DSpace Arşivi :: by Yazar "Çiçek C." değerine göre listeleniyor

Yazar "Çiçek C." seçeneğine göre listele

Listeleniyor 1 - 17 / 17

Comparison of Clinical Presentations and Disease Severity of Children Hospitalized with Influenza A and B
(AVES, 2024) Eşki A.; Öztürk G.K.; Çiçek C.; Gülen F.; Demir E.
Objective: This study compared the clinical presentations and disease severity between influenza A and B (FLUA and FLUB). Materials and Methods: The study included children hospitalized with virologically confirmed influenza between 2010 and 2020. The severity of the disease was evaluated based on admission to the pediatric intensive care unit (PICU), mechanical ventilation requirement, length of hospital stay, length of stay in the PICU, and death. Influenza viruses were compared within predefined age groups (0-2, 3-9, and 10-18 years) and in all age groups. Results: Of 343 patients, FLUA and FLUB were detected in 75.8% and 24.2% of children, respec-tively. FLUB was associated with a higher incidence of headache and abdominal pain (P <.001 and P =.01). Children with FLUB were prescribed antibiotics and antivirals 0.56 and 0.58-fold fewer than those with FLUA. Headache and abdominal pain rates were higher in patients between 3 and 9 years with FLUB. Children between 0 and 2 years with FLUA were more fre-quently admitted to the PICU than those with FLUB (23.6% vs. 4.0%; P <.004). Eight patients with FLUA died, while only 1 with FLUB died (P =.69). Conclusion: The clinical presentation of FLUA and FLUB appeared similar, except for headache and abdominal pain, which were more prevalent in older patients with FLUB. Our study revealed that children between 0 and 2 years with FLUA were at a significantly higher risk for admission to the PICU. As a result, greater attention and awareness should be paid to children under 2 years old with FLUA. © 2024, AVES. All rights reserved.
Comparison of interferon-gamma whole blood assay with tuberculin skin test for the diagnosis of tuberculosis infection in tuberculosis contacts [Temasl?larda tüberküloz enfeksiyonunun tan?s? için interferon-gama tam kan testi ile tüberkülin deri testinin karş?laşt?r?lmas?]
(2007) Öztürk N.; Sürücüoglu S.; Özkütük N.; Gazi H.; Akçali S.; Köroglu G.; Çiçek C.
Tuberculin skin test which is used for the detection of latent tuberculosis (TB), has many disadvantages such as false positivities due to cross reactions between environmental mycobacteria and BCG strain, false negativities due to immunosuppression and malpractice, and also difficulties in application and evaluation. Recently a new diagnostic test which measures the production of interferon (IFN)-gamma in whole blood upon stimulation with specific ESAT-6 and CFP-10 antigens of Mycobacterium tuberculosis has been introduced. Since most of the mycobacteria other than tuberculosis and BCG strain do not contain these antigens, the detection of IFN-gamma levels indicates the specific T-cell response against M.tuberculosis. The aim of the study was to compare the tuberculin skin test and whole blood IFN-gamma assay (QuantiFERON®-TB Gold, Cellestis Ltd, Carnegie, Victoria, Australia) for the identification of latent TB infection in the contacts with active TB patients. The tests results were evaluated by using Kappa (K) analysis, and K coefficients of <0.4, 0.4-0.75 and >0.75 were accepted as poor, moderate and excellent agreements, respectively. A total of 233 subjects from three risk groups were included to the study. Group 1 included the household members (n=133) who had contact with smear positive index cases, Group 2 included the subjects from community (n=46) who had contact with smear positive index cases, and Group 3 included health care workers (n=74) who had contact with TB patients or their specimens. The positivity rates of tuberculin skin test and IFN-gamma assay in the cases were found as 37% and 42%, respectively. There were no significant differences among the three patient groups with regard to the results of the tuberculin skin test (p>0.05). However, the positive result of the IFN-gamma assay in Group 1 was found statistically higher than the other groups (51.3%, p=0.013). A poor agreement between the two tests was detected in the results taken from 233 subjects (65.7%, K=0.28), while agreement was moderate in unvaccinated group (72.7%, K=0.44). Evaluation of agreement rates of the tests according to the risk groups yielded 64.6% (K=0.3) for Group 1, 71.7% (K=0.32) for Group 2, and 63.5% (K=0.21) for Group 3, which all coefficients showed poor agreement. Although IFN-gamma blood assay has many advantages such as objective and quantitative results, no interference with vaccination due to the use of specific antigens and being practical, the high cost and the need for well-equipped laboratory are its disadvantages. As a result it was concluded that, IFN-gamma blood assay has limited value for the detection of latent TB infection in our country, since the prevalence of TB infection and BCG vaccination rates are high in Turkey.
