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Öğe Analysis of expressed genes in normal and tumoral mammary gland tissue of the terrier dog [Terrier köpeklerinin normal ve tümörlü meme bezi dokusunda eksprese edilen genlerin birincil analizi](Veteriner Fakultesi Dergisi, 2018) Ozdemir Ozgenturk N.; Omeroglu Ulu Z.; Ulu S.; Celik M.; Tellioglu B.; Yildirim F.; Akis Akad I.; Gurel A.; Un C.; Oztabak K.O.Mammary gland tumor is the most common type of tumor in female dogs. Data on genes that are involved in tumorigenesis and mechanism of tumor development are insufficient. Comparative studies have been conducted in order to see if tumorigenesis studies in the dog could be a model for human mammary gland tumors. In this study, we constructed two different cDNA libraries from mammary tissue, which were collected from a normal mammary tissue of a healthy Terrier dog and a tumoral mammary tissue of a sick dog. A total 2304 colonies which are randomly picked out from the two libraries were sequenced for developing a dog mammary gland ESTs collection. Raw EST data were analyzed with Phred/Phrap programs and readable EST sequences were assembled with the CAP3 program. All of EST sequences were grouped into 45 contig and 2203 singletons. Putative functions of all unique sequences were designated by NCBI BLAST based on gene homology and annotated by BLAST2GO. The results of this study are a very valuable resource for functional genome studies of the dogs. © 2018, Veteriner Fakultesi Dergisi. All rights reserved.Öğe Bacterial agents causing meningitis during 2013–2014 in Turkey: A multi-center hospital-based prospective surveillance study(Taylor and Francis Inc., 2016) Ceyhan M.; Ozsurekci Y.; Gürler N.; Karadag Oncel E.; Camcioglu Y.; Salman N.; Celik M.; Emiroglu M.K.; Akin F.; Tezer H.; Parlakay A.O.; Tuygun N.; Tamburaci D.; Dinleyici E.C.; Karbuz A.; Uluca Ü.; Alhan E.; Çay Ü.; Kurugol Z.; Hatipoğlu N.; Şiraneci R.; İnce T.; Sensoy G.; Belet N.; Coskun E.; Yilmaz F.; Hacimustafaoglu M.; Celebi S.; Celik Ü.; Ozen M.; Akaslan A.; Devrim İ.; Kuyucu N.; Öz F.; Bozdemir S.E.; Kara A.This is an observational epidemiological study to describe causes of bacterial meningitis among persons between 1 month and 18 y of age who are hospitalized with suspected bacterial meningitis in 7 Turkish regions. covering 32% of the entire population of Turkey. We present here the results from 2013 and 2014. A clinical case with meningitis was defined according to followings: any sign of meningitis including fever, vomiting, headache, and meningeal irritation in children above one year of age and fever without any documented source, impaired consciousness, prostration and seizures in those < 1 y of age. Single tube multiplex PCR assay was performed for the simultaneous identification of bacterial agents. The specific gene targets were ctrA, bex, and ply for N. meningitidis, Hib, and S. pneumoniae, respectively. PCR positive samples were recorded as laboratory-confirmed acute bacterial meningitis. A total of 665 children were hospitalized for suspected acute meningitis. The annual incidences of acute laboratory-confirmed bacterial meningitis were 0.3 cases / 100,000 population in 2013 and 0.9 cases/100,000 in 2014. Of the 94 diagnosed cases of bacterial meningitis by PCR, 85 (90.4%) were meningococcal and 9 (9.6%) were pneumococcal. Hib was not detected in any of the patients. Among meningococcal meningitis, cases of serogroup Y, A, B and W-135 were 2.4% (n = 2), 3.5% (n = 3), 32.9% (n = 28), and 42.4% (n = 36). No serogroup C was detected among meningococcal cases. Successful vaccination policies for protection from bacterial meningitis are dependent on accurate determination of the etiology of bacterial meningitis. Additionally, the epidemiology of meningococcal disease is dynamic and close monitoring of serogroup distribution is comprehensively needed to assess the benefit of adding meningococcal vaccines to the routine immunization program. © 2016 Taylor & Francis.Öğe Cricopharyngeal muscle electromyography in laryngopharyngeal reflux(2005) Celik M.; Alkan Z.; Ercan I.; Ertasoglu H.; Alkim C.; Erdem L.; Turgut S.; Ertekin C.Objectives/Hypothesis: The objective was to determine whether laryngopharyngeal reflux was associated with an electrophysiological abnormality of the main part of the upper esophageal sphincter, namely, the cricopharyngeal muscle, which could be related to laryngopharyngeal reflux symptoms. Study Design: A prospective study of 20 patients with symptoms of laryngopharyngeal reflux. Methods: The diagnosis was based on clinical, laryngoscopic, and ambulatory 24-hour pH monitoring findings. Double-probe pH monitoring was performed with placement of the distal and proximal probes 5 cm above the proximal border of the lower esophageal sphincter and 15 cm proximal to the distal probe, in or just beneath the upper esophageal sphincter. Electromyographic recordings were obtained simultaneously on submental and cricopharyngeal muscles during water swallowing. The peak of the submental electromyographic amplitude and its duration was measured using bipolar surface electrodes placed under the chin. The electromyographic features of the cricopharyngeal muscle were investigated by means of concentric needle electrodes inserted percutaneously both in 20 normal subjects and in 20 patients with laryngopharyngeal reflux. Results: All electrophysiological parameters were normal in all of the patients. The durations of foreburst revealed significant correlation with the number of reflux episodes and acid exposure time (P = .026 and P = .044, respectively). Conclusion: The duration of the foreburst correlating with acid exposure time and number of reflux episodes suggested that foreburst duration was extended because of the irritation caused by the acid reflux.Öğe Effectiveness of a new bioequivalent formulation of oseltamivir (Enfluvir ®) on 2010-2011 seasonal influenza viruses: An open phase IV study(2012) Ceyhan M.; Karadag Oncel E.; Badur S.; Ciblak M.A.; Alhan E.; Celik U.S.; Kurugol Z.; Saz E.U.; Ozsurekci Y.; Celik M.; Parlakay A.O.Objective: The aim of this multicenter prospective study was to evaluate the efficacy of a new bioequivalent formulation of oseltamivir for the treatment of influenza A, influenza B, and H1N1 during the 2010-2011 influenza season. Methods: We compared the symptoms and signs of 300 pediatric patients presenting to three university hospitals with an influenza-like illness between January and March 2011. Nasal swab specimens were collected from all children and tested by reverse-transcription polymerase chain reaction (RT-PCR) for influenza viruses. After randomization, half of the participants were prescribed oseltamivir, while the other half were observed conservatively. Forty patients who were followed-up for influenza prior to the study were also included in the evaluation. Results: Influenza was confirmed by RT-PCR in 129 children, 71 of whom were prescribed oseltamivir. The durations of the symptoms fever, cough, nasal congestion, and rhinorrhea were significantly shorter for patients who were treated with oseltamivir compared with untreated patients (. p<. 0.002 for all symptoms). Early initiation of oseltamivir therapy (within 48. h of the onset of symptoms) was associated with more favorable outcomes and an earlier recovery than in patients for whom treatment was delayed (beyond 48. h). Thirty-seven patients (28.7%) had H1N1, 44 (34.1%) had influenza A, 46 (35.7%) had influenza B, one (0.8%) had H1N1 plus influenza A, and one (0.8%) had influenza A plus influenza B viruses. In the comparison of the duration of symptoms according to the different virus types, a statistically significant difference was only observed in patients with influenza B who had a longer duration of cough (. p<. 0.001), nasal congestion (. p<. 0.001), and rhinorrhea (. p<. 0.001). Conclusions: Oseltamivir is an effective treatment for the management of seasonal influenza and H1N1, and should be initiated immediately without waiting for laboratory confirmation of diagnosis. © 2012 International Society for Infectious Diseases.Öğe Meningitis caused by neisseria meningitidis, hemophilus influenzae type b and streptococcus pneumoniae during 2005-2012 in Turkey: A multicenter prospective surveillance study(Landes Bioscience, 2014) Ceyhan M.; Gürler N.G.; Ozsurekci Y.; Keser M.; Aycan A.E.; Gurbuz V.; Salman N.; Camcioglu Y.; Dinleyici E.C.; Ozkan S.; Sensoy G.; Belet N.; Alhan E.; Hacimustafaoglu M.; Celebi S.; Uzun H.; Oner A.F.; Kurugol Z.; Ali M.; Aygun D.; Oncel E.K.; Celik M.; Yasa O.; Akin F.; Coşkun Y.Successful vaccination policies for protection from bacterial meningitis are dependent on determination of the etiology of bacterial meningitis. Cerebrospinal fluid (CSF) samples were obtained prospectively from children from 1 month to ? 18 years of age hospitalized with suspected meningitis, in order to determine the etiology of meningitis in Turkey. DNA evidence of Neisseria meningitidis (N. meningitidis ), Streptococcus pneumoniae ( S. pneumoniae), and Hemophilus influenzae type b (Hib) was detected using multiplex polymerase chain reaction (PCR). In total, 1452 CSF samples were evaluated and bacterial etiology was determined in 645 (44.4%) cases between 2005 and 2012; N. meningitidis was detected in 333 (51.6%), S. pneumoniae in 195 (30.2%), and Hib in 117 (18.1%) of the PCR positive samples. Of the 333 N. meningitidis positive samples 127 (38.1%) were identified as serogroup W-135, 87 (26.1%) serogroup B, 28 (8.4%) serogroup A and 3 (0.9%) serogroup Y; 88 (26.4%) were non-groupable. As vaccines against the most frequent bacterial isolates in this study are available and licensed, these results highlight the need for broad based protection against meningococcal disease in Turkey. © 2014 Taylor & Francis Group, LLC
