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Öğe Biodistribution of (99m)technetium-labeled creatinine in healthy rats(Assoc Bras Divulg Cientifica, 2007) Yilmaz, O.; Lambrecht, F. Yurt; Soylu, A.; Durkan, K.; Kavukcu, S.The distribution of creatinine, one of the toxic guanidine compounds, in various tissues has not been studied in detail by using radiolabeled creatinine. Our objective was to investigate the biodistribution of creatinine labeled with (99m)technetium (Tc-99m) by the stannous (II) chloride method in healthy male Wistar rats. Quality controls were carried out by radio thin layer chromatography, high- performance liquid chromatography, and paper electrophoresis. The labeling yield was 85 +/- 2% under optimum conditions (pH 7 and 100 mu g stannous chloride). Rats (N = 12) were injected intravenously with Tc-99m-creatinine and their blood and visceral organs were evaluated for Tc-99m-creatinine uptake as percent of the injected dose per gram wet weight of each tissue (% ID/g). The lowest amount of uptake was detected in the brain and testis. When the rate of uptake was evaluated, only the kidney showed increasing rates of uptake of Tc-99m-creatinine throughout the study. Kidneys showed the highest amount of uptake throughout the study (P < 0.001 compared to all other organs), followed by liver, spleen and lung tissue.Öğe Distribution of I-131-labeled recombinant human erythropoietin in maternal and fetal organs following intravenous administration in pregnant rats(Springer, 2007) Yilmaz, O.; Lambrecht, F. Y.; Gokmen, N.; Erbayraktar, S.; Durkan, K.The aim of the present study was to demonstrate the possible transplacental transmission of I-131 labeled recombinant human erythropoietin (I-131-rh-EPO) in pregnant rats and its distribution through maternal and fetal organs. Six Wistar Albino Rats in their pregnancy of 18 days were used I-131 labeled recombinant human erythropoietin (specific activity = 2.4 mu Ci/IU) was injected into the tail vein of rats. After 30 minutes labeled erythropoietin infusion maternal stomach, kidney, lung, liver, brain and heart as well as fetus were removed. Then, the same organs were removed from each fetus. Measuring weight of maternal and fetal organs as well as placenta were followed by radioactivity count via Cd(Te) detector. I-131 labeled recombinant human erythropoietin was found to be able to pass rat placenta and its distribution order in fetal organs was similar to those of maternal organs. Besides, as measurements were performed closer to cornu uteri, uptakes were decreasing in every fetus and its corresponding placenta.Öğe Evaluation of 99mTc-Cefuroxime axetil for imaging of inflammation(Springer, 2007) Lambrecht, F. Yurt; Yilmaz, O.; Uenak, P.; Seyitoglu, B.; Durkan, K.; Baskan, H.Öğe Labeling bombesin-like peptide with Tc-99m via hydrazinonicotinamide: description of optimized radiolabeling conditions(Springer, 2010) Lambrecht, F. Yurt; Durkan, K.; Bayrak, E.Bombesin (BNN)-like peptides have very high binding affinity for the gastrin-releasing peptide (GRP) receptor. The goal of the current study was to optimize the labeling conditions of a new Tc-99m-radiolabeled BNN-like peptide based on the bifunctional chelating ligand HYNIC using different co-ligands (EDDA and tricine). The radiolabeling conditions (pH, amount of co-ligand, amount of stannous chloride, temperature and reaction time) for newly-formed Tc-99m-tricine-HYNIC-Q-Litorin and Tc-99m-EDDA-HYNIC-Q-Litorin were optimized and evaluated by RHPLC and RTLC. Radiochemical yields for Tc-99m-tricine-HYNIC-Q-Litorin and Tc-99m-EDDA-HYNIC-Q-Litorin were 98.0 +/- A 1.7 and 97.5 +/- A 2.5%, respectively. When EDDA was used as co-ligand, the labeling of Tc-99m-EDDA-HYNIC-Q-Litorin was optimal in the following reaction mixture: HYNIC-peptide: EDDA: 10 mu g/5 mg, pH 3, SnCl2 concentration: 12 mu g/0.1 mL, reaction temperature: 100 A degrees C, reaction time: 15 min. Besides, the optimum conditions were HYNIC-peptide:tricine: 10 mu g/50 mg, pH 5, SnCl2 concentration: 12 mu g/0.1 mL, reaction temperature: 100 A degrees C, reaction time: 15 min for preparing Tc-99m-tricine-HYNIC-Q-Litorin. The manufactured Tc-99m-HYNIC-Q-Litorin conjugates may offer new possibilities for imaging cancer cells expressing bombesin receptors.