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Öğe Anti-Trichomonas vaginalis activities of some Lamiaceae plants(Marmara Univ, 2022) Sumer Tuzun, Burcu; Fafal, Tugce; Tileklioglu, Evren; Ertabaklar, Hatice; Kivcak, BijenTrichomoniasis is a sexually transmitted infection caused by the Trichomonas vaginalis, non-viral protozoan, affecting the world. Metronidazole is one of the effective treatments approved by the FDA. Natural alternative approaches are required due to developed of resistance and drug intolerance in long-term treatment. The Lamiaceae, with 250 genera and 3000 species, are one of the largest family based on the taxon numbers. Thymus, Salvia, and Phlomis are also the largest genus of the family. The most distinctive feature of the species is active compounds of antiprotozoal activities. In present study Phlomis armeniaca Willd., Phlomis pungens Willd. var. pungens, Phlomis sieheana Rech. Fil., Thymus haussknechtii Velen., Thymus kotschyanus Boiss. & Hohen. var. kotschyanus, Stachys lavandulifolia Vahl subsp. lavandulifolia and Salvia verticillata L. subsp. verticillata L. had investigated anti-Trichomonas vaginalis activity. Results indicated that Thymus haussknetchtii was found anti-trichomonal activity in 250 and 125 mu g/ml concentrations at the end of 16 hours. The inhibition of Salvia verticillata subsp. verticillata and Phlomis sieheana. were started after 24 hours, and anti-trichomonal activity was detected 70% and 90% at the end of 48 hours, respectively.Öğe Antioxidant, tyrosinase inhibitor, and cytotoxic effects of Anthemis aciphylla Boiss. var. aciphylla and Cota dipsacea (Bornm.) Oberpr. & Greuter(Istanbul Univ, Fac Pharmacy, 2023) Tuzun, Burcu Sumer; Fafal, Tugce; Ilhan, Recep; Kivcak, Bijen; Kirmizibayrak, Petek BallarBackground and Aims: The study aims to examine the antioxidant , tyrosinase inhibitory, and cytotoxic activities of the methanol and chloroform extracts of Anthemis aciphylla Boiss. var. aciphylla and Cota dipsaceae (Bornm.) Oberpr. & Greuter in order to evaluate the results regarding different polarities.Methods: The study evaluates the total phenolic and total flavonoid content of the extract sand the in vitro antioxidant activi-ties using the DPPH, ABTS, superoxide radical, and nitric oxide scavenging activities. Tyrosinase inhibitor activity was identi-fied by Masuda's method. Cytotoxicity was investigated using the MTT assay.Results: The A. aciphylla var. aciphylla methanol extract (AAM) was the most active extract in term of overall antioxidant activity. The IC50 values for DPPH, ABTS, and superoxide anion radical activity were found to be 19.57, 46.82, and 5.610 mu g/ mL, respectively. Nitric oxide scavenging activity percent inhibition of the AAM was 46.47%. The tyrosinase inhibitory activity of AAM, was also the most effective, thus confirming the study's hypothesis. Moreover, no cytotoxic activity was observed to be present in the extracts with biological activity.Conclusion: According to the hypothesis, the tyrosinase inhibitor effect should be observed mostly in extracts and com-pounds that show the high antioxidant activity mostly originating from phenolic substances. AAM confirmed this hypothesis as the most active extract regarding both the tyrosinase inhibitor effect as well as antioxidant activities. Furthermore, the MTT assay was used to find AAM to be non-cytotoxic at the tested concentrationsThis indicates AAM to be able to be used more reliably in medicine and as a food preservative.Öğe Assessment of antioxidant and neuroprotective activity of plants from the Lamiaceae family(Istanbul Universitesi, 2024) Fafal, Tugce; Kayalar, Husniye; Birim, Dervis; Armagan, Guliz; Tuzun, Burcu Sumer; Tastan, Pelin; Kivcak, BijenBackground and Aims: Plants from the Lamiaceae family are shown to have pharmacological activities, including antiinflammatory, antidiabetic, antiasthmatic, hypolipidemic, antibacterial. This research measured the antioxidant and neuroprotective capacity of methanol extracts from the Mattubium vulgate L., Phlomis atmeniaca Willd., Thymus haussknechtii Velen. and, Thymus kotschyanus Boiss. & Hohen plants in cellular and cell-free systems. Methods: The neuroprotective potential of Mattubium vulgate, Phlomis atmeniaca, Thymus haussknechtii, and T. kotschyanus were determined against H2O2 toxicity in SH-SY5Y, the human neuroblastoma cell line. The antioxidant capacity of methanol extracts was examined by radical scavenging assays using static exempt chemical groups, 2.2'-diphenyl-1-picrylhydrazyl-hydrate (DPPH) and 2.2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Folin-Ciocalteau and Aluminum chloride assays were used to measure phenolic and flavonoid contents of the extracts. Results: Following these experiments, the effect of three different concentrations of extracts (1, 10 and 100 mu g/mL) on cell viability was assessed by a WST-1 [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium)]. The methanol extract of Mattubium vulgate was found to have the highest antiradical activity against DPPH (76.439 +/- 0.42%). The Thymus kotschyanus (10 mu g/mL) protected cells from H2O2-induced toxicity in all the extracts (p<0.05). Conclusion: In our report, we suggest that Thymus kotschyanus have potential neuroprotective activity because of the existence of polyphenolic compounds, flavonoids, and phenolic acids.