. A total 2304 colonies which are randomly picked out from the two libraries were sequenced for developing a dog mammary gland ESTs collection. Raw EST data were analyzed with Phred/Phrap programs and readable EST sequences were assembled with the CAP3 program. All of EST sequences were grouped into 45 contig and 2203 singletons. Putative functions of all unique sequences were designated by NCBI BLAST based on gene homology and annotated by BLAST2GO. The results of this study are a very valuable resource for functional genome studies of the dogs. © 2018, Veteriner Fakultesi Dergisi. All rights reserved.Öğe Effect of reddening of cotton (Gossypium hirsutum L.) leaves on the ultrastructure of mesophyll cells(2005) Stoyanova-Koleva D.; Edreva A.; Velikova V.; Gurel A.The ultrastructure of cotton leaves, exhibiting reddening as symptom of physiological disorder, was examined by means of transmission electron microscopy. Osmiophilisation of the membrane compartment was established. Massive agglomerations on the tonoplast in the vacuole of cells under the adaxial epidermis were observed, and were referred to as electron-dense osmiophilic substance, most probably of anthocyanin nature. In chloroplast stroma a zone of low electron density enclosing numerous osmiophilic aggregations of unclear chemical character was differentiated. Fragmentation and severe destruction of thylakoids in chloroplasts of reddening cotton leaves was not detected.Öğe Micropropagation of centennial tertiary relict trees of Liquidambar orientalis Miller through meristematic nodules produced by cultures of primordial shoots(Springer Verlag, 2015) Bayraktar M.; Hayta S.; Parlak S.; Gurel A.Key message: This paper provides an important micropropagation method that might be used for conservation and commercial production of tertiary relict treeLiquidambar orientalis. Abstract: The Liquidambar orientalis Miller is an important tree as a tertiary relict endemic species in terms of plant biodiversity, and has economic value due to the balsam it produces. In the present study, an efficient micropropagation method was developed for the L. orientalis. Initially, primordial shoot explants isolated from axillary buds were cultured on Murashige and Skoog and Woody Plant Medium (WPM) containing different plant growth regulators (PGRs). The highest number of shoots per explant was obtained on WPM supplemented with 1.0 mg/L 6-benzylaminopurine (BAP) and 1.0 mg/L indole-3-butyric acid (IBA) (shoot proliferation medium: SPM). These shoots were then subcultured continuously on SPM for a period of 18 months. Meristematic nodule clusters were formed at the base of the cultured shoots in SPM, where multiple shoots developed from them. The number of shoots per explant was increased approximately 2.8-fold by applying various strategies such as different explant type (single shoots and shoot clusters) and culture vessels (Glass Tube, 210-cc Glass culture jar, 400-cc Glass culture jar, and Vitro Vent®). Shoot clusters cultured in ventilated Vitro Vent® vessels on SPM gave best result for shoot propagation. In vitro shoots rooted best on WPM containing 30 g/L sucrose, 120 mg/L ethylenediamine di-2-hydroxyphenyl acetate ferric (Fe-EDDHA) and 4.0 mg/L IBA. All plantlets were successfully acclimatized in a glasshouse and then plants were transferred to the field. This methodology has been adapted by a commercial producer. © 2015, Springer-Verlag Berlin Heidelberg.Öğe Plant growth regulator effects on in vitro propagation and stevioside production in Stevia rebaudiana bertoni(American Society for Horticultural Science, 2016) Röck-Okuyucu B.; Bayraktar M.; Akgun I.H.; Gurel A.Stevia rebaudiana is of great importance due to its steviol glycosides (SGs) which are natural sweeteners used by the food industry as well as having medicinal purposes. In the present study, the effect of plant growth regulators (PGRs) and explant types on in vitro propagation and shoot growth of S. rebaudiana were studied, the effect of PGRs on SGs production was determined. For this purpose, nodal explants and shoot tip explants were cultured on woody plant medium (WPM) supplemented with cytokinins [6-benzyladenine (BA), kinetin (Kn), or thidiazuron (TDZ)] or cytokinins + auxins combinations [BA + indoleacetic acid (IAA); BA + naphthaleneacetic acid (NAA); Kn + IAA; Kn + NAA]. Although, the best shoot proliferationwas obtained onWPMsupplementedwith BA+ NAA combinations, shoots grown on PGR-containing media produced callus at the base of the shoots and showed chlorosis and necrosis. Additionally, shoots showed at all concentrations of TDZ, and at higher concentrations of BA, morphological changes such as malformed leaves and poor shoot growth. In contrast to PGR-containing media, on the PGR-free control medium, the development of shoots and roots occurred simultaneously and healthy and well-developed plantlets were obtained. Thus, we developed an economical viable means of in vitro propagation byminimizing themicropropagation steps and removing the requirement of PGRs. According to the high-performance liquid chromatography (HPLC) results, PGR-free control medium (WPM) led to considerably higher stevioside content in the leaves compared with the PGR(s)-containing media and the highest stevioside [34 mg·g-1 dry weight (DW)] and rebaudioside A content was only detected on the control medium without PGRs. Steviolbioside, rubusoside, and dulcoside A were detected qualitatively in the leaves of shoots grown on WPM supplemented with 2.27 µM TDZ, 4.54 µM TDZ, 2.22 µM BA + 2.69 µM NAA, 2.22 µM BA + 5.37 µM NAA, 2.32 µM Kn + 5.71 µM IAA, or 2.32 µM Kn + 2.69 µM NAA. © 2016, American Society for Horticultural Science. All rights reserved.Öğe Qtl analysis of fiber color and fiber quality in naturally green colored cotton (Gossypium hirsutum L.)(Society of Field Crops Science, 2015) Cuming D.S.; Altan F.; Akdemir H.; Tosun M.; Gurel A.; Tanyolac B.Improving fiber quality of naturally colored cotton has become more important in recent years due to changes in the spinning technology and consumer demand. The identification of quantitative trait loci (QTL) closely linked to the fiber quality traits can allow marker assisted selection (MAS) which leads us to future’s improved cotton cultivars. In this study, we performed genetic mapping on F2 population of G. hirsutum (Yesil × Nazilli 84) intraspecific cross, and identified QTL for fiber color parameters (L, a, b, ?L, ?a, ?b, ?E) and fiber quality traits (fiber length, uniformity and elongation). The resulting genetic linkage map comprised of 123 amplified fragment length polymorphism (AFLP) markers and 27 linkage groups (LGs), covering 2068.5cM with an average distance of 16.8cM between two markers. Using single marker analysis a total of 43 QTL for fiber color parameters and fiber quality traits were identified, including four for fiber length, two for fiber uniformity, two for fiber elongation, five for L, four for a, four for b, eight for ?L, four for ?a, four for ?b and six for ?E. The identified QTL for fiber color parameters and fiber quality traits explained between 7.8% and 14.6% and between 5.9% and 14.7% of the phenotypic variation, respectively. Additionally, recombinant individuals having green fiber color together with long fiber length, high fiber uniformity and elongation were obtained in the segregating population. These individuals can be used to develop new cotton varieties in the future. © 2015, Society of Field Crops Science. All rights reserved.Öğe Some agronomic and quality characteristics of two Achillea asplenifolia Vent. Populations(Society of Field Crops Science, 2016) Sonmez C.; Bayram E.; Tatar O.; Ekren S.; Gurel A.; Gevrek M.N.; Edreva A.; Vitkova A.The genus of Achillea L. consists of herbaceous plants are generally distributed in the northern hemisphere. Achillea asplenifolia and Achillea collina are the most important group of A. millefolium due to their highly effective pharmacological properties. Both species, A. asplenifolia and A. collina are distributed in the Europen flora but they don’t exist in the Turkish flora. The present study aimed to determine some yield and quality characteristics of two populations, Achillea asplenifolia - 9602 and Achillea asplenifolia – 10403, belonging to Achillea asplenifolia Vent. collected from the flora of Bulgaria at Bornova and to select genotypes with high yield and quality. For this purpose a two years of field experiment was conducted in 2009 and 2010. Plant height (cm), green herbage yield (g/plant), drug herbage yield (g/plant), drug flos yield (g/plant) and essential oil content of populations were determined. Based on the results of two years of experiment minimum and maximum drug flos yield were 2 g/plant and 391.4 g/plant in Achillea asplenifolia - 9602. Essential oil rate ranged between 0.050-3.30%. Minimum and maximum drug flos yield of Achillea asplenifolia (10403) were 0 and 159.8 g/plant respectively and essential oil content ranged between 0-2.5%. A large distribution was found in both population of Achillea asplenifolia and especially population Achillea asplenifolia - 9602 was more remarkable and promising for selection studies. © 2016, Society of Field Crops Science. All rights reserved.