Detection of human metapneumovirus prevalence in pediatric patients with lower respiratory tract infections [Alt solunum yolu enfeksiyonu olan pediatrik hastalarda insan metapnömovlrus prevalansinin saptanmasi]
(2012) Gökmen A.A.; Çiçek C.; Saz E.U.; Özananar Y.; Duyu M.
Human metapneumovirus (hMPV) which is classified in Paramyxoviridae family has been identified in 2001 as the etiological agent of lower respiratory tract infection (LRTI) especially in children. Previous studies indicated that hMPV prevalence in LRTI is between 2-25%, being resposible for 10% of childhood LRTIs and its isolation rate is approximately 6% in hospitalized patients under age three years. The aim of this study was to investigate the hMPV prevalence in children with LRTI in our region. A total of 100 patients (41 female, 59 male) ages between 0-10 years old (median age: 4.8) and who were admitted to Pediatric Clinics of Ege University Medical Faculty Hospital with the diagnosis of LRTI between )a-nuary-December 2009 were included in the study. Nasopharyngeal swab samples were taken from those patients during the first three days of their symptoms. The presence of hMPV in the samples were investigated by rapid (shell vial) cell culture method using HEp-2 cell line and by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). The methods were performed to the clinical samples simultaneously. In both methods, a standard strain of hMPV provided by Erasmus University was used as positive control and QCMD-2009 hMPV panel was used as external quality control. In our study, 11 and 2 samples were found positive with cell culture and rRT-PCR methods, respectively. Two of rRT-PCR positive samples were also positive in cell culture, while the other nine were positive by only cell culture method. Both of the methods were performed twice due to inconsistent results, however, the same results were obtained in both runs. Studies with QCMD-2009 panel yielded compatible results for five samples, however a positive standard sample (hMPV A subtype, Ct value: 37.31) was found as negative by rRT-PCR test used in this study (RealAccurateTM, Pathofinder, The Netherlands). Our data showed that the prevalence of hMPV detected by rapid cell culture method was 11 % in pediatric patients with LRTIs, the age range of hMPV positive cases was 6 months to 7 years old (median age: 20 months), the majority of the admissions was in winter season and the main clinical picture was bronchiolitis. In addition, rRT-PCR assay used in this study was thought to be insufficient to detect the viral RNA in the event of low levels of hMPV A subtypes. Thereby the cell culture method should be used in addition to the new developing molecular methods for the detection of hMPV until standardization is achieved.
Development of a database for tracking HIV positive/AIDS patients [HIV pozi·ti·f/aids hastalarinin tani ve i·zlemi· i·çi·n geli·şti·ri·len veri· tabani ortami]
(2007) Altuglu I.; Çavuşoglu C.; Çiçek C.; Tünger Ö.
The collection of reliable data is the first step to assess the status of HIV/AIDS in a community. HIV recording systems are necessary for organizing and analyzing the patients' data. The aim of the study was to develop a database to be used to track HIV positive/AIDS patients. The database includes general demographic fields as well as specific fields such as health history, laboratory and other clinical history, current and past drug regimens (both antiretroviral and non-antiretroviral drugs). It is also possible to organize and maintain a patient database according to specific diseases, laboratory tests and/or medication treatments.
Evaluation of viral etiology in central nervous system infections from a university hospital point of view in izmir based on seven years data [Santral Sinir Sistemi Enfeksiyonlarinda Viral Etiyolojinin izmir'de Bir Üniversite Hastanesinin Yedi Yillik Verileri Üzerinden Degerlendirilmesi]
(Ankara Microbiology Society, 2017) Zeytinoglu A.; Erensoy S.; Sertoz R.; Altuglu I.; Çiçek C.; Kayin M.; Şirin H.; Taner S.
The serious diseases of the central nervous system (CNS); encephalitis and meningitis, have high mortality and morbidity rate especially not diagnosed and treated in time. Nucleic acid testing (NAT) is the tool of choice for viral diagnosis in CNS infections. In this study, viral etiological agents found in cerebrospinal fluid (CSF) samples sent to our university hospital virology laboratory for laboratory diagnosis of CNS infections were retrospectively evaluated and results were compared with other reports from our country. Viral etiological agents found in cerebrospinal fluid (CSF) samples sent to Ege University Faculty of Medicine Department of Medical Microbiology Virology Laboratories for laboratory diagnosis of CNS infection between 01.01.2009-31.12.2015 were evaluated retrospectively. A total of 3778 CSF tests were performed for cell culture of enterovirus (EV) in 487 samples and 3291 tests for nucleic acid testing (NAT) by real time polymerase chain reaction (PCR) in herpes simplex virus 1 (HSV1), herpes simplex virus 2 (HSV2), varicella zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpes virus 6 (HHV6) and EV. VZV and EV NAPs were performed during the last one and five years period, respectively. NAT positive results for HSV1, HSV2, CMV, EBV, VZV, HHV6 and EV were 1.80% (24/1333), 0.08% (1/1333), 3.28% (19/580), 4.35% (22/506), 0.46% (1/216), 1.05% (5/478) and 3.37% (6/178), respectively. EV was isolated in 30 (6.20%) of 487 CSF samples by viral culture. Positive samples were mainly from pediatric, neurology and infectious diseases clinics as expected. The number of higher positive results were found in samples sentin December (35.3%), July (12.9%) and November (10.6%). Overall 80% of positive samples belonged to patients over 18 years old. When the results of other studies reported from Turkey are examined, although the positivity rates are generally similar, it is seen that the rates specific to certain factors are higher in selected smaller patient groups like HSV1 and EV. Rapid nucleic acid tests like multiplex PCR and microarray will provide more practical and effective laboratory diagnosis approach in CNS infections, since many more microorganisms may be causative agents.
