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Öğe Extraction, radiolabeling, and biodistribution of thebaine in rats(Springer Birkhauser, 2013) Enginar, H.; Unak, P.; Lambrecht, F. Yurt; Muftuler, F. Zumrut Biber; Seyitoglu, B.; Yurt, A.; Yolcular, S.; Medine, E. I.; Bulduk, I.The goal of this study was to determine the radiopharmaceutical potential of radioiodinated thebaine. Thebaine was extracted from dry capsules of opium poppy (Papaver somniferum L.), purified using high-performance liquid chromatography, and characterized with nuclear magnetic resonance and infrared spectroscopy. The purified thebaine was labeled with I-131 using the iodogen method. Normal and receptor-blockage biodistribution studies were performed in male Albino Wistar rats. The results of the tissue distribution studies showed that the uptake of I-131-thebaine in the stomach, large intestine, spinal cord, and prostate was higher than in the other tissues. A greater uptake of radiolabeled thebaine in the rat brain was observed in the midbrain and hypothalamus. We concluded that (1) the labeling yield of I-131-thebaine was high, (2) a large amount of I-131-thebaine remained in the midbrain and hypothalamus, and (3) I-131-thebaine had enough stability for diagnostic scanning.Öğe Radiolabeling of codeine with I-131 and its biodistribution in rats(Springer, 2009) Enginar, H.; Unak, P.; Lambrecht, F. Y.; Muftuler, F. Z. Biber; Medine, E. I.; Yolcular, S.; Yurt, A.; Seyitoglu, B.; Bulduk, I.Codeine which was extracted from dry capsules of the opium poppy (Papaver somniferum) was purified by HPLC (High Performance Liquid Chromatography) and characterized by NMR (Nuclear Magnetic Resonance) and IR (Infrared) spectroscopy techniques. The purified compound was labeled with I-131 and biodistribution studies were performed in rats. Radioiodinated codeine distributed uniformly in the cerebellum, m.pons, striatum and hypothalamus while the other branch of brain and Stomach, urinary bladder, and small intestine uptakes were significantly higher than other tissues.Öğe Synthesis, radiolabeling and biodistribution of morphine glucuronide (mor-glu)(Springer, 2012) Enginar, H.; Unak, P.; Muftuler, F. Z. Biber; Lambrecht, F. Y.; Medine, E. I.; Yolcular, S.; Kilcar, A. Yurt; Seyitoglu, B.; Bulduk, I.The aim of this study was to synthesize a glucuronide conjugated morphine derivative which could be labeled with I-131, as a radiopharmaceutical, and to investigate its radiopharmaceutical potential using biodistribution studies in male Albino Wistar rats. Morphine was extracted from dry capsules of the opium poppy (Papaver somniferum L.). It was conjugated with UDP-glucuronic acid by using UDP-glucuronyl transferase (UDPGT) enzyme rich microsomes, purified by high performance liquid chromatography (HPLC) and characterized by nuclear magnetic resonance (NMR), infrared (IR) spectroscopy and liquid chromatography mass spectroscopy (LC-MS/MS). Normal and receptor blockage biodistribution studies were performed in male Albino Wistar rats. The results of the tissue distribution studies showed that I-131 labeled morphine glucuronide (I-131-mor-glu) uptake in the small intestine, large intestine and urinary bladder was higher than in the other tissues of the rats in the blocked receptor and unblocked receptor. A greater uptake of the radio labeled substance was observed in the hypothalamus and mid brain than in the other branches of the rats' brains.Öğe Synthesis, Radiolabeling and In Vivo Tissue Distribution of an Anti-Oestrogen Glucuronide Compound, Tc-99m-TOR-G(Int Inst Anticancer Research, 2010) Muftuler, F. Z. Biber; Unak, P.; Yolcular, S.; Kilcar, A. Yurt; Ichedef, C.; Enginar, H.; Sakarya, S.Toremifene (TOR) has been used as an anti-oestrogen drug for the treatment and prevention of human breast cancer. The aim of this study was the addition of the hydrophilic groups diethylenetriamine pentaacetic acid (DTPA) and glucuronic acid to the starting substance TOR and to label it with technetium-99m (Tc-99m) radionuclide and to investigate radiopharmaceutical potential of the new compound. The synthesis reactions are completed in four steps, including enzymatic reaction, with the following sub-steps; preparation of microsomal fraction from Hutu 80 cell line and subsequent purification of UDP-glucuronyl transferase (UDPGT), estimation of protein quantity in microsomal samples and glucuronidation reaction. The results indicate that Tc-99m-TOR-G may be proposed as a new anti-oestrogen glucuronide imaging agent for ovarian tumours.