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    Cellular Biomarkers in Mytilus galloprovincialis L. (Mediterranean Mussels) from Izmir Bay (Turkey)
    (Springer, 2022) Katalay, Selma; Ayhan, Melike Merve; Guler, Cem; Aldag, Ceyda; Kilic, Tunay; Yavasoglu, N. Ulku Karabay
    In this study, cellular biomarkers and heavy metal concentration in the Mytilus galloprovincialis L. (Mediterranean mussels) collected from eight sites of Izmir Bay (Turkey) were determined to reveal water pollution for the first time in these stations. Results show that heavy metals (As, Cu, Hg, Zn, Cd, Sn, Pb) have been specified in mussels' tissues collected from all stations. According to GST, SOD, CAT activities, and TBARS contents, mussels in the outer bay have exposed more oxidative stress than the ones in the inner bay. Digestive gland tissues of them were showed more inhibition at AChE levels than gills. Also, abnormal nucleus rates and micronucleus frequencies (MN) were found to be higher in the inner bay than in the outer bay. This study showed that heavy metal pollution in different levels is an environmental issue on the Izmir Bay. Especially the coastal regions of the bay have been extremely affected by anthropogenic effects due to growing population.
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    * Concordance in molecular genetic analysis of tumour tissue, plasma, and exhaled breath condensate samples from lung cancer patients
    (Iop Publishing Ltd, 2020) Vardarli, Asli Tetik; Pelit, Levent; Aldag, Ceyda; Korba, Korcan; Celebi, Caglar; Dizdas, Tugberk Nail; Goksel, Tuncay
    Aim. Lung adenocarcinoma is characterized by poor prognosis and short survival rates. Therefore, tools to identify the tumoural molecular structure and guide effective diagnosis and therapy decisions are essential. Surgical biopsies are highly invasive and not conducive for patient follow-up. To better understand disease prognosis, novel non-invasive analytic methods are needed. the aim of the present study is to identify the genetic mutations in formalin-fixed paraffin-embedded (FFPE) tissue, plasma, and exhaled breath condensate (EBC) samples by next-generation sequencing and evaluate their utility in the diagnosis and follow-up of patients with lung adenocarcinoma. Method. FFPE, plasma, and EBC samples were collected from 12 lung adenocarcinoma patients before treatment. DNA was extracted from the specimens using an Invitrogen PureLink Genomic DNA Kit according to the manufacturer's instructions. Amplicon-based sequencing was performed using Ion AmpliSeq Colon and Lung Cancer Research Panel v2. Results. Genetic alterations were detected in all FFPE, plasma, and EBC specimens. the mutations in PIK3CA, MET, PTEN, SMAD4, and FGFR2 genes were highly correlated in six patients. Somatic and novel mutations detected in tissue and EBC samples were highly correlated in one additional patient. the EGFR p.L858R and KRAS p.G12C driver mutations were found in both the FFPE tissue specimens and the corresponding EBC samples of the lung adenocarcinoma patients. Conclusion. the driver mutations were detected in EBC samples from lung adenocarcinoma patients. the analysis of EBC samples represents a promising non-invasive method to detect mutations in lung cancer and guide diagnosis and follow-up.
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    How Safe Is the Use of Intrathecal Vancomycin?
    (Elsevier Science Inc, 2022) Demir, Abdulkadir; Camlar, Mahmut; Kuscu, Gokce Ceren; Gurel, Cevik; Oltulu, Fatih; Oren, Merve; Aldag, Ceyda
    BACKGROUND: Central nervous system infection after neurosurgical procedures is a severe complication with high morbidity rates and sometimes mortality. Our experimental study aimed to investigate the biochemical and histopathologic effects of vancomycin on neural tissues when applied to the cisterna magna. METHODS: Wistar albino rats were randomly divided into 4 groups: Control (Group 1) and different vancomycin dose groups (Groups 2, 3, and 4). In Group 1, 0.1 mL cerebrospinal fluid was drained from the cisterna magna and 0.1 mL 0.9% NaCI (normal saline) was administered into the subarachnoid space. In the study groups, 0.1 mL cerebrospinal fluid was drained from the cisterna magna and 0.1 mg/200g rat per day (Group 2), 0.2 mg/200g rat per day (Group 3), and 0.4 mg/200g rat per day (Group 4) vancomycin were administered into the subarachnoid space for 7 days. All rats were sacrificed on the eighth day. Serum super-oxide dismutase and catalase levels were measured. Histopathologic and immunohistochemical analyses were conducted. RESULTS: The findings showed that the administration of 0.2 and 0.4 mg/kg doses had significant differences in superoxide dismutase and catalase activity compared with the controls (P < 0.05). These vancomycin doses also induced the apoptotic process, and the enzyme activity results correlated with immunohistochemical results. CONCLUSIONS: Dose-related neurotoxicity of intrathecal vancomycin was shown at the cellular level. The importance of dose regulation of intrathecal vancomycin has come into view. To our knowledge, this is the first study in the literature that has investigated the neurotoxic effects of vancomycin.