Öğe Comparing the Fatty Acid Composition and Neuroprotective Effects of Some Lamiaceae Taxa from Turkey(Springer, 2022) Fafal, Tugce; Kaftan, Gizem; Tastan, Pelin; Kivcak, Bijen; Armagan, GulizThis study aims to analyze the fatty acid composition and neuroprotective potential of some selected Lamiaceae taxa from Turkey. For this purpose, methanol extracts of Lamium album L., Lamium garganicum L., Phlomis rigida Labill., Salvia syriaca L., Salvia sclarea L., and Stachys cretica subsp. anatolica Rech. f. were investigated for their activity against hydrogen peroxide induced neuronal cell death. The fatty acid compositions were analyzed by GC-FID, and the IA and IT indices and the omega 3/omega 6 ratio were also calculated. Saturated and unsaturated fatty acids of selected Lamiaceae species were measured to range from 8.92-17.59% and 75.13-84.57%, respectively. Phlomis rigida with the highest omega 3/omega 6 was shown to have the highest neuroprotection against H2O2-induced cell toxicity.Öğe Cytotoxic effects of silver nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract(Marmara Univ, 2023) Tastan, Pelin; Fafal, Tugce; Tuezuen, Burcu Suemer; Ozmen Yelken, Besra; Kayabasi, Cagla; Yilmaz Susluer, Sunde; Gunduz, CumhurThe aim of the study is to examine the cytotoxic, apoptotic effects and gene expressions of Asphodelus aestivus water extract (ASP) and silver nanoparticles (AgNPS) on decided cancer lines. Breast cancer cell lines MCF-7 and MDA-231; melanoma cancer lines MEWO and CHL-1; fibroblast cancer lines WI-38 and HEL 299 were selected for biological activities. xCELLigence system was used for cytotoxicity. Annexin V-EG FP Apoptosis detection kit was used for apoptosis and gene expressions were assessed by real time online RT-PCR by using cancer cell lines Qiagen kits. AgNPS showed significant cytotoxicity in all cell lines. The most prominent apoptosis was determined in MCF-7 cell line for AgNPS. It has been observed that the most important progress in gene expression, the suppression capacity of MUC1-C, a breast cancer-associated oncoprotein, is greatly increased by nanoparticle formation. In addition, cells that were not affected at all in MDA-MB-231 breast cancer oncoprotein started to suppress with nanoparticles. In conclusion, it was determined that silver nanoparticles increased the effect especially in breast cancer cell lines and could be considered for use.Öğe Eco-friendly Sensors Developed by Herbal Based Silver Nanoparticles for Electrochemical Detection of Mercury (II) Ion(Wiley-V C H Verlag Gmbh, 2019) Eksin, Ece; Erdem, Arzum; Fafal, Tugce; Kivcak, BijenIn our study, the single-use & eco-friendly electrochemical sensor platform based on herbal silver nanoparticles (AgNPs) was developed for detection of mercury (II) ion (Hg2+). For this purpose, the surface of pencil graphite electrode (PGE) was modified with AgNPs and folic acid (FA), respectively. The concentrations of AgNPs and FA were firstly optimized by differential pulse voltammetry (DPV) to obtain an effective surface modification of PGE. Each step at the surface modification process was characterized by using cyclic voltammetry (CV) and electrochemical impedence spectroscopy (EIS). The limit of detection (LOD) for Hg2+ was estimated and found to be 8.43 mu M by CV technique. The sensor presented an excellent selectivity for Hg2+ against to other heavy metal ions such as Ca2+, Cd2+, Cr3+, Cu2+, Mg2+, Ni2+, Pb2+, Zn2+, Co2+ and Mn2+. Moreover, a rapid, selective and sensitive detection of Hg2+ was successfully performed in the samples of tap water within 1 min.