Yazar "Ag Seleci, Didem" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil
    (Scientific Technical Research Council Turkey-Tubitak, 2015) Ag Seleci, Didem; Gumus, Zinar Pinar; Yavuz, Murat; Seleci, Muharrem; Bongartz, Rebecca; Stahl, Frank; Coskunol, Hakan; Timur, Suna; Scheper, Thomas
    The objectives of this study were to investigate the potential biological activities of black cumin seed oil (BCSO) and wheat germ oil (WGO) on different cell lines. Initially, commercially available BCSO and WGO obtained by supercritical carbon dioxide extraction were analyzed in terms of tocopherol, aliphatic alcohols, and thymoquinone content via HPLC and GC analysis. Cell free antioxidant activities and total phenolic content of both oils were detected by DPPH assay and Folin-Ciocalteu method, respectively. As well as the DPPH assay, the protective effect against reactive oxygen species (ROS) was determined by microscopic observation of ROS generation in NIH-3T3 cells with or without oil samples by using an oxidation-sensitive fluorescent dye, H2DCFDA. Cytotoxicity was assessed using an MTT assay. In the case of BCSO, after exposing cells to 0.025-1.0 mg/mL and 1.0-100 mu g/mL concentrations for 24 h, the IC50 values of BCSO were 0.58, 0.51, 0.47, and 0.36 mg/mL for NIH-3T3, A549, U87, and HeLa cells, respectively. On the other hand, concentrations of WGO lower than 0.1 mg/mL did not cause a decrease in cell viability for all cell lines. Apoptotic and necrotic rates of these cell lines were investigated via flow cytometry. BCSO also exhibited proliferative efficacy for NIH-3T3 cells.
  • Küçük Resim Yok
    Öğe
    Transferrin-Decorated Niosomes with Integrated InP/ZnS Quantum Dots and Magnetic Iron Oxide Nanoparticles: Dual Targeting and Imaging of Glioma
    (Mdpi, 2021) Ag Seleci, Didem; Maurer, Viktor; Barlas, Firat Baris; Porsiel, Julian Cedric; Temel, Bilal; Ceylan, Elcin; Timur, Suna
    The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEGNIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma.