Uygun D.A.Akduman B.Uygun M.Akgöl S.Denizli A.2019-10-262019-10-2620120273-2289https://doi.org/10.1007/s12010-012-9707-7https://hdl.handle.net/11454/18709Supermacroporous poly(2-hydroxyethyl methacrylate) [poly(HEMA)] monolithic cryogel was prepared by radical cryocopolymerization of HEMA with N, N'-methylene bisacrylamide as crosslinker. Reactive Green 5 dye was immobilized to the cryogel with nucleophilic substitution reaction, and this dye attached cryogel column was used for affinity purification of papain from Carica papaya latex. Reactive Green 5-immobilized poly (HEMA) cryogel was characterized by swelling studies, Fourier transform infrared spectroscopy, scanning electron microscopy, and energy dispersive X-ray analysis. Maximum papain adsorption capacity was found to be 68.5 mg/g polymer while nonspecific papain adsorption onto plain cryogel was negligible (3.07 mg/g polymer). Papain from C. papaya was purified 42-fold in single step with dye attached cryogel, and purity of papain was shown by silver-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis. © Springer Science+Business Media, LLC 2012.en10.1007/s12010-012-9707-7info:eu-repo/semantics/closedAccessCarica papayaCryogelKeywords Dye ligandPapainReactive green 5Article167355256322573009Q2