Yazar "Coven, Furkan Ozan" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • Küçük Resim Yok
    Öğe
    Antiviral and cytotoxic activities and chemical profiles of two species of Abies nordmanniana from Türkiye
    (Tubitak Scientific & Technological Research Council Turkey, 2024) Sarikahya, Nazli; Okkali, Gaye Sumer; Coven, Furkan Ozan; Zeinal, Sevda; Gucur, Gulce; Caglar, A. Dila; Uyar, Ece
    Abies is an important genus of the family Pinaceae, with about 50 species found in the highlands of Asia, Europe, North Africa, and North and Central America. The principal aim of the present work was to investigate the chemical content and biological potential of the resin and cone from Abies nordmanniana subsp. bornmulleriana and Abies nordmanniana subsp. equi-trojani , respectively. The flavonoid and phenolic contents of the resin and cones were evaluated using liquid chromatography -high resolution mass spectrometry (LC-HRMS). Additionally, the essential oil and fatty acid compositions were analyzed using gas chromatography -mass spectrometry (GC -MS) and gas chromatography -flame ionization detector (GC-FID), respectively. Cytotoxicity of the extracts and essential oils were screened against certain cancer cell lines, namely, human prostate adenocarcinoma cell line (PC3), human lung adenocarcinoma cell line (A549), human pancreatic cancer cell line (PANC-1), human hepatocellular carcinoma cell line (HepG2), human breast cancer cell line (MDA-MB231), and normal human lung fibroblast cell line (CCD-34-LU), with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl2H-tetrazolium bromide] assay. According to the MTT results, hexane extracts of both cone (CH) and resin (RH), ethanol -water (CEW), dichloromethane (CD), and acetone (CA) extracts of the cone mostly inflict cytotoxicity in HepG2 cell line. Antiviral activities of Abies nordmanniana subsp. extracts at doses of 5 mu g/g and 10 mu g/g were also evaluated in ovo for their virucidal activity against avian coronavirus. Abies nordmanniana subsp. extracts exhibited concentration -dependent antiviral activity on specific pathogen -free embryonated chicken eggs. Significantly, cone acetone extract (CA), cone ethanol extract (CE), and cone dichloromethane extract (CD) of Abies nordmanniana subsp. exhibited strong inhibition of the virus at a concentration of 10 mu g/g. The most potent virucidal activity was observed with ethanol -water extract of conifer form (CEW). According to these results, it was proved that Abies nordmanniana species could be a potential, sustainable, and renewable drug source, especially considering the impressive antiviral and significant cytotoxic activity potentials.
  • Küçük Resim Yok
    Öğe
    Assessment of In-vitro Cytotoxicity and In-ovo Virucidal Antiviral Efficacy of Various Plant Extracts and Bioactive Molecules
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2024) Coven, Furkan Ozan; Gur, Seray; Uyar, Ece; Alsakini, Karrar Ali Mohammed Hasan; Karabey, Fatih; Coven, Fethiye; Caliys, Ihsan
    The viral diseases that occurred in recent years have increased the interest in non-toxic to healthy cells and naturally isolated agents to struggle with these diseases. The key intention of this research is to examine both antiviral potentials against the Infectious Bronchitis model virus (IBV) and cytotoxic activities on determined cell lines of different active ingredients and medical herbs extracts for developing new antiviral agents or drugs towards SARS-CoV-2. The antiviral potency of the samples against IBV was determined as in ovo virucidal antiviral activity in specific pathogen-free (SPF) embryonated chicken eggs (ECEs). To detect antiviral activity, the haemagglutination test was performed after 48 h of incubation for all samples. The cytotoxic activity of the samples was identified on HepG2, Caco-2, HeLa, HEK293, PANC-1, PC-3, A549, MDAMB-231, and CCD-34Lu cell lines by the MTT protocol. Hypericum perforatum extract was found to have a dominant role in cytotoxicity and antiviral activity. In addition, while nobiletin and Sambucus nigra do not exhibit cytotoxic activity on cells, they play a significant role in antiviral activity. As a consequence of our investigation, the cytotoxic and antiviral properties of Laurus nobilis, H. perforatum, and S. nigra extracts were found remarkable and the potential of these extracts was demonstrated.
  • Küçük Resim Yok
    Öğe
    Chemical composition and biological activities of propolis samples from different geographical regions of Turkey
    (Elsevier, 2021) Sarikahya, Nazli Boke; Goren, Ahmet C.; Okkali, Gaye Sumer; Coven, Furkan Ozan; Orman, Batuhan; Kirci, Damla; Yucel, Banu
    Propolis is a natural product with many biological activities, and it has been used in many industrial areas, such as pharmaceuticals, food and cosmetics. Thus, in this study, we aim to evaluate the chemical content, antioxidant, antiviral activities, cytotoxicity and the iNOS of propolis samples collected from 39 different regions in Turkey. Phenolic, flavonoid and triterpene profiles were determined using the LC-HRMS method. The main phenolic compounds identified were flavonoids, including diosmetin, rhamnocitrin, isosakuranetin, naringenin, chrysin, 3-O-methyl-quercetin and acacetin, the main phenolic acid was found as caffeic acid. The major triterpene compounds were also detected as oleanolic acid and hederagenin. The composition of volatile compounds in the propolis samples was examined using GC-MS. alpha-Pinene, beta-pinene and limonene were found as the main compounds in most of the samples. Cytotoxic activities against MDA-MB-231, PC-3, A549, HeLa and a nontumor cell line HEK293 were performed using the MIT method. The iNOS was also determined using LPS induced RAW 264.7 macrophage cells. Pmpolis samples exhibited significant cytotoxic and nitric oxide inhibition activities with a significant range of IC50 values. The total antioxidant/phenolic capacities in TR equivalents of pmpolis samples were assayed by DPPH, CUPRAC, FRAP, and Folin methods. The total phenolics content in the studied pmpolis extracts were found as between 0.96 and 13.53 mmol TR/g. TAC of propolis samples range with respect to the CUPRAC and FRAP methods were found from 0.71 to 8.24 and 0.21 to 2.27 mmol TR/g, respectively. Most of the propolis extracts exhibited more than 80 % DPPH scavenging activities, almost similar or higher than the positive controls. Antiviral activities of the samples were measured as virucidal activity by in ovo. All propolis samples promise for the significant inhibition of avian coronavirus. To our knowledge, this study is the first detailed study in the literature that includes the chemical content and biological activities of Turkish pmpolis collected from different geographical regions.