The first BVR light curves and period analysis of the eclipsing binary V821 Cas
(2007) Degirmenci O.L.; Bozkurt Z.; Yakut K.; Demircan O.; Erdem A.; Çiçek C.; Bulut I.; Soydugan F.; Soydugan E.; Esenoglu H.
The first BVR light curves of the eclipsing binary V821 Cas are used to derive the physical parameters of the system. The light curves were obtained during 2000 and 2001 observational seasons at Ege University Observatory (EUO). Period variation of the system was also investigated. For the first time apsidal motion elements of the system were obtained from the O-C curve analyses. Preliminary absolute parameters of the components were computed taking into account the results of light curves analysis. Observational and theoretical internal structure constants were estimated to be log over(k, ¯)2,obs = - 2.66 and log over(k, ¯)2,theo = - 2.42, respectively. © 2006 Elsevier B.V. All rights reserved.
Identification of enteroviruses from central nervous system infections by RT-PCR and cell culture methods [Santral sinir sistemi enfeksiyonu etkeni enteroviruslarin RT-PCR ve hücre kültür yöntemleri ile saptanmasi]
(2011) Kiliç I.; Altuglu I.; Çiçek C.; Pullukçu H.; Bayram N.; Şirin H.; Erensoy S.
Viruses are the major causes of aseptic meningitis and encephalitis. Enteroviruses account for more than 80% of the aseptic meningitis cases for which an etiologic agent is identified. The aims of the present study were to identify agents of enteroviral meningitis by viral culture and reverse transcriptase polymerase chain reaction (RT-PCR) methods, to evaluate the appropriateness of a commercial RTPCR kit for its use in routine laboratory, and to obtain epidemiological data about enteroviral meningitis. Sixty six cerebrospinal fluid (CSF) samples from patients with suspected viral central nervous system (CNS) infection by clinical and CSF biochemical findings, sent to Ege University Faculty of Medicine, Department of Medical Microbiology were included in the study. The CSF samples were all negative for tested bacteria, mycobacteria, fungi, herpes simplex virus and cytomegalovirus. Thirty-four (51.5%) of the samples were from female and 32 (48.5%) were from male patients. Twenty-three (34.8%) patients were children (5 months-18 years) and 43 (65.2%) were adults (19-86 years). Shell vial rapid cell culture method by using Vero, HEp-2 and RD cell lines was performed for virus isolation and the results were evaluated on 48 th hours after staining the cells with fluorescein labeled polyclonal antibodies (Pan-Enterovirus Blend, Light Diagnostics, USA). Enteroviral RNA in the samples was detected by a commercial RT-PCR kit (Enterovirus Consensus Kit, Argene, France). Sixty-one (92.4%) of 66 samples from patients with suspected viral CNS infection were found to be negative for enterovirus both with RT-PCR and shell vial cell culture methods. Three samples (4.5%) were positive by shell vial culture method. In one CSF sample that was culture positive, RT-PCR was also positive. However, the remaining two culture positive samples yielded negative result by RT-PCR. Intermediate results with RT-PCR were obtained in two samples (3%) that were identified as negative by cell culture. Two of the three positive samples in cell culture were identified as echovirus, however, the remaining sample could not be identified due to small sample amount. As a result, the commercial assay was found non-practical and labor intensive, giving indeterminant results in some cases and missing two culture positive samples. Since it didn't have an advantage over the cell culture method used, it was found inappropriate for routine diagnosis in our laboratory. On the other hand, it has been known that nucleic acid amplification tests (NAT) have markedly improved the diagnosis of enterovirus infections by increasing the sensitivity compared with cell culture methods. An alternative NAT method should be evaluated in parallel with cell culture method especially in CSF samples of children with suspected viral central nervous system infections.
