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Öğe Active-Matrix Metalloproteinase-8, Myeloperoxidase in Relation With Periodontics, Preterm Birth(Wiley, 2024) Alan, Murat; Sorsa, Timo; Meric Kantar, Pinar; Raisanen, Ismo T.; Gurlek, Onder; Kanmaz, Burcu; Buduneli, NurcanObjective: To investigate serum, placental levels of active-matrix metalloproteinase-8 (aMMP-8), myeloperoxidase (MPO) in preterm-birth with/without pre-eclampsia and term counterparts in relation with clinical periodontal parameters. Methods: Clinical periodontal measurements were recorded. Serum and placenta samples were collected during 173 full-term (FT), pre-term (PT) or pre-term complicated by pre-eclampsia (PTPE) deliveries. aMMP-8 levels were measured by IFMA. MPO levels in the serum and placenta samples were determined by ELISA. Data were tested using non-parametric tests. Results: PTBE group exhibited higher full-mouth probing depth and clinical attachment loss values than the other two groups (p < 0.05). Percentages of sites with plaque and bleeding on probing were lower in the PTBE group than in the other groups (p < 0.05). Serum aMMP-8 and MPO concentrations were higher in PTPE group than in the other groups (p < 0.05). Placenta aMMP-8 level was higher in the control group than in the PTPE group (p < 0.05). There was no significant difference between the groups in the placenta MPO levels (p > 0.05). Conclusions: Within the limits of this cross-sectional study, it may be suggested that serum aMMP-8 and MPO concentrations together with placenta aMMP-8 levels may be associated with and reflect adverse pregnancy outcomes. Clinical periodontal findings did not reveal significant associations with these proteolytic and oxidative biomarkers.Öğe Asprosin: a novel peptide hormone related to insulin resistance in women with polycystic ovary syndrome(Taylor & Francis Ltd, 2019) Alan, Murat; Gurlek, Beril; Yilmaz, Alpay; Aksit, Murat; Aslanipour, Behnaz; Gulhan, Ibrahim; Mehmet, Calan; Taner, Cuneyt EftalAsprosin associated with insulin resistance is a newly discovered peptide hormone. The peptide promotes hepatic glucose production. Polycystic ovary syndrome (PCOS) is a metabolic disorder. Insulin resistance plays a vital role in the pathogenesis of the disease. The aim of this study was to discover the association between insulin resistance and asprosin in women with PCOS. We recruited 78 subjects with PCOS and 78 age-matched and body mass index (BMI)-matched controls into this cross-sectional study. Circulating asprosin levels were validated using ELISA method. We also determined metabolic and hormonal parameters of the involved subjects. We found that circulating asprosin levels were elevated in women with PCOS with respect to controls. Asprosin levels showed a positive correlation with insulin resistance, BMI, and free androgen index (FAI). Moreover, subjects with the highest tertile of asprosin levels represented increased odds of having PCOS as compared to those subjects with the lowest tertile asprosin levels. Increased asprosin levels resulted to high possibility of having PCOS risk associated with insulin resistance.Öğe Association of decreased C1q/tumor necrosis factor- related protein-5 levels with metabolic and hormonal disturbance in polycystic ovary syndrome(Galenos Yayincilik, 2019) Calan, Mehmet; Alan, Murat; Alarslan, Pinar; Kocabas, Gokcen Unal; Bozkaya, Giray; Acara, Ahmet Cagdas; Aslanipour, Behnaz; Fenercioglu, Ozge; Isil, Ahmet Murat; Guler, AsliObjective: C1q/tumor necrosis factor-related protein-5 (CTRP5) is a novel peptide hormone involved in the metabolism of energy regulation. Polycystic ovary syndrome (PCOS), which is a reproductive and metabolic disorder, is associated with insulin resistance. The aim of the current study was to compare circulating levels of CTRP5 in women with and without PCOS and to investigate possible associations between CTRP5 and metabolic-hormonal parameters. Material and Methods: The present cross-sectional study contained 80 women with PCOS and 80 age and body mass index-matched women without PCOS. Circulating levels of CTRP5 were calculated using an enzyme-linked immunosorbent assay. We also measured hormonal and metabolic parameters. Results: Patients with PCOS had lower levels of circulating CTRP5 compared with women without PCOS (6.90 +/- 2.64 vs 11.73 +/- 3.66 ng/mL, p<0.001). CTRP5 was negatively correlated with insulin resistance, free-androgen index, and body mass index in both the PCOS and control groups. Moreover, patients with PCOS who had insulin resistance showed lower circulating CTRP5 levels compared with those without insulin resistance. In both the control and PCOS groups, overweight subjects had lower circulating levels of CTRP5 compared with participants of normal weight. Logistic regression analyses indicated that subjects in the lowest tertile for CTRP5 level had higher risk for PCOS compared with those in the highest tertile of CTRP5. Conclusion: Decreased circulating levels of CTRP5 were associated with higher risk of PCOS, as well as having metabolic disturbance among women with PCOS.Öğe Association of decreased C1q/tumor necrosis factor-related protein-5 levels with metabolic and hormonal disturbance in polycystic ovary syndrome(2019) Çalan, Mehmet; Alarslan, Pınar; Kocabaş, Gökçen Ünal; Alan, Murat; Bozkaya, Özlem Giray; Fenercioğlu, Özge; Aslanipour, BehnazObjective: C1q/tumor necrosis factor-related protein-5 (CTRP5) is a novel peptide hormone involved in the metabolism of energy regulation. Polycystic ovary syndrome (PCOS), which is a reproductive and metabolic disorder, is associated with insulin resistance. the aim of the current study was to compare circulating levels of CTRP5 in women with and without PCOS and to investigate possible associations between CTRP5 and metabolic-hormonal parameters.Material and Methods: the present cross-sectional study contained 80 women with PCOS and 80 age and body mass index-matched women without PCOS. Circulating levels of CTRP5 were calculated using an enzyme-linked immunosorbent assay. We also measured hormonal and metabolic parameters.Results: Patients with PCOS had lower levels of circulating CTRP5 compared with women without PCOS (6.90±2.64 vs 11.73±3.66 ng/mL, p<0.001). CTRP5 was negatively correlated with insulin resistance, free-androgen index, and body mass index in both the PCOS and control groups. Moreover, patients with PCOS who had insulin resistance showed lower circulating CTRP5 levels compared with those without insulin resistance. in both the control and PCOS groups, overweight subjects had lower circulating levels of CTRP5 compared with participants of normal weight. Logistic regression analyses indicated that subjects in the lowest tertile for CTRP5 level had higher risk for PCOS compared with those in the highest tertile of CTRP5.Conclusion: Decreased circulating levels of CTRP5 were associated with higher risk of PCOS, as well as having metabolic disturbance among women with PCOS. (J Turk Ger Gynecol Assoc 2019; 20: 89-96)Öğe Decreased levels of liver-expressed antimicrobial peptide-2 and ghrelin are related to insulin resistance in women with polycystic ovary syndrome(Taylor & Francis Ltd, 2020) Aslanipour, Behnaz; Alan, Murat; Demir, IsmailLiver-expressed antimicrobial peptide 2 (LEAP-2) is a newly identified peptide hormone involved in glucose metabolism. It acts as a noncompetitive antagonist of ghrelin hormone's receptor. Polycystic ovary syndrome (PCOS) is a common metabolic and reproductive disease associated with insulin resistance. We aimed to compare circulating LEAP-2 levels in subjects with PCOS and controls. We also focused to determine whether there was a relationship between LEAP-2 and metabolic parameters in women with PCOS. We enrolled 64 subjects with PCOS and 64 age and body mass index (BMI)-matched controls into the current cross-sectional study. Circulating LEAP-2 and ghrelin levels were measured via ELISA method. Metabolic and hormonal parameters of the involved subjects were analyzed. We found that circulating LEAP-2 and ghrelin levels were decreased in women with PCOS as compared with controls. LEAP-2 showed a positively independent association with ghrelin while LEAP-2 exhibited an inverse association with insulin resistance, BMI, and free-androgen index (FAI). Additionally, subjects having the lowest tertile of LEAP-2 were in positive link of developing PCOS risk with respect to those subjects having the highest tertile of LEAP-2 levels. Decreased LEAP-2 levels were associated with a high possibility of having PCOS risk associated with insulin resistance.Öğe Decreased levels of liver-expressed antimicrobial peptide-2 and ghrelin are related to insulin resistance in women with polycystic ovary syndrome(Taylor & Francis Ltd, 2019) Aslanipour, Behnaz; Alan, Murat; Demir, IsmailLiver-expressed antimicrobial peptide 2 (LEAP-2) is a newly identified peptide hormone involved in glucose metabolism. It acts as a noncompetitive antagonist of ghrelin hormone's receptor. Polycystic ovary syndrome (PCOS) is a common metabolic and reproductive disease associated with insulin resistance. We aimed to compare circulating LEAP-2 levels in subjects with PCOS and controls. We also focused to determine whether there was a relationship between LEAP-2 and metabolic parameters in women with PCOS. We enrolled 64 subjects with PCOS and 64 age and body mass index (BMI)-matched controls into the current cross-sectional study. Circulating LEAP-2 and ghrelin levels were measured via ELISA method. Metabolic and hormonal parameters of the involved subjects were analyzed. We found that circulating LEAP-2 and ghrelin levels were decreased in women with PCOS as compared with controls. LEAP-2 showed a positively independent association with ghrelin while LEAP-2 exhibited an inverse association with insulin resistance, BMI, and free-androgen index (FAI). Additionally, subjects having the lowest tertile of LEAP-2 were in positive link of developing PCOS risk with respect to those subjects having the highest tertile of LEAP-2 levels. Decreased LEAP-2 levels were associated with a high possibility of having PCOS risk associated with insulin resistance.Öğe Determination and Comparison of in Vitro Radical Scavenging Activity of Both Garlic Oil and Aqueous Garlic Extracts and Their in Vivo Antioxidant Effect on Schistosomiasis Disease in Mice(2018) Sadrefozalayi, Somayyeh; Aslanipour, Behnaz; Alan, Murat; Çalan, MehmetGarlic has long been utilizing as a folk remedy for various diseases. in this study, the effect of aqueous garlic extract (AGE) (125 mg kg-1), garlic oil extract (GOE) (125 mg kg-1), and the combination of both extracts (AGE+GOE) (62.5 from each) was studied over a parasitic disease namely schistosomiasis. Determination of some radical scavenging activities including Ferric Reducing Ability Power, Hydrogen peroxide, DPPH and nitric oxide radical scavenging activities were studied. the antioxidant properties of the mentioned extracts on both normal and Schistosoma mansoni-infected mice was also studied. the liver tissues were analyzed for the level of Malondialdehyde (MDA) and glutathione (GSH) known as biomarkers for oxidative and reducing status, respectively. the results showed that almost all tested extracts showed high effect of radical scavenging activity. Furthermore, remarkable decrease in worms and tissue eggs were analyzed in all tested extracts as AGE+ GOE (1015 ± 1.41) represented higher level of prohibition in total number of worms and eggs compared to infected untreated mice (1502 ± 2.13). the garlic extracts were potent antioxidants with slight differences.Öğe Effects of regenerative cutting on the height growth of Turkey oak (Quercus cerris L.) saplings(2020) Kavgacı, Ali; Örtel, Erdal; Alan, Murat; Çobanoğlu, AbdurrahmanTurkey oak (Quercus cerris) is distributed at the northern, western and southern Anatolia in Turkey. Although these forests are managed by shelterwood systems, degraded forests are subjected to artificial regeneration. In this context, the mature Turkey oak stands in Sipahiler Forests sub-district affiliated Sütçüler Forest District Directorate in Isparta, which did not have natural regeneration conditions, were artificially regenerated by acorn point sowing under the stand shelter. Although the regeneration was successful, the seedlings did not realize sufficient growth. In this context, regenerative cuttings (1- cutting all saplings in the plots from just above the ground level, 2- Cutting saplings from 10 cm height above the ground level, 3- Leaving only one individual at the each sowing plot (excluding the lateral stems) and pruning, 4-Leaving only one individual at the each sowing plot (excluding the lateral stems) without pruning and 5- Control without any cutting and pruning) were applied to the saplings and the 4 years’ height growths of saplings were measured. The treatments were realized just before the vegetation period of 2014 and first measurements were done after the cuttings. To determine the height increment, second measurements were carried out after the vegetation period of 2017. As a result of these measurements, the height growth increment for each treatment was realized as follow: 75.25 cm for the saplings cut from ground level, 61.98 cm for the saplings cut from 10 cm height from ground level, 33.62 cm for the saplings of thinned and pruning, 32.80 cm for the saplings of thinned without pruning and 32.80 for the saplings of control. In final, the height growth of regenerative cutting saplings reached to those which were not subjected to cuttings. These results showed that regenerative cutting is promising to foster the growth of Turkey oak saplings. But for a better understanding, longer observation is needed to see the growth differences between the saplings subjected to regenerative cuttings and others, since the height of regenerative cutting saplings is not higher than the others yet. Keywords: artificial regeneration, degradation, sapling, sproutingÖğe Effects of regenerative cutting on the height growth of Turkey oak (Quercus cerris L.) saplings(2020) Kavgacı, Ali; Örtel, Erdal; Alan, Murat; Çobanoğlu, AbdurrahmanTurkey oak (Quercus cerris) is distributed at the northern, western and southern Anatolia in Turkey. Although these forests are managed by shelterwood systems, degraded forests are subjected to artificial regeneration. in this context, the mature Turkey oak stands in Sipahiler Forests sub-district affiliated Sütçüler Forest District Directorate in Isparta, which did not have natural regeneration conditions, were artificially regenerated by acorn point sowing under the stand shelter. Although