Yazar "Arserim S.K." seçeneğine göre listele

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    [Detection of entomopathogen nematode [EPN - sand flies (Phlebotomus tobbi)] caught in the wild in Aydin, Kuşadasi town and its assessment as a biological control agent]. [Kuşadasi, Aydin'da Dogadan Yakalanan Kum Sineklerinde (Phlebotomus tobbi) Saptanan Entomopatojen Nematod [EPN] ve Biyolojik Kontrol Ajani Olarak Kullaniminin Degerlendirilmesi.]
    (2013) Karakuş M.; Arserim S.K.; Töz S.O.; Özbel Y.
    In this study, the midgut of the sand flies investigated with direct method for the presence of parasites and other organisms. Wild sand flies collected in Kuşadasi Town-Aydin, were dissected and midgut contents were examined by light microscopy. After midgut dissection, the head and genitalia of sand fly specimens were clarified and mounted for species identification. During the study, a total of 1027 sand flies were dissected. Eight and two species belonging to Phlebotomus and Sergentomyia genera were determined, respectively. Phlebotomus tobbi was found to be most abundant species (61.34%). A third stage of infective Entomopathogen Nematode belonging to Steinernematidae family was observed in the hemocoel of one specimen of P. tobbi during the dissection process. This is the first finding related to entomopathogen nematodes found in sand flies in Turkey. In the study, the sand fly fauna was determined in Kuşadasi Town. For the control of sand flies, entomopathogenic nematodes which are not harmful for non-target organisms, can be used instead of chemical insecticides that can cause unknown damage in the environment.
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    [An epidemiological study on canine leishmaniasis (CanL) and sand flies in Northern Cyprus]. [Kuzey Kibris'ta Kanin Leishmaniasis ve Kum Sineklerinin Epidemiyolojisi.]
    (2013) Töz S.O.; Ertabaklar H.; Göçmen B.; Demir S.; Karakuş M.; Arserim S.K.; Balcioglu I.C.; Canakçi T.; Ozbel Y.
    In this study, the investigation on the prevalence of canine leishmaniasis (CanL) and sand fly species incriminated as potential vectors of leishmaniasis in the northern part of the Cyprus were aimed. This research was conducted in two periods; 2004 and 2012. Serological (IFAT and rK39) and molecular (PCR) tests were performed on 83 dog blood samples during the 2004 survey. PCR was performed using primers 13A/13B targeting kinetoplastid minicircle constant region. Genomic Nested-PCR was applied using R221/R332 and R323/333 primers for 5 clinically suspected dog samples in 2012. Sand flies were collected from the Lapithos town and Kyreniae province using CDC light traps and midgut dissection was done for the presence of Leishmania parasites during the 2012 survey. Three (3.61%) out of 83 dogs were found to be positive for CanL in 2004, while 3 out of 5 clinically suspected dogs were positive in 2012. In total 296 female sand flies were dissected and 9 species belonging to Phlebotomus and Sergentomyia genera were determined. No promastigote was found in the dissected females. The results obtained in two different periods showed that the importance and risk of canine disease are increasing in the northern Cyprus and further studies should be performed in northern Cyprus for determining the incidence of canine and human leishmaniasis.
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    Evaluation of conjunctival swab sampling in the diagnosis of canine leishmaniasis: A two-year follow-up study in Çukurova Plain, Turkey
    (Elsevier B.V., 2015) Karakuş M.; Töz S.; Ertabaklar H.; Paşa S.; Atasoy A.; Arserim S.K.; Ölgen M.K.; Ziya Alkan M.; Durrant C.; Özbel Y.