The management of an adenovirus epidemic in a neonatal intensive care unit [Bir yenidogan yogun baki{dotless}m biriminde adenovirus salgi{dotless}n yönetimi]
(2011) Bilgin B.S.; Yalaz M.; Köroglu Ö.A.; Akisu M.; Çiçek C.; Büke Ç.; Kültürsay N.
Aim: Epidemic conjunctivitis caused by adenovirus frequently spreads by management contact during ophtalmologic examination. This report iswritten to share our experience on an adenovirus epidemic in our neonatal intensive care unit. Material and Method: The detailed medical and infection control committee records of the newborns and personnel together cared in Ege University neonatal intensive care unit in the period between 09.14.2009-10.17.2009 are used in this report. Conjunctival, nasopharyngeal and stool samples were collected from 15 patients, who were living in service (9 girls, 6 boys), and from 25 personnel. Weekly samples were collected from infants during the epidemic. Management fluorescence antibody test and "shell vial" cell culture method were conducted synchronously to all samples. Adenovirus conjunctivitis was diagnosed according to clinical evidence and/or detection of adenovirus in management fluorescence antibody test and cell cultures. During analysis, SPSS 13,0 statistics programme was used. Results: Severe conjunctivitis occurred within three days of fundus examination for screening of retinopathy of prematurity in five newborns who were examined in the same day and in the ophtalmologist who had examined the infants and in two newborns who were not examined. Ten of 15 infants who were cared at the neonatal intensive care unit during this epidemic had conjunctivitis, five of whom had also gastroenteritis. Five nurses had conjunctivitis and had a leave for therapy and isolation. In one of them AV was detected with direct fluorescence antibody test and also in cell cultures. Adenovirus antigen was detected in three conjunctival swabs and one tracheal aspirate of symptomatic infants. The diagnosis was confirmed with tissue cultures. In addition to standard precautions droplet precautions were also taken with the recommendation of the Infection Control Committee. Conclusions: The meticulous infection control measures, closing the unit to new admissions, detailed cleaning and decontamination of neonatal intensive care unit prevented the epidemic to spread further and limited the duration of the epidemic. No further second epidemics occurred and all patients recovered.
Molecular typing of adenoviruses isolated from clinical specimens by pcr and dna sequencing methods [Klinik ömeiklerden izole edilen adenoviruslarin pcr ve dna dizi analizi yöntemiyle tiplendirilmesi]
(2012) Çiçek C.; Şanlidag T.; Akçau S.; Sayan M.; Yalaz M.; Metin D.Y.
Adenoviruses are responsible for a broad spectrum of diseases, including upper and lower respiratory tract infections (UI^TIs and LRTIs, respectively), conjunctivitis, gastroenteritis, and hemorrhagic cystitis. The aim of this study was to determine the adenovirus (AdV) types isolated from clinical specimens by polymerase chain reaction (PCR) and DNA sequencing methods. A total of 22 AdV strains isolated between January 1 2011 to May 31 2011, from various samples (295 nasopharyngeal swabs, 42 conjunctival swabs, 13 stool) sent to our routine virology laboratory were included in the study. Of the 22 patients whose samples yielded adenovirus positivity, 8 were adult (4 were male; median age: 32.5 years) and 14 (7 were male; median age: 1 year) were children. Those specimens (14 nasopharyngeal swabs, 7 conjunctival swabs, 1 stool) were obtained from patients with URTIs (n= 6), LRTIs (n= 8), conjunctivitis (n= 7) and gastroenteritis (n= 1). For the isolation and identification of adenoviruses, rapid (shell vial) cell culture and direct immunofluorescence antibody methods were used, respectively. Molecular typing of adenoviruses were performed by PCR and sequencing of a partial region (hipervariable region 1 -6) of the hexon gene. PCR primers (Adhex FI, Adhex R1) used for DNA amplification were from those described by Lu and Erdman, previously. If insufficient DNA was amplified from the first reaction for sequencing, a nested PCR was performed using Adhex F2 and Adhex R2 primers. Sequencing was performed using the amplification primers and Sequence Reagent Mix-DYEnamic ET Terminator Cycle Sequencing Kit (Amersham Pharmacia Biotech Inc, USA) on ABI PRISM 310 Genetic Analyzer (Applied Biosystems, USA). Obtained adenovirus sequences were typed by BLAST analysis and three AdV types namely type 3, 4, and 8 were identified. In our study, AdV type 3 was detected in a gastroenteritis case and six cases with URTIs and LRTIs (n= 7, 31.8%). AdV type 8 was identified as the cause of conjunctivitis in seven patients and of URTIs and LRTIs in five patients (n= 12, 54.5%). AdV type 4 was found to be associated with URTI in one, and LRTIs in two patients (n= 3; 13.7%). Our data indicated that AdV type 8 was the most prevalent type in patients with conjunctivitis and URTIs, while AdV type 3 was the most prevalent type in patients with LRTI. BLAST analysis was thought to be useful for the molecular typing of adenoviruses. In conclusion, advanced studies with large number of specimens are necessary to achive a reliable, detailed national adenovirus database.