  • Küçük Resim Yok
    Öğe
    Evaluation of adjuvant activity of Astragaloside VII and its combination with different immunostimulating agents in Newcastle Disease vaccine
    (Academic Press Ltd- Elsevier Science Ltd, 2021) Yakubogullari, Nilgun; Coven, Furkan Ozan; Cebi, Nusin; Coven, Fethiye; Coven, Nejdet; Genc, Rukan; Bedir, Erdal
    Astragaloside VII (AST-VII), a major cycloartane saponin isolated from Turkish Astragalus species, turned out to be one of the most active metabolites demonstrating Th1/Th2 balanced immune response. As Quillaja saponins are extensively used in adjuvant systems, this study made an attempt to improve AST-VII based adjuvant systems by using different immunostimulatory/delivery agents (monophosphoryllipid A (MPL), Astragalus polysaccharide (APS) and squalene) and to induce cellular and humoral immune response against a viral vaccine. For this purpose, Newcastle Disease vaccine (NDV) was chosen as a model vaccine. Swiss albino mice were immunized subcutaneously with LaSota vaccines in the presence/absence of AST-VII or developed adjuvant systems. AST-VII administration both in live/inactivated LaSota vaccines induced neutralizing and NDV specific IgG, IgG1 and IgG2b antibodies response as well as IL-2 and IL-4 production. APS based delivery systems enhanced the production of neutralizing antibody and the minor augmentation of IFN-? and IL-2 levels. Squalene emulsion (SE) alone or combined with AST-VII were effective in NDV restimulated splenocyte proliferation. As a conclusion, AST-VII and AST-VII containing adjuvant systems demonstrated Th1/Th2 balanced antibody and cellular immune responses in NDV vaccines. Thus, these systems could be developed as vaccine adjuvants in viral vaccines as alternative to saponin-based adjuvants.
  • Küçük Resim Yok
    Öğe
    Evaluation of in ovo antiviral activities of different plant extracts and bioactive molecules with immunomodulatory properties
    (Wiley, 2021) Coven, Furkan Ozan; Gur, Seray; Uyar, Ece; Alsakini, Karrar Ali Mohammed Hasan; Bozoglan, Feyruz; Karabey, Fatih; Coven, Fethiye
    [No Abstract Available]
  • Küçük Resim Yok
    Öğe
    LC-HRMS Based Approach for Identification and Quantification Analysis of Chemical Constituents of Sea Cucumbers from Aegean Sea - Their Cytotoxic and Antiviral Potentials
    (Acg Publications, 2022) Sarikahya, Nazli Boke; Okkali, Gaye Sumer; Gunay, Deniz; Goren, Ahmet C.; Coven, Furkan Ozan; Nalbantsoy, Ayse
    There are nearly 1200 species of sea cucumber in the world's seas. Among these creatures included in the Holothuroidea class, 37 species show distribution in the Mediterranean and Aegean Sea. The purpose of this study is to determine the chemical content and biological potent of five sea cucumber species, Holothuria tubulosa, Holothuria poli, Holothuria mammata, Holothuria sanctori and Stichopus regalis which were collected from the Aegean Sea. The detailed flavonoid, phenolic and triterpene contents were determined by LCHR/MS. Cytotoxic activities against several cancer cell lines, MDA-MB-231, PC-3, A549, PANC-1, HEPG2 and a healthy cell line CCD-34LU were performed by MTT method. Antiviral activities of the samples were measured as virucidal activity against avian coronavirus by in ovo. According to the results of LC-HRMS analysis, H. sanctori, H. poli and S. regalis had the richest chemical content diversity in terms of examined triterpene compounds. Fumaric acid was detected as the most abundant substance in all sea cucumber species. H. tubulosa had a highly toxic effect on all the tested cells. The best cytotoxic activity on A549 cells was seen in H. mammata, H. sanctori and H. poli. H. sanctori also showed a significant toxic effect against PANC-1, MDA-MB-231, HepG2 and A549 cells, whereas the IC50 value in CCD-34LU cells was above 50 mu g/mL for this sample. The n-butanol extracts of sea cucumber species reduced hemagglutination (HA) virus titer between 1-fold to 4-fold in log2-based at all tested concentrations. The best inhibited virus HA titer results were found in H. tubulosa at 5 mu g/g. According to these results we have obtained, the extracts of sea cucumbers may be used in many fields such as medicine, food, cosmetics in the future. This study is also very important in terms of being a guide for all studies on the use, processing and production of sea cucumbers and detailed isolation and purification studies on sea cucumber species from Turkey.