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Öğe Allometric Growth in Hatchery-Reared Gilthead Seabream(Taylor & Francis Inc, 2009) Coban, Deniz; Kamaci, H. Okan; Suzer, Cueneyt; Saka, Sahin; Firat, KuersatMorphological development and allometric growth were investigated in the gilthead scabream Sparus aurata during larval development until the end of the weaning period on day 41. Fertilized eggs had a diameter of 1.01 +/- 0.02 mm (mean +/- SE) and a lipid vesicle diameter of 0.227 +/- 0.021 mm. Mean total length (TL) was 2.68 +/- 0.04 mm for newly hatched larvae and 3.57 +/- 0.09 mm at the onset of feeding (4 d after hatching [DAH]). At 10 DAH, initial swim bladder inflation occurred, mean TL was 4.19 +/- 0.08 mm, and notochord length (NI.) was 4.00 +/- 0.07 mm. At 16 DAH, the swim bladder became more elongated, TL was 5.13 +/- 0.09 mm, and NL was 4.94 +/- 0.07 mm. Notochord flexion occurred at 6.46 +/- 0.11 mm TL on 23 DAH, and the complement of the flexion occurred at 8.67 +/- 0.109 mm TL on 35 DAH. At the end of the weaning period (at 41 DAH). larvae were 10.85 +/- 0.116 mm TL. The majority of all allometric changes front the inflection point were expressed during the larval stage and integrated with the metamorphosis stage. Inflections in body proportion changes occurred mainly at 4.19 and 7.04 mm TL. corresponding to initial swim bladder inflation and the notochord flexion Period, respectively.Öğe Daily microgrowth increments in Otoliths of common dentex (Dentex dentex Linneaus, 1758) larvae reared in culture conditions(Scientific Technical Research Council Turkey-Tubitak, 2006) Metin, Gulner; Saka, Sahin; Firat, Kursat; Coban, DenizDaily microgrowth was determined in the otoliths of common dentex (Dentex dentex, Linneaus, 1758) larvae reared in 24 h illumination culture conditions. Microgrowth in otoliths was measured from the first mouth-opening stage of larvae on day 3 to the end of the larval stage on day 32. The sagitta and lapillus were present and were equal in size in newly hatched larvae, but the sagitta grew faster than the lapillus during further larval development. The first size increments in otoliths were observed at 4.25-4.99 mm total length of larvae (day 11). Structural development of the rostrum edge occurred on the sagitta on day 25. A polynomial relationship was found in daily lapillus growth and daily sagitta growth. This relationship was more pronounced daily lapillus growth compared to daily sagitta growth (P < 0.05). A strong positive allometry was found between sagitta and lapillus growth (r = 0.89) and between total length and lapillus growth (r = 0.98). Overall, the results obtained until the metamorphosis stage showed that there is a positive allometric relationship between total length and daily growth increments in otoliths of this species.Öğe Development of the Gastrointestinal Tract in Sharpsnout Sea Bream (Diplodus puntazzo) Larvae: Histological and Enzymatic Ontogeny(Medwell Online, 2009) Kamaci, H. Okan; Coban, Deniz; Suzer, Cuneyt; Saka, Sahin; Firat, KursatDigestive system ontogeny of the sharpsnout sea bream (Diplodus puntazzo L., 1758) was detected from hatching until 40 Days After Hatching (DAH) using histological techniques and enzymatic assays. At hatching, total length of larvae measured at 2.8 +/- 0.34 mm and it was determined on 40 DAH as 11.84 +/- 1.76 mm. At mouth opening (3 DAH), larvae almost absorbed their yolk-sac reserves. An incipient stomach could be distinguished at 2 DAH. The first evidence of gastric glands development was detected at 28 DAH, increasing in number and size by 33-40 DAH. In the fundic region, gastric gland determined as strogly and mucusal acinar cell accumulates at 30 DAH and the intestinal mucous cells appeared and developed between at 10-28 DAH. Then, functional stomach formation was detected for the first time on 32 DAH supported with sudden increase on activity of acid protease. In terms of digestive protease activity, alkaline protease was detected as early as hatching and also suddenly increased after starting of exogenous feeding. Developmental pattern for alkaline protease demonstrated constant enhancement, until metamorphosis at 25 DAH. After this date, this activity slowly decreased until end of the experiment. In contrast to this, acidic protease activity exhibited relatively lower activities during the early stages. Afterwards, concurrently with the formation of functional stomach at 32 DAH, acid protease activity suddenly >3-fold enhanced and continued to increase until end of the experiment. It is concluded that organogenesis of alimentary tract and ontogeny of digestive proteases in D. puntazzo larvae followed the same general pattern that most Sparidae species.