Öğe Labeling of apigenin with I-131 and bioactivity of I-131-apigenin in male and female rats(Springer, 2009) Seyitoglu, B.; Lambrecht, F. Yurt; Durkan, K.Apigenin (4',5,7-trihydroxyflavone), one of the most common flavonoids, has been shown to possess a variety of biological activities including tumor growth inhibition and chemopreventation. In the present study, apigenin was labeled with I-131 using iodogen method and investigated of its bioactivity. Radiolabeling yield is 98 +/- 0.2%, as determined by radio thin layer chromatography (RTLC), electrophoresis and radio high performance liquid chromatography (RHPLC). Besides, structure analysis of synthesized cold iodoapigenin complex were assessed with LCMS/MS and H-1-NMR. Results of in vitro study indicated a high stability (3 hours) in human serum. Biodistrubition studies are performed in male and female albino Wistar rats. Biodistribution data related to the male rats showed significant uptake in the small intestine. The female rats biodistribution results indicated that the uptake of I-131-apigenin was high in the intestine and uterus.Öğe Preparation and biodistribution of [I-131]linezolid in animal model infection and inflammation(Springer, 2009) Lambrecht, F. Yurt; Yilmaz, O.; Durkan, K.; Unak, P.; Bayrak, E.Linezolid is the first of new class of antibiotics, the oxazolidinones, and exhibits activity against many gram-positive organisms, including vancomycin-resistant Enterococcus faecium, methicillin-resistant Staphylococcus aureus, and penicillin-resistant Streptococcus pneumoniae. Aim of the study: Linezolid was to label with I-131 and potential of the radiolabeled antibiotic was to investigate in inflamed rats with S. aureus ( S. aureus) and sterile inflamed rats with turpentine oil. Linezolid was labeled with I-131 by iodogen method. Biodistribution of [I-131] linezolid was carried out in bacterial inflamed and sterile inflamed rats. Radiolabeling yield of [I-131] linezolid was determined as 85 +/- 1% at pH 2. After injecting of [I-131] linezolid into bacterial inflamed and sterile inflamed rats, radiolabeled linezolid was rapidly removed from the circulation via the kidneys. Binding of [I-131] linezolid to bacterial inflamed muscle (T/NT = 77.48 at 30 min) was five times higher than binding to sterile inflamed muscle (T/NT = 14.87 at 30 min) of rats. [I-131] linezolid showed good localization in bacterial inflamed tissue. It was demonstrated that [I-131] linezolid can be used to detect S. aureus inflammation in rats.Öğe Preparation and biodistribution of [I-131]linezolid in animal model infection and inflammation(Springer, 2009) Lambrecht, F. Yurt; Yilmaz, O.; Durkan, K.; Unak, P.; Bayrak, E.Linezolid is the first of new class of antibiotics, the oxazolidinones, and exhibits activity against many gram-positive organisms, including vancomycin-resistant Enterococcus faecium, methicillin-resistant Staphylococcus aureus, and penicillin-resistant Streptococcus pneumoniae. Aim of the study: Linezolid was to label with I-131 and potential of the radiolabeled antibiotic was to investigate in inflamed rats with S. aureus ( S. aureus) and sterile inflamed rats with turpentine oil. Linezolid was labeled with I-131 by iodogen method. Biodistribution of [I-131] linezolid was carried out in bacterial inflamed and sterile inflamed rats. Radiolabeling yield of [I-131] linezolid was determined as 85 +/- 1% at pH 2. After injecting of [I-131] linezolid into bacterial inflamed and sterile inflamed rats, radiolabeled linezolid was rapidly removed from the circulation via the kidneys. Binding of [I-131] linezolid to bacterial inflamed muscle (T/NT = 77.48 at 30 min) was five times higher than binding to sterile inflamed muscle (T/NT = 14.87 at 30 min) of rats. [I-131] linezolid showed good localization in bacterial inflamed tissue. It was demonstrated that [I-131] linezolid can be used to detect S. aureus inflammation in rats.Öğe Preparation of 131I-Pyrimethamine and Evaluation for scintigraphy of experimentally Toxoplasma gondii infected rats(Springer, 2012) Lambrecht, F. Yurt; Inceboz, Tonay; Surucu, E.; Yilmaz, O.; Yavasoglu, A.; Durkan, K.; Baykara, B.; Bekis, R.; Uner, A.Öğe Preparation, quality control and stability of Tc-99m-cefuroxime axetil(Springer, 2008) Lambrecht, F. Yurt; Durkan, K.; Unak, P.Cefuroxime axetil, a cephalosporin antibiotic used to treat bacterial infections, was investigated to label with Tc-99m. Radiolabeling of cefuroxime axetil was carried out by using stannous chloride method. Effects of pH and stannous chloride amount on the radiolabeling yield were investigated. The radiochemical purity of Tc-99m-cefuroxime axetil was determined by thin layer radio chromatography (TLRC), electrophoresis and high performance liquid chromatography. The maximum radiolabeling yield was 98 +/- 1%.Öğe Technetium-99m labeled Mebendazole and Biodistribution in experimentally Trichinella spiralis-infected rats(Springer, 2009) Inceboz, T.; Lambrecht, F. Yurt; Yilmaz, O.; Kaya, G. Capa; Kolatan, E.; Durkan, K.; Bagriyanik, A.; Uner, A.Öğe Technetium-99m labeled Mebendazole and Biodistribution in experimentally Trichinella spiralis-infected rats(Springer, 2009) Inceboz, T.; Lambrecht, F. Yurt; Yilmaz, O.; Kaya, G. Capa; Kolatan, E.; Durkan, K.; Bagriyanik, A.; Uner, A.