Percutaneous computed-tomography-guided biopsy of the spine: 229 procedures
(2006) Sucu H.K.; Çiçek C.; Rezanko T.; Bezircioglu H.; Erşahin Y.; Tunakan M.; Minoglu M.
Objectives: Percutaneous biopsy of the spine is an effective and well-evaluated procedure. Only very few series containing more than a hundred patients have been reported so far and there is no agreement about the factors affecting the diagnostic rate. We aimed to find out if there is any factor influencing the success rate of the spinal biopsy using our biopsy series. Methods: Two hundred and twenty-nine procedures were performed in 201 patients between November 2001 and August 2005. All procedures were performed under computed tomography guidance. The side was chosen according to the extension of the lesion. When the lesion was in the midline or extended to both sides, we preferred to obtain biopsy from the right side. The puncture point and the needle trajectory were planned on both lateral computed tomography scout scan and axial scans. Results: We found that the diagnostic rate was not affected by the variables such as age, gender, type and diameter of the biopsy needle, diagnosis as well as lesion localization and level. The success rate of the repeat biopsies was considerably lower than the first procedures. Conclusions: The diagnostic rate is not affected by any of the variables but the approach, chosen can vary with the level, localization, and lesion characteristics. © 2006 Elsevier SAS. All rights reserved.
Prevalence and seasonal distribution of respiratory viruses in patients with acute respiratory tract infections, 2002-2014 [Akut Solunum Yolu Enfeksiyonu Olan Hastalarda Solunum Viruslarinin Prevalansi ve Mevsimsel Da?ilimi, 2002-2014]
(Ankara Microbiology Society, 2015) Çiçek C.; Arslan A.; Karakuş H.S.; Yalaz M.; Saz E.U.; Pullukçu H.; Çok G.
The aim of this study was to investigate the prevalence and seasonal distribution of respiratory viruses in pediatric and adult outpatients and inpatients who were admitted to hospital with the symptoms of upper and lower respiratory tract infections, during a 12-year period. A total of 5102 clinical samples (4372 nasopharyngeal swabs, 316 bronchoalveolar lavages, 219 transtracheal aspirates, 163 nasopharyngeal aspirates, 20 sputum, 10 nasal swabs) examined in our laboratory between January 1st 2002 and July 17th 2014, were evaluated retrospectively. Of the specimens, 1107 (21.7%) were obtained from outpatients and 3995 (78.3%) from hospitalized patients. Of the patients, 2851 (55.9%) were male and 2251 (44.1%) were female, while 1233 (24.2%) were adults and 3869 (75.8%) were children (age range: 1 day - 93 years; median: 3 years). Respiratory samples were investigated for the presence of respiratory syncytial virus (RSV), influenza virus type A and B (INF-A, INF-B), adenovirus (AdV), parainfluenza viruses (PIV types 1-4), human rhinoviruses (HRV), human coronaviruses (HCoV), human metapneumovirus (HMPV) and human bocavirus (HBoV). All specimens were tested by both direct immunofluorescence antibody (DFA) and shell vial cell culture (SVCC) methods. In DFA assay the samples were initially screened by fluorescent-labeled polyclonal antibodies, and the positive ones were typed by using monoclonal antibodies (Light Diagnostics, Merck Millipore, USA). In SVCC, HEp-2, MDCK, A-549 and Vero cell lines were used for the isolation of viruses. In addition to these methods, real-time multiplex PCR methods (RealAccurate®, Respiratory RT PCR, PathoFinder, Netherlands and Seeplex® RV15 ACE Detection, Seegene, South Korea) were used for the detection of respiratory viruses in samples (n= 2104) obtained from 2007 to 2014. Respiratory viruses were detected in a total of 1705 (33.4%) patients, of them 967 (19%) were male and 738 (14.4%) were female. Three hundred and eighteen (18.6%) of the 1705 patients were infected with multiple respiratory viruses. The most frequently observed co-infections were RSV+INF-A (40/318; 12.6%), and RSV+PIV (33/318; 10.4%). The rate of positivity for the respiratory viruses in pediatric and adult groups were 35.4% (1369/3869) and 27.3% (336/1233), respectively (p< 0.000). The most frequently detected virus in pediatric group was RSV (336/1369;24.5%), followed by influenza viruses (314/1369;22.9%), PIV (197/1369;14.4%), HRV (118/1369;8.6%), AdV (75/1369;5.5%) and the others (49/1369;3.6%). On the other hand the most frequently detected virus in adult group was influenza viruses (181/336; 53.8%) followed by AdV (37/336; 11%), RSV (24/336; 7.1%), PIV (24/336; 7.1%), HRV (23/336; 6.8%) and the others (9/336; 2.7%). The rate of multiple virus infections in pediatric and adult groups were 7.2% (280/3869) and 3% (38/1233), respectively. Most of the coinfections (280/318; 88%) were detected in children. Respiratory viruses were detected positive in 40.2% (445/1107) of outpatients, and in 31.5% (1260/3995) of inpatients (p< 0.000). The most frequent viruses detected in pediatric outpatients and inpatients were HRV and RSV, respectively, while