Öğe Development of the Gastrointestinal Tract in Sharpsnout Sea Bream (Diplodus puntazzo) Larvae: Histological and Enzymatic Ontogeny(Medwell Online, 2009) Kamaci, H. Okan; Coban, Deniz; Suzer, Cuneyt; Saka, Sahin; Firat, KursatDigestive system ontogeny of the sharpsnout sea bream (Diplodus puntazzo L., 1758) was detected from hatching until 40 Days After Hatching (DAH) using histological techniques and enzymatic assays. At hatching, total length of larvae measured at 2.8 +/- 0.34 mm and it was determined on 40 DAH as 11.84 +/- 1.76 mm. At mouth opening (3 DAH), larvae almost absorbed their yolk-sac reserves. An incipient stomach could be distinguished at 2 DAH. The first evidence of gastric glands development was detected at 28 DAH, increasing in number and size by 33-40 DAH. In the fundic region, gastric gland determined as strogly and mucusal acinar cell accumulates at 30 DAH and the intestinal mucous cells appeared and developed between at 10-28 DAH. Then, functional stomach formation was detected for the first time on 32 DAH supported with sudden increase on activity of acid protease. In terms of digestive protease activity, alkaline protease was detected as early as hatching and also suddenly increased after starting of exogenous feeding. Developmental pattern for alkaline protease demonstrated constant enhancement, until metamorphosis at 25 DAH. After this date, this activity slowly decreased until end of the experiment. In contrast to this, acidic protease activity exhibited relatively lower activities during the early stages. Afterwards, concurrently with the formation of functional stomach at 32 DAH, acid protease activity suddenly >3-fold enhanced and continued to increase until end of the experiment. It is concluded that organogenesis of alimentary tract and ontogeny of digestive proteases in D. puntazzo larvae followed the same general pattern that most Sparidae species.Öğe Digestive enzyme activities in larvae of sharpsnout seabream (Diplodus puntazzo)(Elsevier Science Inc, 2007) Suzer, Cueneyt; Aktulun, Sevim; Coban, Deniz; Kamaci, H. Okan; Saka, Sahin; Firat, Kuersat; AlpbaZ, AtillaThe ontogenesis and specific activities of pancreatic and intestinal enzymes were investigated in sharpsnout sea bream, Diplodus puntazzo, during larval development until the end of weaning on day 50. The green-water technique was carried out for larval rearing in triplicate. Trypsin was first detected as early as hatching and sharply increased related to age and exogenous feeding until day 25, but a sharp decrease was observed towards the end of the experiment. Amylase was determined 2 days after hatching (DAH) and sharply increased to 10 DAH. Afterwards, slight decreases were found between 10 and 20 DAH and then slow alterations were continued until end of the experiment. Lipase was measured for the first time on day 4, and then slight increase was found to 25 DAH. After this date, slow variations were maintained until end of the experiment. Pepsin was firstly assayed 32 DAH related with stomach formation and sharply increased to 40 DAH. Then it was fluctuated until end of the experiment. Enzymes of brush border membranes, alkaline phosphatase and aminopeptidase N, showed similar pattern on specific activities during the first 10 days. Thereafter, while specific activity of alkaline phosphatase slightly decreased to 15 DAH and fluctuated until 20 DAH, aminopeptidase N activity slowly declined to 20 DAH. Afterwards, activity of alkaline phosphatase and aminopeptidase N were sharply increased to 30 DAH, showing maturation of the intestinal digestive process and also these activities continued to slight increase until end of the experiment. The specific activity of cytosolic peptidase, leucine-alanine peptidase sharply increased to on day 8, then suddenly declined to 12 DAH and further decreased until 20 DAH. After this date, in contrast to enzymes of brush border membranes, it sharply decreased to 25 DAH and continued to gradually decline until the end of the experiment. These converse expressions were indicative of a maturation of enterocytes and the transition to an adult mode of digestion. (C) 2007 Elsevier Inc. All tights reserved.