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Öğe Anti-tumor effects of bemiparin in HepG2 and MIA PaCa-2 cells(Nature Publishing Group, 2018) Alur, I.; Dodurga, Y.; Secme, M.; Elmas, L.; Bagci, G.; Goksin, I.; Biray-Avci, C.[No Abstract Available]Öğe Determination of T-cell clonality and expression profiles of Toll-like receptors signaling pathway genes and related miRNAs in patients with mycosis fungoides(Elsevier B.V., 2024) Seçme, M.; Dodurga, Y.; Demirkan, N.Ç.; Kaçar, N.; Günel, N.S.; Açıkbaş, İ.Cutaneous T-cell lymphomas (CTCL) encompass a group of diseases characterized by the presence of malignant clonal CD4+ T lymphocytes in the skin. Mycosis fungoides (MF) is the most prevalent form of CTCL, accounting for approximately 60 % of cutaneous T-cell lymphomas and 50 % of all primary cutaneous lymphomas. Despite ongoing research, the precise pathogenesis of MF remains incompletely understood. Toll-like receptors (TLRs) have the ability to specifically recognize ligands, subsequently induce the expression of diverse genes and activate innate immunity within the cell. Furthermore, miRNAs play a crucial role in regulating various aspects of immune cell function. The aim of our study was to explore the potential roles of TLRs and the genes implicated in their signal transduction, along with the expression status of miRNAs in the mechanisms underlying MF. Additionally, we assessed the clonal status and compared it with clinicopathological data using a T-cell clonality assay. To determine the expression status of TLR pathway genes and miRNAs, we conducted RT-PCR analysis on 52 MF samples and 50 control paraffin block materials. Pathway analysis were conducted using the KEGG database. T-cell receptor (TCR) gamma clonality changes were evaluated. Results from the study revealed increased expressions of TLR-1, -4, -8, IRF7, TRAF3, MEK1, MEK2, Elk1, NFkB, hsa-miR-21-5p, and hsa-miR-155-5p, as well as decreased expressions of hsa-miR-130a-3p, hsa-miR-210-3p, and hsa-let-7e-5p in the MF group. TCR gamma clonal change analysis demonstrated that 55.5 % of the analysed DNAs exhibited monoclonal and biallelic patterns, while 45.5 % displayed polyclonality. These findings collectively suggest the potential influence and therapeutic possibilities of the TLR signalling pathway in the molecular pathogenesis of MF. © 2023 Elsevier B.V.Öğe DO EXPRESSIONS OF OLIG2, PTEN AND DMBT1 GENES PLAY A ROLE IN HIGH-GRADE GLIOMAS PROGRESSION?(Duke Univ Press, 2008) Avci, C. Biray; Oktar, N.; Dalbasti, T.; Yilmaz, S.; Dodurga, Y.; Dogan, Z. O.; Numanoglu, S.; Akalin, T.; Gunduz, C.Öğe Investigation of the effects of a sulfite molecule on human neuroblastoma cells via a novel oncogene URG4/URGCP(Wiley, 2017) Dodurga, Y.; Secme, M.; Eroglu, C.; Gundogdu, G.; Avci, C. Biray; Bagci, G.; Kucukatay, V.; Satiroglu-Tufan, N. L.; Avci, C. BirayÖğe Promoter hypermethylation-mediated down-regulation of RUNX3 gene in human brain tumors(Springer London Ltd, 2014) Avci, C. B.; Dodurga, Y.; Susluer, S. Y.; Sigva, Z. O. D.; Yucebas, M.; Caglar, H. O.; Akalin, T.; Dalbasti, T.; Oktar, N.; Gunduz, C.Background The Runx family proteins, including RUNX3, are tissue-restricted transcription factors and play role in neuronal development and tumorigenesis. RUNX3 has an important role in glioblastoma (GBM) tumorigenesis because of its promoter hypermethylation. Aim We aimed to evaluate the methylation-mediated expression regulation of RUNX3 gene in brain tumors. Patients and methods Cases of meningiomas WHO grade III (3), anaplastic astrocytomas (3), diffuse astrocytoma (3), and GBM (12) were recruited into this study. Real-time quantitative PCR was performed for analyses of DNA promoter methylation and analyses of methylation-mediated expression status of RUNX3 gene was performed by real-time quantitative RT-PCR. Results There was no significant difference between methylated and unmethylated quantitative ratio of RUNX3 gene promoter region and also no significant difference in relative ratio of RUNX3 gene expression in brain tumor groups. Methylated and unmethylated ratio in anaplastic astrocytoma, diffuse astrocytoma, GBM, meningioma (WHO grade III) and in all groups were; 1.44, 1.09, 1.51, 1.52 and 1.43, respectively. One allele was found methylated necessarily. No methylation was detected in one case of GBM group and one case of anaplastic astrocytoma group. There was no unmethylated promoter in one of the GBM cases. There were significant differences between relative ratio of RUNX3 gene expression and methylated/unmethylated ratio rates for all cases (p = 0.001) and GBM groups (p = 0.041). Conclusion This study overemphasized the RUNX3 gene importance in brain tumors, due to the existence of at least one methylated allele.Öğe PROMOTOR HYPERMETHYLATION-MEDIATED DOWN-REGULATION OF RUNX3 GENE IN HUMAN BRAIN TUMORS(Oxford Univ Press Inc, 2010) Avci, C. Biray; Dodurga, Y.; Oktar, N.; Yilmaz, S.; Sigva, Z. O. Dogan; Yucebas, M.; Cogulu, O.; Akalin, T.; Dalbasti, T.; Gunduz, C.
