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    Assessing genetic diversity of some potato (Solanum tuberosum L.) genotypes grown in turkey using the AFLP marker technique
    (2010) Akkale C.; Yildirim Z.; Yildirim M.B.; Kaya C.; Öztürk G.; Tanyolaç B.
    In the present study, the level of polymorphism and the genetic relationships among 26 potato genotypes were studied by means of molecular markers using the amplified fragment length polymorphism (AFLP) technique. DNA was extracted from fresh leaves of the seedlings. Selective amplification products revealed a total of 191 polymorphic bands ranging from 8 to 45 for each combination. Scoring results were used to generate a tree in JMP software. The 26 samples formed six clades with varying number of members between one and eleven. Genetic distances among genotypes were calculated according to Jaccard's formula, in Phylip 3.0 software. According to the results of genetic distance, dendrogram showed that genotypes 6/7-4 and 6/7-2 were the closest genotypes with a genetic distance of 0.13. On the other hand, genotypes Posof-10 and Marabel were the most distinct from each other with a genetic distance value of 0.55. The AFLP marker results showed a great consistency along with their pedigrees indicating the AFLP technique as a useful tool in the calculation of genetic distance of the potato genotypes.
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    Screening of resistance genes to fusarium root rot and fusarium wilt diseases in F3 family lines of tomato (Lycopersicon esculentum) using RAPD and CAPs markers
    (2010) Tanyolaç B.; Akkale C.
    Fusarium diseases constitute most of the loss in tomato production worldwide, because it spreads on all geographic fields that it is so hard to find a place without fusarium infestation. Thus, the best way to produce tomato is developing resistant cultivars against Fusarium species. In cultivar developing, molecular marker assisted techniques replaced traditional breeding techniques which are high cost and time consuming for breeders. In this study RAPD and CAPS markers were used to screen tomato (Lycopersicon esculentum) lines against resistance genes Frl and I-2, respectively. Results showed that out of 115 plants, 42 were homozygous resistant, 38 were heterozygous resistant and 35 were homozygous susceptible. Under the light of this information, the forthcoming cultivar development studies will be carried out. © 2010 Academic Journals.