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REAL TIME BACTERIAL MONITORING DURING THE BIODEGRADATION OF SELECTED PAHs IN INDUSTRY-BORNE ACTIVATED SLUDGE
(Scibulcom Ltd, 2014) Vural, C.; Karabey, B.; Bayrak, D. E.; Ozdemir, G.
Selected polyaromatic hydrocarbons (PAHs) were evaluated for their degradation potentials in aerobic completely stirred tank reactor (CSTR) and bacterial monitoring was performed with using real time polymerase chain reaction (real time PCR). For this, identified bacteria were inoculated to continuous stirred tank reactor (CSTR) for degradation of selected PAHs. During the degradation process, bacterial existences and numbers were monitored with using designed primer-probe sets in real time PCR. As a mean of the ageing period, 55 days, pH was 7.5 and the dissolved oxygen (DO) was 4 mg/l. Mean of the chemical oxygen demand (COD) and mixed liquor suspended solids (MLSS) rates in effluent 282 and 219 mg/l, respectively, and sludge volume was 373 ml/l. All selected isolates have shown stable results. As a mean of 36 days of biodegradation, Acinetobacter sp., Burkholderia cepacia, Chryseobacterium sp. and Pseudomonas fluorescens were calculated as 2.3 x 10(5), 2.9 x 10(5), 1.5 x 10(6) and 3.6 x 10(7), respectively.