Öğe Digestive enzyme activity of the red porgy (Pagrus pagrus, L.) during larval development under culture conditions(Wiley-Blackwell, 2007) Suzer, Cueneyt; Kamaci, H. Okan; Coban, Deniz; Saka, Sahin; Firat, Kuersat; Ozkara, Banu; Ozkara, AlaattinThe ontogenic development of the main digestive enzymes (proteases, amylase and lipase) in the red porgy, Pagrus pagrus, larvae was assayed during the larval development. The green water technique was carried out for larval rearing and whole-body homogenates were used for enzymatic assays in triplicate. Significant alterations in specific activities of all digestive enzymes measured during the period of this study were mostly related to metamorphosis and weaning. Trypsin- and chymotrypsin-specific activities were first detected on day 3, together with opening of the mouth, and slightly increased until 25 days after hatching (DAH). After this period, the specific activities of these enzymes slightly decreased. Pepsin was first detected on day 28, concurrent with stomach formation, and a sharp increase was observed until 30 DAH. A slight decrease was measured from this date until the end of the experiment. Both amylase and lipase were measured for the first time on days 2 and 4 respectively, and the specific activities of these enzymes showed similar patterns during the first week of the study. Then, slight variations were observed until 30 DAH and while lipase-specific activity declined, an increase in the specific activity of amylase was found until the end of the experiment. It is concluded that the variations observed in the specific activity of digestive enzymes were related to either metamorphosis, such as the formation of the stomach (28 DAH), or to changes in food composition. The profile of the developmental pattern of the main digestive enzymes detected in P. pagrus is similar to that described for other Sparid species.Öğe Early Development of Cephalic Skeleton in Hatchery-Reared Gilthead Seabream, Sparus aurata(Central Fisheries Research Inst, 2008) Saka, Sahin; Coban, Deniz; Kamaci, H. Okan; Suezer, Cueneyt; Firat, KuersatThe development of the cephalic skeleton in gilthead seabream, Sparus aurata (L. 1758), larvae was examined from 1 to 41 days post-hatching during the early ontogeny tinder intensive culture conditions. At hatching, S. aurata larvae had 2.68 mm total length (TL) and were devoid of any cephalic elements. At 2.85 mm TL, the trabecular bars and the ethmoid plate appeared. Development of jaws started during the yolk-sac stage. Teeth first appeared at larval stage (7.78 mm TL) and gill filaments formed at size ranging between 8.05 and 8.9 mm TL. Finally, the dentary, maxillary and Mcckel's cartilage began to ossify at 9.20 mm TL. It is Concluded that cartilaginous elements and structures could be related with vital functions in S. aurata larvae. Also, onset time of skull was found different although same developmental pattern of skull has been described for most Sparidae species to date.Öğe Early Morphological Development and Allometric Growth Patterns in Hatchery-Reared Red Porgy (Pagrus pagrus)(Central Fisheries Research Inst, 2014) Yildirim, Sukru; Coban, Deniz; Suzer, Cuneyt; Kirim, Birsen; Firat, Kursat; Saka, SahinMorphological development and allometric growth were investigated in the red porgy, Pagrus pagrus, during larval development until the end of weaning at 46 days after hatching (DAH). Total length (TL) of newly hatched larvae was 2.77 +/- 0.09 mm (mean +/- SE). Onset of exogenous feeding occurred at 4 DAH (3.74 +/- 0.13 mm TL). Initial swimbladder inflation occured at 10 DAH (4.22 +/- 0.18 mm TL) and post-inflation became more elongate at 16 DAH (4.95 +/- 0.26 mm TL). Notochord flexion occurred between 22 DAH (5.56 +/- 0.43 mm TL) and 34 DAH (8.22 +/- 0.63 mm TL). At the end of the weaning at 46 DAH, TL of larvae was 15.81 +/- 1.36 mm According to individual measurements of a total of 1026 larvae of 18 body parameters for allometric growth, in 19 of 35 respective regression equations, the allometry coefficients were positive, while coefficients of 7 equations showed negative allometry. The majority of all allometric changes from inflection point were expressed during the larval stage and were integrated with the metamorphosis stage. Inflections in body proportion changes occurred mainly at TL values of 3.74, 4.22 and 5.56 mm, coinciding with mouth opening, initial swimbladder inflation and flexion of notochord, respectively.