influenza viruses were the first in line among both adult outpatients and inpatients. During the study period, a PIV-3 outbreak (n= 96) have emerged between December 2004-April 2005, and an influenza A (H1N1)pdm09 outbreak (n= 207) between November 2009-January 2010. When the seasonal distribution was considered, the isolation rates of 1705 respiratory viruses in winter, spring, summer and autumn were 44.4%, 27%, 8.3% and 20.3%, respectively. RSV was most frequently detected from December to March, influenza viruses from November to March, HRV from December to June, and mixed infections from January to February. In conclusion, the data of our study obtained in about 12-year period indicated that the prevalence of respiratory viruses in acute respiratory infections is 33.4%, and they typically active during the months of winter and early spring in our region.
Relationship between condom usage and lower urinary tract symptoms in sexually active young men [Seksüel aktif genç erkeklerde kondom kullani{dotless}mi{dotless}ni{dotless}n alt üriner system semptomlari{dotless} ile ilişkisi]
(2011) Çoban G.; Gürer E.; Kalemci S.; Çiçek C.; Aydemir S.; Çikili N.
Objective: The effect of condom usage on the presence and intensity of the lower urinary tract symptoms (LUTS) in sexually active young men was evaluated. Materials and methods: Seventy patients (35 were using condoms and the other 35 were not), who applied to our outpatient clinic with LUTS between December 2009-May 2009, who were sexually active, aged 18-45 years, and complying with study criteria were included in the study. Patients have been evaluated with anamnesis, physical examination, International Prostate Symptom Score (IPSS) questionnaire, 2-cup test, uroflowmetry, postvoid residual volume, and transrectal ultrasonography. Results: Mean age of the patients was 31.79±7.23 years, sexually active time was 123.29±74.03 months, and symptom duration was 31.23±32.29 months. Although condom usage has no significant effect on IPSS, urinating with strain, dysuria, frequent urination, nocturia; feeling of pain or discomfort during ejaculation was more common among condom users (59.6% vs. 40.4%, p=0.041). Mean uro-flowmetry Qmax value was 20.80±1.95 and 15.83±2.56, in non-condom users and condom users, respectively (p<0.001). Five non-condom users (14.3%) and 10 condom users (28.6%) showed positivity in post-massage urine culture (p=0.244). Two non-condom users and 8 condom users showed coagulase negative staphylococcus in urine culture (p<0.01). Twenty-five condom users (57.1%) and 9 non-condom users (25.7%) showed positivity in post-massage chlamydia cell culture (p=0.015). Conclusion: Condom usage is a predisposing factor for LUTS. Increment of gram-positive bacteria and Chlamydia trachomatis positivity in post-massage prostate secretion culture shows analogy with the pain during ejaculation in these patients and gives preliminary data on the retrograde flow hypothesis.
Risk factors for infuenza virus related severe lower respiratory tract infection in children
(Lippincott Williams and Wilkins, 2019) Eşki A.; Öztürk G.K.; Gülen F.; Çiçek C.; Demir E.
Background: Influenza virus is one of the most common respiratory pathogens for all age groups and may cause seasonal outbreaks. Our aim was to identify risk groups and factors associated with severe clinical course including mortality in children with influenza-related lower respiratory tract infection (LRTI). Methods: We conducted a retrospective study in children hospitalized with influenza virus LRTI from 2008 to 2018. Data on demographic features, influenza type, viral coinfection, primary and secondary bacterial infections (SBIs), time of onset of antiviral treatment, comorbidities, hospitalization length, pediatric intensive care unit admission/invasive mechanical ventilation (IMV) need and mortality were collected from medical records. Results: There were 280 patients hospitalized with LRTI and median hospitalization length was 9 days. Congenital heart disease, neuromuscular disease, SBIs and late-onset antiviral treatment were independent risk factors for prolonged hospital stay (P < 0.05). Pediatric intensive care unit admission was present in 20.4% (57) of the patients and 17.1% (48) of all patients required IMV. SBIs, lymphopenia, neutrophilia, immunosuppression and human bocavirus coinfection were independent risk factors for IMV support (P < 0.05). Eighteen patients died and immunosuppression, lymphopenia and SBIs were independent risk factors for mortality (P < 0.05). Conclusions: Presence of comorbidity, SBIs, neutrophilia and lymphopenia at admission identified as risk factors for severe influenza infections including need for IMV and death. Although several studies showed that antiviral treatment reduce hospitalization, complications and mortality, there is a lack of prospective trials and patients for antiviral therapy should be carefully chosen by the clinician. Copyright © 2019 Wolters Kluwer Health, Inc. All rights reserved.