Öğe Elemental Analysis And Antioxidant Activities Of Tripleurospermum parviflorum (Willd.) Pobed. And T. tenuifolium (Kit.) Freyn Ex Freyn(Mattioli 1885, 2021) Fafal, TugceIn this study, we determined the trace element contents of Tripleurospermum parviflorum (Willd.) Pobed. and T. tenuifolium (Kit.) Freyn ex Freyn from Izmir, Turkey. The complete phenolic and flavonoid contents, as well as the antioxidant properties of methanol extracts of Tripleurospermum parviflorum and T. tenuifolium aerial parts, were also examined. Nine minerals (Na, Ca, Mg, K, Si, Al, P, S and Ti), six trace elements (Fe, Zn, Ni, Br, Ag and Ta) were determined. The heavy metal content in percentages were determined as 9.78-11.08% and 21.63-19.94% for powdered drug and water extracts of Tripleurospermum parviflorum and T. tenuifolium, respectively. T. tenuifolium water extract was found to posses highest concentration of K. In addition, Na, Cl and Ca were also obtained in significant amounts in water extracts of two species. The presence of K and Ca in examined two Tripleurospermum species might contribute to explain the traditional use of these drugs against cardiac disorders. The methanolic extract of T. parviflorum demonstrated the higher antioxidant activity than T. tenuifolium. The antioxidant activity assay findings were found to have significant correlation with the total phenolics and flavonoid content of the extracts.Öğe Evaluation of the phenolic compounds and the antioxidant potentials of Vitex agnus-castus L. leaves and fruits(Walter De Gruyter Gmbh, 2022) Kimna, Ceren; Fafal, TugceObjectives In this study, we aim at deciphering the phenolic content of Vitex agnus-castus L. leaf and fruit extracts prepared with different methods and relate it to their antioxidant activity. Methods In this study, phenolic compounds and the antioxidant potential of the ethanol fruit and leaf extracts of V. agnus-castus L. (Chaste tree) were evaluated spectrometrically. Furthermore, selected polyphenols, i.e., chlorogenic acid and rutin, were determined by the HPLC-DAD method qualitatively and quantitatively. Results The results obtained from leaf and fruit extracts were compared with a commercial product (CP) containing the fruit extract of V. agnus-castus. Leaf extract was found to be richer in flavonoids when compared to the fruit counterparts. Accordingly, they also showed higher antioxidant activity. Conclusions Extracts prepared here can be considered as promising antioxidant agents for future therapeutic formulations.Öğe FATTY ACID COMPOSITION AND ANTIOXIDANT ACTIVITY OF Ranunculus constantinopolitanus(Springer, 2022) Say, Deniz; Tastan, Pelin; Fafal, Tugce[No Abstract Available]Öğe Fatty acids and antioxidant capacities of three Centaurea L. species(Marmara Univ, 2022) Tastan, Pelin; Tuzun, Burcu; Kivcak, Bijen; Fafal, TugceThe aim of this study is to determine the fatty acid compositions and antioxidant activities of three Centaurea species as Centaurea iberica, C. urvillei ssp. hayekiana, and C. urvillei ssp. nimrodis and compare them with each other. As a result of fatty acid determination by GC MS method, oleic acid and linoleic acid were found as the main fatty acids in these Centaurea species. Polyunsaturated fatty acids (PUFAs) were determined at 58.66%, 46.98%, 62.22% in C. iberica, C. urvillei ssp. hayekiana and C. urvillei ssp. nimrodis, respectively. Antioxidant activities of methanolic extracts of three Centaurea species were evaluated by DPPH, ABTS and CUPRAC assays. The methanol extract of C. urvillei ssp hayekiana showed the highest antioxidant activity in direct proportion to the total amount of phenolic and flavonoid substances. The fact that the antioxidant activities of these three species and fatty acid analyzes have not been compared in previous studies is important in that this study is the first.Öğe Green Synthesis of Silver Nanoparticles by Using Anthemis Tricolor Boiss., Factorial Design for Parameter Optimization, Characterization and In-Vitro Biological Activities(Springer, 2024) Tuzun, Burcu Sumer; Fafal, Tugce; Ozguney, Isik; Kivcak, BijenPurpose The objectives of the study are to obtain silver nanoparticles using Anthemis tricolor in an environmentally friendly, economical, and more effective way with green synthesis and also to determine the most appropriate parameters by examining the effects of preparation conditions on particle size, polydispersity index, and zeta potential using factorial design and further to compare the bioactivity results between water extract and silver nanoparticles. Thus, it is aimed to shed light on further studies on the lightening effects of nanoparticles on the skin. Methods In this study, silver nanoparticles were synthesized by reduction using Anthemis tricolor water extract and silver nitrate solution. Then, various characterization methods (Ultraviolet-Visible Spectroscopy, X-Ray Diffraction analysis) were studied. In addition, the effects of incubation temperature, silver nitrate solution concentration, and extract volume on particle size, zeta potential, and polydispersity index were investigated, and optimum parameters were determined. In addition, antioxidant