Yazar "çiçek C." seçeneğine göre listele

Listeleniyor 1 - 1 / 1
  • Küçük Resim Yok
    Öğe
    Detection of adenovirus frequency in cases with gastroenteritis symptoms by shell-vial cell culture [Gastroenterit semptomlari olan olgularda adenovirüs sikliğinin shell-vial hücre kültürü yöntemi ile saptanmasi]
    (Refik Saydam National Public Health Agency (RSNPHA), 2019) Aksoy-Gökmen A.; çiçek C.; Kalfaoğlu H.; Saz E.U.
    Objective: Gastroenteritis which is caused by viruses are common all over the world. It is a very important health problem and a leading cause of mortality for children in developed and developing countries. Among the viral pathogens, rotavirus (25-65%) and enteric adenovirus (5-15%) are the most common. In this study, stool specimens of patients sent to Ege University Medical Faculty Medical Microbiology Department Virology Laboratory between January 2010 and March 2014 for prediagnosis of gastroenteritis were examined. It is aimed to determine the frequency of adenovirus in stool specimens by shell-vial cell culture technique and to investigate its distribution according to age and season. Methods: A retrospective study of 264 patients with acute gastroenteritis were included in the study. The shell-vial cell culture method and HEp-2 cell line (German Collection of Microorganisms and Cell Cultures, DSMZ, Germany) were used for adenovirus isolation. All of the operations were done in second level biosecurity cabinets. A cell culture tube was prepared for each patient. In the detection of adenovirus, monoclonal antibody (Light Diagnostic, Millipore, USA) labeled with effect specific fluorescein isothiocyanate was used at the end of the two-day incubation period. The fluorescence microscope (Olympus BX50, Japan) was evaluated at 20X magnification and 40X magnification. Specimens that gave at least two or more of the cells a typical apple green fluorescence were considered positive. Results: Of the 264 patients included in the study with acute gastroenteritis pre-diagnosis, 190 (70%) were children (72%) and 74 (%28) were adults. 13 (4,9%) of the 264 stool samples sent to the virology laboratory had adenovirüs positivity with the shell-vial cell culture technique. All positive samples were under 18 years of age. Of the cases, 111 were female (42%) and 153 were male (58%). There was no significant difference between genders in terms of adenovirus positive rate (p > 0.05). In terms of seasonal frequency, adenovirüs was found to be the lowest in summer (0.4%) and the highest in winter (by shell vial cell culture in stool. Conclusion: Although we think that enteric adenoviral agent should be investigated routinely in the gastroenteritis cases in our region, the data obtained from our work done with cell culture, which is the gold standart method, contribute to the epidemiological data. © 2019, Refik Saydam National Public Health Agency (RSNPHA).