Öğe Early Weaning of Sea Bass (D. labrax) Larvae: Effects on Growth Performance and Digestive Enzyme Activities(Central Fisheries Research Inst, 2011) Suzer, Cuneyt; Kamaci, H. Okan; Coban, Deniz; Saka, Sahin; Firat, Kursat; Karacaoglan, ArifIn this study, effects of early weaning of sea bass (D. labrax) by microparticulate food on larval development and digestive enzyme activities, acid protease and alkaline protease were investigated until day 40. Larval rearing was conducted in closed sea water systems. Experiments were triplicated on 3 different weaning protocol that introduced on day 15 (MD 15), day 20 (MD20) and day 25 (MD25) and also no microparticulate diet (MD) distributed in the control group. At the end of the experiment at 40-day, larval growth was calculated and the best values were obtained from MD25. Statistical differences among groups were not important (P>0.05) whereas MD15 was found different than the other groups (P<0.05). Also, survival rates were calculated as 3.6%, 14.7%, 43.6%, and 34.1% for the experimental groups, respectively. MD15 and MD20 groups were found different than the other groups (P<0.05). Nevertheless, activity of alkaline protease was detected synchronously by mouth opening and exogenous feeding and also increased by larval development. According to histological analysis, pancreas was observed by mouth opening and similarly developed with increasing of larval age. Alkaline protease activity changed by nutritional composition of feed and especially shifting of feed affected the enzymatic activity. There was no differences among the experimental groups (P>0.05) while control group was found statistically different (P<0.05). According to histological analysis, gastric glands and formation of functional stomach were observed on day 24-25 in all experimental groups. Acid protease activity firstly detected at 25 DAH. Control group was found different than the other groups (P<0.05). Finally, in terms of both growth parameters and survival rate and digestive enzyme activities it is clearly concluded that MD supplementation could be started after 25 DAH in D. lahrax larviculture.Öğe Effect of Some Morphometric Characteristics on Egg Quality in Common Dentex, Dentex dentex (Linnaeus, 1758)(Central Fisheries Research Inst, 2011) Coban, Deniz; Kamaci, H. Okan; Suzer, Cuneyt; Yildirim, Sukru; Arda, Gokhan; Korkut, A. Yildirim; Saka, Sahin; Firat, KursatIn this study, egg quality determination of common dentex (Dentex dentex) was investigated by using egg, yolk and oil globule diameter from the first division to the hatching, during natural and photoperiodic manipulation of spawning time. In the viable, floating eggs, the minimal and maximal diameter of the egg, yolk and oil globule were measured. Based on these measurements several parameters were calculated describing the shape of the egg, yolk and oil globule and the size relationship between these compartments. Hatching rate was calculated as 92% in natural spawning time (NST) and 61% photoperiodic manipulation of spawning time (PMST). Also, average egg diameter was measured as 0.949 +/- 0.038 mm in NST and 0.911 +/- 0.014 mm in PMST. In NST, egg diameter and yolk diameter of the eggs were significantly bigger than eggs in PMST (P<0.05). The morphometric parameters of oil globule could be accepted as an indicator for description of quality criteria of eggs.