activities of water extract and silver nanoparticles (DPPH Radical Scavenging Activity, ABTS Radical Cation Scavenging Activity, and Cupric Reducing Antioxidant Capacity) and tyrosinase inhibitory activities were determined. Results According to the results, it was observed that biosynthesis took place with the absorption peak at 440 nm. In addition, changes in peak intensities and shifts in values that occur at the end of nanoparticle formation were determined by Fourier Transform Infrared Spectroscopy. Cubic morphology was determined by Scanning Electron Microscopy. The existence of (111), (200), (220) and (311) planes was proved by X-ray diffraction analysis, confirming the existence of silver nanoparticles in a cubic structure. The optimized parameters were determined for silver nanoparticles obtained by incubating 5 ml of an extract with 1 mM silver nitrate solution at 25 degrees C. Polydispersity index, zeta potential, and particle size values were in the appropriate range. On the other hand, it was observed than silver nanoparticles' antioxidant and tyrosinase inhibitory activities were higher than those the water extract. Conclusion It was concluded that Anthemis tricolor could be presented as an effective, stable, reducing agent. It also suggested that it can be used due to its antioxidant and tyrosinase inhibitory activities and directed to further studies for skin-lightening preparations.Öğe Phlomis species protects the cells from oxidative stress by upregulating catalase enzyme expression(Innovhub Ssi-Area Ssog, 2022) Tastan, Pelin; Birim, Dervis; Fafal, Tugce; Armagan, Guliz; Kivcak, BijenRecently, interest in the pharmacological effects of plants has increased and studies on bioactive compounds have intensified. In this study, we aimed at evaluating the antioxidant and neuroprotective potential of Lamiaceae plants including Stachys lavandulifolia, Salvia verticillata, Phlomis herba venti sp. pungens and Phlomis sieheana in both cellfree systems and the cell culture model. Methanol extracts of the aerial parts of these plants, cultivated in Elazig, Turkey were carried out. Antioxidant activity assays (ABTS and DPPH) and total phenolic and flavonoid contents of the extracts were measured. Next, the effects of methanol extracts on cell viability and catalase protein levels were investigated in hydrogen peroxide induced oxidative stress model. DPPH radical scavenging activities were determined with the IC50 value of 31.36 +/- 0.25, 30.44 +/- 0.19, 30.16 +/- 0.08 and 56.54 +/- 0.93 for Stachys lavandulifolia, Salvia verticillata, Phlomis herba venti sp. pungens and Phlomis sieheana, respectively. ABTS radical scavenging activities were determined as 6.38 +/- 1.48, 16.93 +/- 0.68, 5.97 +/- 4.04 and 2.28 +/- 0.51. The highest cellular protection and catalase expression were determined in Phlomis-treated cells against H2O2 (p<0.05). On the other hand, no statistically significant change in cell viability was observed when the extracts were compared with each other. Our findings support the neuroprotective and antioxidant potential of these four Lamiaceae plants, particularly Phlomis species.Öğe Structural characterization, antioxidant and cytotoxic effects of iron nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract(Walter De Gruyter Gmbh, 2020) Tuzun, Burcu Sumer; Fafal, Tugce; Tastan, Pelin; Kivcak, Bijen; Yelken, Besra Ozmen; Kayabasi, Cagla; Gunduz, CumhurASP was used to synthesize FeNPA. They were characterized by UV-vis spectroscopy, FT-IR, TEM, SEM, XRD and ZP. the aim of this study was to evaluate in vitro cytotoxic activity and antioxidant acitivities of FeNPA and ASP. the antioxidant properties were evaluated using DPPH, ABTS(+) and H2O2 assays. FeNPA had higher antioxidant activity comparing to ASP according to DPPH (IC50: 3.48 mu g/mL) and ABTS(+) (60.52%) assays. Anti-cancer activities of FeNPA and ASP were investigated in breast cancer, melanoma and control cell lines. FeNPA was more cytotoxic than ASP in MCF-7, MeWo, CHL-1, and HEL 299 cells. FeNPA had shown that mitochondria induce apoptosis through stress in MDA-MB-231, and cells MeWo. ASP also induced apoptosis 2.23-fold in MCF-7 cells. Progesterone receptor gene expression showed a 10-fold increase in a hormone-dependent MCF-7 cell line in ASP, and FeNPA treatment. Expressions of BCL6, CXCL12, DNAJC15, RB1 and TPM1 in melanoma cancer cell lines were significantly increased in ASP and FeNPA administration. It had been shown that FeNPA regulates gene expressions that may be considered important in terms of prognosis in breast cancer and melanoma cell lines and it is suggested that gene expressions regulated by FeNPA are also evaluated in animal models in vivo.