the regeneration was successful, the seedlings did not realize sufficient growth. in this context, regenerative cuttings (1- cutting all saplings in the plots from just above the ground level, 2- Cutting saplings from 10 cm height above the ground level, 3- Leaving only one individual at the each sowing plot (excluding the lateral stems) and pruning, 4-Leaving only one individual at the each sowing plot (excluding the lateral stems) without pruning and 5- Control without any cutting and pruning) were applied to the saplings and the 4 years’ height growths of saplings were measured. the treatments were realized just before the vegetation period of 2014 and first measurements were done after the cuttings. To determine the height increment, second measurements were carried out after the vegetation period of 2017. As a result of these measurements, the height growth increment for each treatment was realized as follow: 75.25 cm for the saplings cut from ground level, 61.98 cm for the saplings cut from 10 cm height from ground level, 33.62 cm for the saplings of thinned and pruning, 32.80 cm for the saplings of thinned without pruning and 32.80 for the saplings of control. in final, the height growth of regenerative cutting saplings reached to those which were not subjected to cuttings. These results showed that regenerative cutting is promising to foster the growth of Turkey oak saplings. But for a better understanding, longer observation is needed to see the growth differences between the saplings subjected to regenerative cuttings and others, since the height of regenerative cutting saplings is not higher than the others yet. Keywords: artificial regeneration, degradation, sapling, sproutingÖğe Placental TLR recognition of salivary and subgingival microbiota is associated with pregnancy complications(Bmc, 2024) Pax, Kazune; Buduneli, Nurcan; Alan, Murat; Meric, Pinar; Gurlek, Onder; Dabdoub, Shareef M.; Kumar, Purnima S.Background Pre-term birth, the leading cause of neonatal mortality, has been associated with maternal periodontal disease and the presence of oral pathogens in the placenta. However, the mechanisms that underpin this link are not known. This investigation aimed to identify the origins of placental microbiota and to interrogate the association between parturition complications and immune recognition of placental microbial motifs. 61FbVQciH1C2BbwKYgvy28 Video AbstractBackground Pre-term birth, the leading cause of neonatal mortality, has been associated with maternal periodontal disease and the presence of oral pathogens in the placenta. However, the mechanisms that underpin this link are not known. This investigation aimed to identify the origins of placental microbiota and to interrogate the association between parturition complications and immune recognition of placental microbial motifs. 61FbVQciH1C2BbwKYgvy28 Video AbstractMethods Saliva, plaque, serum, and placenta were collected during 130 full-term (FT), pre-term (PT), or pre-term complicated by pre-eclampsia (PTPE) deliveries and subjected to whole-genome shotgun sequencing. Real-time quantitative PCR was used to measure toll-like receptors (TLR) 1-10 expression in placental samples. Source tracking was employed to trace the origins of the placental microbiota.Results We discovered 10,007 functionally annotated genes representing 420 taxa in the placenta that could not be attributed to contamination. Placental microbial composition was the biggest discriminator of pregnancy complications, outweighing hypertension, BMI, smoking, and maternal age. A machine-learning algorithm trained on this microbial dataset predicted PTPE and PT with error rates of 4.05% and 8.6% (taxonomy) and 6.21% and 7.38% (function). Logistic regression revealed 32% higher odds of parturition complication (95% CI 2.8%, 81%) for every IQR increase in the Shannon diversity index after adjusting for maternal smoking status, maternal age, and gravida. We also discovered distinct expression patterns of TLRs that detect RNA- and DNA-containing antigens in the three groups, with significant upregulation of TLR9, and concomitant downregulation of TLR7 in PTPE and PT groups, and dense correlation networks between microbial genes and these TLRs. 70-82% of placental microbiota were traced to serum and thence to the salivary and subgingival microbiomes. The oral and serum microbiomes of PTPE and PT groups displayed significant enrichment of genes encoding iron transport, exosome, adhesion, quorum sensing, lipopolysaccharide, biofilm, and steroid degradation.Conclusions Within the limits of cross-sectional analysis, we find evidence to suggest that oral bacteria might translocate to the placenta via serum and trigger immune signaling pathways capable of inducing placental vascular pathology. This might explain, in part, the higher incidence of obstetric syndromes in women with periodontal disease.