Öğe Effects of Illumination Intensity on Growth Parameters and Swim Bladder Development in Common dentex (Dentex dentex, L.) Larvae(Central Fisheries Research Inst, 2013) Yildirim, Sukru; Suzer, Cuneyt; Coban, Deniz; Kamaci, H. Okan; Firat, Kursat; Saka, SahinThe influences of three different light intensities were investigated on growth of common dentex (Dentex dentex) during larval period (0-35 day). Three different illumination levels were compared that 10 lx as group A, 30 lx described group B and 100 lx named group C in triplicates, respectively. Larvae were cultured in a recirculating aquaculture system using a green water technique. The longest total length and the heaviest weight were found as 18.43 +/- 1.9 mm and 38.48 +/- 5.1 mg in group B with 30 lx light intensity. Same parameters were 18.03 +/- 2.5 mm and 36.72 +/- 4.8 mg for group C with 100 lx light intensity; 16.23 +/- 1.7 mm and 28.53 +/- 4.2 mg for group A with 10 lx light intensity. In terms of total length and weight, there were significant differences in group A (P<0.05), however, differences were not detected between B and C groups (P>0.05). Also, swim bladder inflation rates were calculated as 88.3 +/- 2.7, 92.1 +/- 1.6 and 91.4 +/- 1.3% for groups A, B and C, respectively and did not presented significant differences among groups (P>0.05). In addition, survival rates were 8.8 +/- 3.1, 23.5 +/- 2.5 and 19.9 +/- 2.8%, respectively. While not significant differences were found between group B and C (P>0.05), but group A presented significantly difference compared to other groups (P<0.05). As a result, it is thought that illumination effected husbandry parameters were more affirmative with 30 lx light intensity in Dentex dentex under culture conditions.Öğe Effects of Probiotic (Bacillus sp.) Supplementation during Larval Development of Gilthead Sea Bream (Sparus aurata, L.)(Central Fisheries Research Inst, 2013) Arig, Nihan; Suzer, Cuneyt; Gokvardar, Alize; Basaran, Fatih; Coban, Deniz; Yildirim, Sukru; Kamaci, H. Okan; Firat, Kursat; Saka, SahinIn this study, the effects of administration of commercial probiotic, Bacillus sp., were examined on growth parameters and digestive proteases (alkaline and acid proteases) activities in gilthead sea bream, Sparus aurata, during larval development until on day 40. Probiotics were supplemented in triplicate from on day 3 coinciding with starting of exogenous feeding. In the experimental group, probiotic was introduced to rotifer and tank water until 20 days after hatching. Also, control group did not receive probiotic. In experimental group, the specific activities of alkaline protease was significantly higher (p<0.05) in larvae compared to control group. Acid protease activity was not effected by probiotic supplementation however, it was relatively higher in experimental group than the control (p>0.05). Additionally, no significant differences were found in both survival (8.5% higher) and specific growth rate (0.7% higher) larvae of S. aurata that had probiotic supplemented by rotifer with water as compared to controls (p>0.05). Consequently, specific activities of alkaline and acid protease could be significantly increased by the administration of Bacillus sp. to live food with water the and therefore; this method would be more effective for obtaining of relatively better growth parameters and nutritional condition in S. aurata larvae.Öğe External morphology of European seabass (Dicentrarchus labrax) related to sexual dimorphism(Tubitak Scientific & Technical Research Council Turkey, 2011) Coban, Deniz; Yildirim, Sukru; Kamaci, H. Okan; Suzer, Cuneyt; Saka, Sahin; Firat, KursatThe relationships among 15 morphometric measurements in 219 aliquots of European seabass (Dicentrarchus labrax) were examined with respect to sexual dimorphism. The ratio of females to males was determined as 1:5.1 and gonadosomatic indices (%) were found for males (2.13 +/- 0.86) and females (3.63 +/- 1.18). There were 4 morphometric characteristics that differed between genders: ventral margin of cleithrum (VMC), postanal fin length (post-AFL), postdorsal fin length (post-DFL), and preanal fin length (pre-AFL). Although there were significant differences between morphometric characteristics (post-AFL, post-DFL, pre-AFL) and total length, no significant differences were found in the VMC. These average ratios were determined as pre-AFL to TL 0.61 female, 0.71 male; post-AFL to TL 0.72 female, 0.84 male; post-DFL to TL 0.68 female, 0.81 male, respectively. The aim of this study was to investigate if sexual differences could affect external morphology in cultured seabass and, additionally, to determine sexual dimorphism related to morphological differences during the maturation and spawning seasons in this species.