Simultaneous detection of respiratory viruses and influenza a virus subtypes using multiplex PCR [Solunum Viruslari ve ¯nfluenza A Virus Alt Tiplerinin Multipleks PCR Yöntemi ile Ayni Anda Saptanmasi]
(Ankara Microbiology Society, 2014) Çiçek C.; Bayram N.; Anil M.; Gülen F.; Pullukçu H.; Saz E.U.; Telli C.; Çok G.
This study was conducted to investigate the respiratory viruses and subtyping of influenza A virus when positive by multiplex PCR in patients with flu-like symptoms, after the pandemic caused by influenza A (H1N1)pdm09. Nasopharyngeal swab samples collected from 700 patients (313 female, 387 male; age range: 24 days-94 yrs, median age: 1 yr) between December 2010 - January 2013 with flu-like symptoms including fever, headache, sore throat, rhinitis, cough, myalgia as defined by the World Health Organization were included in the study. Nucleic acid extractions (Viral DNA/RNA Extraction Kit, iNtRON, South Korea) and cDNA synthesis (RevertAid First Strand cDNA Synthesis Kits, Fermentas, USA) were performed according to the manufacturer's protocol. Multiplex amplification of nucleic acids was performed using DPO (dual priming oligonucleotide) primers and RV5 ACE Screening Kit (Seegene, South Korea) in terms of the presence of influenza A (INF-A) virus, influenza B (INF-B) virus, respiratory syncytial virus (RSV), and the other respiratory viruses. PCR products were detected by automated polyacrylamide gel electrophoresis using Screen Tape multiple detection system. Specimens which were positive for viral nucleic acids have been further studied by using specific DPO primers, FluA ACE Subtyping and RV15 Screening (Seegene, South Korea) kits. Four INF-A virus subtypes [human HI (hH1), human H3 (hH3), swine HI (sHI), avian H5 (aH5)] and 11 other respiratory viruses [Adenovirus, parainfluenza virus (PIV) types 1-4, human bocavirus (HBoV), human metapneumovirus (HMPV), rhinovirus types A and B, human coronaviruses (HCoV) OC43, 229E/NL63] were investigated with those tests. In the study, 53.6% (375/700) of the patients were found to be infected with at least one virus and multiple respiratory virus infections were detected in 15.7% (59/375) of the positive cases, which were mostly (49/59, 83%) in pediatric patients. RSV and rhinovirus coinfections were the most prevalent (18/29, 62.7%) dual infections. The distribution of 436 respiratory viruses identified from 375 patients were as follows; 189 (43.3%) RSV, 93 (21.4%) rhinovirus, 86 (19.8%) INF-A, seven (1.6%) INF-B, 22 (5%) PIV types 1-3, 14 (3.2%) HMPV, 11 (2.5%) HCoV, nine (2%) HBoV, and five (1.2%) adenovirus. Fifty-five (64%) out of 86 INF-A viruses were subtyped as hH3, 24 (27.9%) were sHI and seven (8.1%) were hHI. Avian H5 was not detected in any samples. The overall prevalence rates of INF-A, INF-B, RSV and other respiratory viruses were 12%, 1 %, 27%, and 14.6%, respectively. RSV was the most prevalent respiratory agent in pediatric (161/313, 51 %) cases, while INF-A virus in adult (24/62, 38.7%) patients. Influenza viruses were detected as responsible pathogens in 13.3% (93/700) of the patients with flu-like symptoms. Among the cases, a 1-month-old baby was infected with three virus strains (INF-A hHI+INF-A sHI +HCoV OC43) and a 82-year-old patient was infected with two INF-A virus subtypes (hH3 + sH1). INF-A viruses were mostly detected (79/86) in winter period, from December to March. INF-A virus sH1, was the most prevalent subtype in flu cases till February 2011 (22/86), after replaced by INF-A virus hH3. Beginning from February 2012, a significant increase observed in the cases infected with INF-A virus subtype hH3 (39/86). In conclusion, the identification and surveillance of influenza virus types and subtypes circulating in populations have importance both for epidemiological data and selection of vaccine strains.