    The diagnosis of canine leishmaniasis (CanL) in symptomatic and asymptomatic dogs is a very important and problematic public health issue in Turkey. A longitudinal study was carried out on dogs in selected villages in the Çukurova Plain in Turkey, from July 2011 to June 2013, where cutaneous (CL) and visceral (VL) leishmaniasis is endemic. The study aimed to determine the prevalence of CanL and to evaluate the early diagnostic performance of the non-invasive conjunctival swab nested PCR (CS n-PCR) test in comparison with the Indirect Fluorescent Antibody Test (IFAT). The consecutive blood and CS samples from a representative number of dogs (80-100 dogs/each survey) were collected in a cohort of 6 villages located in the area. Clinical symptoms, demographic and physical features about each dog were noted and lymph node aspiration samples were obtained from selected dogs with lymphadenopathy. In four surveys during the period, a total of 338 sets (blood and CS) of samples from 206 dogs were obtained, such that 83 dogs were sampled more than once. In the cross-sectional analysis, the CanL prevalence was found to be 27.18% (between 7.14% and 39.13%) by IFAT and 41.74% (between 29.03% and 46.66%) by CS n-PCR. The isolated strains were identified as Leishmania infantum MON-1 (n = 9) and MON-98 (n = 2) by MLEE analysis. Genetic studies targeting the Hsp70 and ITS1 regions performed on 11 dog isolates also showed two clear separate groups. According to IFAT results, 24 of the 83 dogs sampled more than once showed seroconversion (n = 19) or a four-fold increase in Ab titers (n = 5), while 17 were positive in the initial screening. Forty-two dogs stayed negative during the whole period. The natural Leishmania exposure rate was detected as 31.14% in the study area. CS n-PCR only detected Leishmania infection earlier than IFAT in 8 dogs. No statistical difference was found after the analysis of demographical and physical data. The results indicated that (i) circulation of the dog population is very common in settlements in the Çukurova Plain, but the disease prevalence is high and stable, (ii) the performance of CS n-PCR for detecting Leishmania-dog contact is higher than IFAT, (iii) and some of the parasites isolated from dogs have different zymodemes and/or genotypes from previous human and sand fly isolates; suggesting the probability of two different cycles of leishmaniasis in this particular area. This hypothesis should be supported by future studies targeting vectors and reservoirs. © 2015 Elsevier B.V.
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    Fumigant toxicity of Satureja cuneifolia and Ziziphora clinopodioides essential oils on field collected sand flies (Diptera: Psychodidae: Phlebotomie)
    (Parlar Scientific Publications, 2018) Cetin H.; Ser O.; Arserim S.K.; Polnt Y.; Ozbek T.; Civil M.; Cinbilgel I.; Ozbel Y.
    Sand flies (Diptera: Psychodidae: Phlebotominae) are the vectors of Leishmania species. Since the larval control of the sand flies is very difficult and almost impossible, the fighting has mainly been carried out for the adults. In this research the fumigant toxicity of two plant essential oils obtained from Satureja cuneifolia Ten. and Ziziphora clinopodioides Lam. was evaluated against fieldcollected adult Phlebotomus sand flies under laboratory conditions. The fumigant activity was tested by exposing the sand flies to essential oil vapors at 10, 20, 50 and 100 µL/L air concentrations. Knock time 50 (KT50) values and the 95% confidence limits were calculated by using a probit analysis program. A decrease in the KT50 value is observed in S. cuneifolia and Z. clinopodioides essential oils in a concentration dependent manner. KT50 values at the highest concentration (100 µL/L air) are 9,3 and 11,6 min, respectively. After a 1 h exposure period, both test oils produced 100% mortality at all the concentrations tested. The results of the research suggest that essential oils from these two plants have a potential as control agents for sand flies. © by PSP.
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    Türkiye’nin Batısında, Leishmaniasis’in Endemik Olduğu Aydın Dağları ve Çevresinde Kum Sineği (Diptera: Psychodidae) Faunası, Mevsimsel Aktiviteleri ve Yüksekliğe Göre Dağılımları
    (NLM (Medline), 2022) Arserim S.K.; Mermer A.; Özbel Y.
    Objective: Human and canine leishmaniasis (CanL) are endemic in Turkey, and cutaneous leishmaniasis (CL) is a primary public health problem among vector-borne diseases, with 1500-2000 autochthonous cases per year. In this study, we aimed to perform a comprehensive entomological survey, including the detection of sand fly fauna, seasonal activity, and altitudinal distribution, together with the ecological features of Aydın Mountains and their surroundings that are endemic areas for CL caused by Leishmania tropica and CanL caused by L. infantum MON-1. Methods: The survey was carried out from June 2009 to July 2010. Ten localities with different altitudes were selected. Sand fly collection was done every 15 days in July and August when sand flies are most active and every 30 days between September and June (14 months, 16 samplings) using CDC light traps and sticky traps. During the collection, the temperature, humidity, and ecological and environmental features were recorded. Sand fly specimens were dissected and identified at species level using written keys. Results: A total of 6712 sand fly specimens (3268 females and 3444 males) were collected from 10 localities throughout all study periods. Species identification revealed that nine and three species belonging to Phlebotomus (P. major s.l. 30.38%, P. tobbi 22.93%, P. papatasi 5.88%, P. sergenti s.l. 4.51%, P. alexandri 4.26%, P. simici 3.50%, P. burneyi 0.63%, P. brevis 0.45%, and Transphlebotomus spp. 0.28%) and Sergentomyia (S. dentata 23.17%, S. minuta 2.43%, and S. antennata 1.58%) were found, respectively. The seasonal activities of these 12 sand fly species were determined, and P. major s.l. and P. tobbi, which were probable vectors of VL and CanL, were present in the study area. P. sergenti s.l., a probable vector of CL, was also found in the region and was active for seven months between April and October. P. sergenti s.l. was found in the 400-600 m altitude in July, while P. major and P. tobbi were found in high densities in the 200-400 m altitude in September. Conclusion: Our results showed that leishmaniasis vectors are present in different altitudes in the Aydın mountains and surroundings. The findings revealed the time intervals of parasite transmission in the area and the period of applying protective measures, such as insecticide application.Amaç: İnsan ve kanin leishmaniasis (CanL) açısından endemik bir ülke olan Türkiye’de, kutanöz leishmaniasis (KL) yılda 1500-2000 yerli olgu ile vektör kaynaklı hastalıklar arasında birincil halk sağlığı sorunudur. Bu çalışmada, Leishmania tropica’nın neden olduğu CL ve L. infantum MON-1’in neden olduğu CanL bakımından endemik bir alan olan Aydın Dağları ve çevresinde bulunan kum sineğinin faunası, mevsimsel aktiviteleri ve yüksekliğe göre dağılımları ile ekolojik özelliklerinin de belirlenmesini içeren kapsamlı bir entomolojik araştırma amaçlanmıştır. Yöntemler: Araştırma, Haziran 2009 ile Temmuz 2010 tarihleri arasında gerçekleştirilmiştir. Farklı yükseklik aralıklarına göre toplam 10 lokasyon seçilmiş, CDC ışık tuzakları ve yapışkan tuzaklar kullanılarak kum sineklerinin en aktif olduğu Temmuz ve Ağustos aylarında 15 günde bir, Eylül ve Haziran ayları arasında 30 günde bir olmak üzere kum sineği örnekleri toplanmıştır (toplam 14 ay, 16 örnekleme). Örnek toplama sırasında sıcaklık, nem, ekolojik ve çevresel özellikler kaydedilmiştir. Kum sineği örnekleri, teşhis anahtarları kullanılarak tür düzeyinde tanımlanmıştır. Bulgular: Tüm örnekleme periyotları boyunca, 10 lokasyondan toplam 6712 kum sineği örneği (3268 dişi ve 3444 erkek) toplanmıştır. Morfolojik tür teşhisinde Phlebotomus cinsine ait dokuz (P. major s.l. %30,38; P. tobbi %22,93; P. papatasi %5,88; P. sergenti s.l. %4,51; P. alexandri %4,26; P. simici %3,50; P. burneyi %0,63; P. brevis %0,45; Transphlebotomus spp. %0,28) ve Sergentomyia cinsine ait üç tür (S. dentata %23,17; S. minuta %2,43; S. antennata %1,58) bulunmuştur. On iki kum sineği türünün mevsimsel aktivitesi belirlenmiş, VL ve CanL’nin olası vektörleri olan P. major s.l. ve P. tobbi ile CL’nin olası vektörü olan P. sergenti s.l de çalışma alanında bulunmuş olup, Nisan ile Ekim ayları arasında yedi ay boyunca aktivite gösterdikleri saptanmıştır. P. sergenti s.l’nin Temmuz ayında 400-600 m’de, P. major ve P. tobbi’nin ise Eylül ayında 200-400 m yükseklik aralığında en fazla yoğunluğa sahip olduğu tespit edilmiştir. Sonuç: Sonuçlar, Aydın Dağları ve çevresinde leishmaniasis vektörlerinin farklı yüksekliklerde bulunduğunu göstermiştir. Elde edilen bulgular, çalışma alanında parazitin bulaşma dönemi ve insektisit uygulaması gibi koruyucu önlemlerin uygulama periyodunu ortaya koymuştur.