Öğe The First Data on the Saddleback Syndrome in Cultured Gilthead Sea Bream (Sparus aurata L.) by MIP-MPR Method(Medwell Online, 2009) Korkut, Ali Y.; Kamaci, H. Okan; Coban, Deniz; Suzer, CueneytÖğe The First Data on the Saddleback Syndrome in Cultured Gilthead Sea Bream (Sparus aurata L.) by MIP-MPR Method(Medwell Online, 2009) Korkut, Ali Y.; Kamaci, H. Okan; Coban, Deniz; Suzer, CueneytÖğe Functional changes in digestive enzyme activities of meagre (Argyrosomus regius; Asso, 1801) during early ontogeny(Springer, 2013) Suzer, Cuneyt; Kamaci, H. Okan; Coban, Deniz; Yildirim, Sukru; Firat, Kursat; Saka, SahinThe ontogenesis of main pancreatic and intestinal enzymes was investigated in the recent promising Mediterranean candidate species of meagre, Argyrosomus regius, during larval development until 40 days after hatching (DAH). The green-water technique was carried out for larval rearing. Whole-body homogenates were used for enzymatic analysis in larvae younger than 15 DAH; after this date, older larvae were dissected into two segments as pancreatic and intestinal segment. Trypsin was detected as early as hatching and sharply increased concurrently with age and exogenous feeding 15 DAH, but constant decline was observed until the end of experiment. Amylase was determined at 2 DAH and sharply increased 10 DAH. Then, slight decreases were found between 10 and 15 DAH, and then slow alterations were continued until the end of the experiment. Lipase was firstly measured on day 3; then, sudden decline was observed between 20 and 25 DAH. After this date, slow fluctuations were maintained until the end of the experiment. Pepsin was firstly assayed 15 DAH related to gastric gland secretion and sharply increased 30 DAH. Then, it slowly varied until end of the experiment. Enzymes of brush border membranes, alkaline phosphatase and aminopeptidase N showed similar pattern on specific activities during the first 10 days. Thereafter, while specific activity of alkaline phosphatase slightly decreased 15 and fluctuated until 20 DAH, aminopeptidase N activity slowly increased 20 DAH. Then, activity of alkaline phosphatase and aminopeptidase N constantly increased 30 DAH, indicating maturation of the intestinal digestive process, and also, these activities continued to slowly increase until the end of the experiment. The specific activity of cytosolic peptidase, leucine-alanine peptidase, smoothly increased on day 8, then fluctuated until 15 DAH. After this date, in contrast to enzymes of brush border membranes, it sharply decreased 25 DAH and continued to gradually decline until the end of the experiment. These converse expressions were indicative of a maturation of enterocytes and the transition of an adult mode of digestion.Öğe Impact of probiotic Bacillus sp. dietary supplementation on pancreatic and intestinal activities in seabream Sparus aurata(Oxford University Press, 2024) Yildirim, Sukru; Suzer, Cuneyt; Firat, Kursat; Saka, Sahin; Hekimoglu, Muge; Coban, Deniz; Korkut, Ali Yildirim; Kose, Ibrahim; Antepli, Onurkan; Gokvardar, AlizeThe main objective of this study was to investigate the effects of commercial probiotic Bacillus sp. supplementation on seabream Sparus aurata larviculture under culture conditions. In this context, Bacillus was supplemented via rotifer feeding and water and its effects on pancreatic and intestinal enzyme activities as well as aquaculture parameters were evaluated during early life development. In the experimental group, as probiotic three Bacillus sp. spores were introduced via rotifer and larval culture tanks, while the larvae in control group did not feed any probiotic supplementation. At the end of the experiment on 40 days after hatching, the probiotic-supplemented group exhibited better growth performance and there were statistically differences in between groups of probiotic-treated and control regarding growth parameters (P < 0.01), despite insignificant survival rate (P > 0.05). In terms of enzymatic expressions, S. aurata larvae receiving probiotic supplementation through rotifers demonstrated noteworthy (P < 0.05) enhancements in specific activities of pancreatic and intestinal enzymes, except for amylase (P > 0.05), when compared to the control group. It is concluded that the administration of Bacillus sp. as probiotic bacteria through rotifer supplementation and water intake demonstrates significant positive impacts on both growth parameters and specific activities of main pancreatic and intestinal enzymes of seabream larvae.Öğe Morphological Development and Allometric Growth of Sharpsnout Seabream (Diplodus puntazzo) Larvae(Central Fisheries Research Inst, 2012) Coban, Deniz; Suzer, Cuneyt; Yildirim, Sukru; Saka, Sahin; Firat, KursatIn this study, morphological development and allometric growth were investigated in the sharpsnout seabream, Diplodus puntazzo, during larval development until the end of the weaning on day 42. Average total length (TL) of newly hatched larvae was 2.91 +/- 0.11 mm and it was 3.35 +/- 0.13 mm TL at the onset of feeding at 4 days after hatching (DAH). Initial swimbladder inflation occurred at 10 DAH (5.11 +/- 0.45 mm TL) and post-inflation became more elongate at 15 DAH (5.95 +/- 0.43 mm TL). The notochord flexion occurred between 5.95 +/- 0.43 mm TL at 15 DAH and 7.98 +/- 0.72 mm TL on 24 DAH. At the end of weaning, larvae were 16.03 +/- 1.74 mm TL at 42 DAH. The majority of all allometric changes from inflection point were integrated with the larval and the metamorphosis stage. Inflections in body proportion changes occurred mainly at 5.12, 5.95 and 7.98 mm TL, corresponding to initial swimbladder inflation and flexion period of notochord, respectively.Öğe Morphometric comparison of cultured and lagoon caught gilthead seabream (Sparus aurata L. 1758)(Tubitak Scientific & Technical Research Council Turkey, 2008) Coban, Deniz; Saka, Sahin; Firat, KuersatShape differences were analyzed in cultured and lagoon (Homa Lagoon in Izmir Bay, Turkey; 38 degrees 33'23'N-26 degrees 50'42'E) caught sea bream, Sparus aurata. Specimens in group A (n = 35, 19.71 +/- 1.43 cm TL) were collected from nature, while cultured fish in group B (n = 32, 15.12 +/- 0.44 cm TL) were propagated in a private hatchery. In order to reveal shape differences in the fish, 13 landmarks was determined in specimens of both groups and the computer programs Tpsdig, Tpsrelw, Tpssplin, and Morpheus were used to demonstrate differences in the selected landmarks. No significant differences were found in terms of geometrical morphometry between lagoon caught and cultured fish (P > 0.05). It is thought that the similar shape formation in specimens of both groups was related to similarities in conditions between culture and lagoon environments due to feeding and stocking.Öğe Organogenesis and Enzymatic Functionality of Exocrine Pancreas in Cultured Gilthead Sea Bream (Sparus aurata) Larvae(Medwell Online, 2009) Kamaci, H. Okan; Suzer, Cuneyt; Coban, Deniz; Firat, Kursat; Saka, SahinThe ontogeny of the exocrine pancreas was studied histological with the expression of digestive protease, trypsin, activity in gilthead sea bream, Sparus aurata, L. larvae from hatching to 30 days after Hatching (DAH). The pancreas was identified as a well developed differentiated cells located dorsal and slightly posterior to the liver at 3 DAH. Incipient pancreas with exocrine polyhedral cells could be observed and first zymogen granules detected at this period. Until the larval metamorphosis, the pancreas became diffuse, spreading throughout the mesentery enclosure the stomach, the upper intestine and the pyloric caeca. The specific activity of trypsin (48.35+/-4.3 mU/mg/protein) and chymotrypsin (205.38+/-46.2 mU/mg/protein) was determined as early as after hatching at 2.27+/-0.14 mm Total Length (TL) of larvae and increased immediately during the following days especially, after exogenous feeding. The highest trypsin and chymotrypsin activity was detected at 25 DAH as 11 8.26+/-10.23 mU/mg/protein and 1067.53+/-118.42 mU/mg/protein, respectively that concurrently detected with Artemia metanauplii introduction. It is concluded that exocrine pancreas organogenesis is the main critical step of the zymogen granules and specific activities digestive proteases, trypsin and chymotrypsin, were present as early as after hatching and continuously increasing with larval period of Sparus aurata.