Specialist training in basic sciences in University Hospitals: Specialist resident perspective [Üniversite hastanelerinde temel bilimler alaninda uzmanlik egitimi: Tipta uzmanlik ögrencisi bakiş açisi ile]
(2005) Çiçek C.; Terzi C.; Solak A.; Arsu G.; Batu J.; Vatansever K.; Aslan Ö.
In this study, a questionnaire survey including 56 questions and 154 variables has been undertaken for 74 specialist residents (SpRs) (39 from Dokuz Eylül and 35 from Ege Universities) who have been training in the two university hospitals of Izmir, for the detection of the structure of the actual training programmes, trainer profiles and technical institute equipments in the departments of Basic Sciences, Medical Pathology and Pharmacology. By using this survey, SpRs' demographic informations, training programmes, theoretical and skill activites, educational atmospheres, the trainers' profiles, assessments, audits and professionel developments have been questionned. The rate of response was 71%, and educational programmes, training and technical equipments, efficacy and number of the trainers were found satisfactory in each one of the universities. It has been detected that, the SpRs were permanently assessed and they were able to get training knowledges, skills and attitutes and attend investigation activities during their educational process. This survey study indicated that the training programmes, institute equipments and trainer profiles of the universities were competent for the SpRs'.
Ten year retrospective evaluation of the seasonal distribution of agent viruses in childhood respiratory tract infections [Çocukluk çagi{dotless} solunum yolu enfeksiyonlari{dotless}nda etken virüslerin mevsimsel dagi{dotless}li{dotless}mi{dotless}ni{dotless}n 10 yi{dotless}lli{dotless}k geriye dönük degerlendirmesi]
(Galenos, 2014) Gülen F.; Yildiz B.; Çiçek C.; Demir E.; Tanaç R.
Aim: Infections caused by respiratory viruses sometimes occur as epidemias or pandemias and are an important public health problem in the whole world. These viral agents may lead to severe respiratory diseases especially in young children and in the elderly. The aim of this study was to determine the seasonal distribution of agent viruses in childhood respiratory infections in our region. Material and Methods: In this study, nasopharyngeal swab sample was obtained from 1 326 patients who presented to Ege University, Medical Faculty Children's Hospital between 2002 and 2012 and who were thought to have respiratory tract infection. Influenza virus type A and B, respiratory syncytial virus, adenovirus and parainfluenza virus type 1-3 were investigated using shell-vial cell culture method and direct fluorescent antibody test and/or multiplex PCR test. Parainfluenza virus type 4, human metapneumovirus, rhinovirus, coronavirus, human bocavirus were investigated using multiplex PCR test. The seasonal distributions of the viruses were determined according to the results obtained from Ege University Medical Faculty, Department of Medical Microbiology Clinical Virology Laboratory. Approval was obtained from the ethics committee (Ege University Clinical Researches Ethics Committee, 12.02.2013, number: 13-1/46). Results: The majority of the patients who presented were outpatients (n:888, 67%) and the remainder were hospitalized patients (33%, n:438). Respiratory viruses were found in 503 of the nasopharyngeal swab samples (38%). Parainfluenza and respiratory syncytial virus were found most frequently in december-february (58% and 59%, respectively, influenza viruses were found most frequently in november-december (72%) and adenoviruses were found most frequently in may-september (56%). Conclusion: Although only supportive therapies are administered generally in viral infections, viral investigations are important in terms of determining the measures to be taken by determining the causes as well as in terms of establishing a general database. Another benefit of this study would be strengthening clinical approach to patients and decreasing unnecessary antibiotic use. © 2014 by Turkish Pediatric Association.
Updates in the clinical virologic diagnosis of viral respiratory tract infections [Viral solunum yolu enfeksi·yonlarinin kli·ni·k vi·roloji·k tanisina güncel yaklaşim]
(2003) Çiçek C.; Bilgiç A.
Respiratory viruses (respiratory syncytial virus, influenza virus type A and B, parainfluenza virus and adenovirus) can cause a wide variety of human disease. Viral respiratory diseases in adults and children cause significant morbidity and mortality. Proper and rapid diagnosis of these etiological agents is necessary for the appropriate use of effective antiviral drugs and to decrease unnecessary antibiotic therapy. Many virology laboratories detect respiratory viruses by inoculating conventional cell culture tubes with respiratory samples and then examining for cytopathic effect or hemadsorption. However, this procedure can require many days or even weeks for viral detection and identification, providing culture results to clinicians in a period of time that may not be clinically useful. Laboratory diagnosis of respiratory virus infections has become easier and more rapid with the use of immunofluorescent antibody tests, shell vial culture methods, and molecular biological assays. In this review, article, the specificities and sensitivities of these assays were compared on the basis of recent studies, and the favorable ones for the routine diagnosis were updated.

Yazar "Çiçek C." seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri