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Öğe Genetic confirmation of the occurrence and notes on the ecology of the yellow-bellied toad, Bombina variegata (L., 1758) (Amphibia: Bombinatoridae) in the European part of Turkey(British Herpetological Society, 2017) Karis M.; Veith M.; Gocmen B.; Oguz M.A.; Sener D.; Kurt B.We confirmed the occurrence and report some ecological traits of the yellow-bellied toad, Bombina variegata from Turkish Thrace. The specimens were caught in April 2016 from ponds and several streams of Karacahasan Mountain, Enez District, Edirne Province. In order to determine and confirm its systematic position, we genetically analysed the tissue samples of caught specimens. We also present some behavioural data of the species.Öğe In vitro cytotoxic and proapoptotic activities of anatolian Macrovipera lebetina obtusa (Dwigubski, 1832) crude venom on cultured K562 human chronic myelogenous leukemia cells [Anadolu’da yayiliş gösteren Macrovipera lebetina obtusa (Dwigubski, 1832) zehrinin k562 insan kronik myeloid lösemi hücreleri üzerinde in vitro sitotoksik ve apoptotik aktiviteleri](UHOD - Uluslararasi Hematoloji Onkoloji Dergisi, 2016) Suzergoz F.; Igci N.; Cavus C.; Yildiz M.Z.; Coskun M.B.; Gocmen B.In the context of searching for anticancer compounds in natural products, snake venom is one of the important sources for peptide/ protein based bioactive molecules. Proteins and peptides with anticancer activity were purified and identified from snake venoms. The aim of the present study was to determine the in vitro cytotoxicity of Macrovipera lebetina obtusa (Blunt-Nosed Viper) crude venom from southeastern Anatolia against K562 human chronic myelogenous leukemia (CML) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Adenosine tripohsphate (ATP) assays. Additionally, the apoptosis induction was assessed by morphological evaluation and immunohistochemical analysis for activated caspase-3. For histopahtological evaluation, haematoxylineosin, giemsa and papanicolau stains were used in combination. M. l. obtusa venom showed dose-dependent toxicity against K562 cells after 72 h treatment with different concentrations of crude venom. IC50 values were 0.45 and 0.37 µg/mL for MTT and ATP assays, respectively. Nuclear fragmentation and condensation, apoptotic bodies and activation of caspase-3, as an induction of apoptosis were also observed in K562 cells. Since apoptosis-inducing compounds are important for the treatment of cancer, further studies on Anatolian M. l. obtusa venom could result in the purification and identification of new proteins and peptides, which might have therapeutic value for the treatment of CML. © 2016, UHOD - Uluslararasi Hematoloji Onkoloji Dergisi. All rights reserved.Öğe New rumen ciliates from Turkish domestic cattle (Bos taurus L.) I. The presence of entodinium dalli Dehority, 1974 with a new forma, E. dalli f. rudidorsospinatum n. f. and comparisons with Entodinium williamsi n. sp.(Elsevier GmbH, 1996) Gocmen B.; Oktem N.In the course of examining rumen contents obtained from 25 domestic cattle (Bos taurus) in the vicinity of Izmir, Turkey, two somewhat similar but unusual forms of entodinia were observed. These ciliates were observed in three of the animals and this study reports on their classification into two species, Entodinium dalli Dehority, 1974 and a new species, Entodinium williamsi n. sp. All the protozoa identified as belonging to E. dalli were distinguished as a new forma, E. dalli f. rudidorsospinatum n. f. of the basis of their caudal spines. The species Entodinium williamsi n. sp. was also divided into two formae, E. williamsi f. williamsi n. f. and E. williamsi f. turcicum n. f. The morphological characteristics of these protozoa are described and their relationships to similar entodiniid species are discussed.
