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    Critical time point for apoptotic cell death in an experimental ischemia/reperfusion model and the effect of N-acetylcystein [Deneysel iskemi/reperfüzyon modelinde apoptotik hücre ölümü için kritik zamanlama ve N-asetilsistein’in etkisi]
    (Turkish Biochemistry Society, 2017) Ozsarlak-Sozer G.; Emre M.; Demirkol S.; Açıkalın A.; Çetiner S.; Topçu Z.; Demir-Dora D.
    Objective: Kidney transplantation is an important treatment option in end stage renal failure. Tissue death may be an important problem when a kidney is removed from a cadaver and transported to a donor a long distance away. The purpose of this study is to determine the critical time point for apoptotic cell death in a renal ischemia/reperfusion model and determine the effects of N-acetylcystein on apoptosis induced by ischemia injury. Methods: Apoptotic cell death after induced renal ischemia followed by reperfusion, was estimated in a group of Wistar albino rats by immunoflourescence and ELISA techniques. N-acetylcystein, an antioxidant agent, was given to the rats to study the effect on apoptosis. Tissues were examined immunohistochemically at 0, 1 h, 24 h, 5 days and 10 days for detection of apoptotic cells. Results: Our results showed that an ischemia for 60 min followed by reperfusion for 60 min triggered apoptosis. Moreover, N-acetylcystein significantly diminished both the ischemia/reperfusion damage and apoptosis. Conclusion: We anticipate our results would be important for kidney transplantation in estimating the critical time point for apoptosis and administration of N-acetylcystein prior to removal of the organ may be important in delaying the onset of apoptosis. © 2017, Turkish Biochemistry Society. All rights reserved.