Celiker, TugbaGhorbanizamani, FaezehMoulahoum, HichemGuler Celik, EmineTok, KeremZihnioglu, FigenCicek, Candan2023-01-122023-01-1220220026-36721436-5073https://doi.org/10.1007/s00604-022-05244-2https://hdl.handle.net/11454/76068The construction of a rapid and easy immunofluorescence bioassay for SARS-CoV-2 detection is described. We report for the first time a novel one-pot synthetic approach for simultaneous photoinduced step-growth polymerization of pyrene (Py) and ring-opening polymerization of epsilon-caprolactone (PCL) to produce a graft fluorescent copolymer PPy-g-PCL that was conjugated to SARS-CoV-2-specific antibodies using EDC/NHS chemistry. The synthesis steps and conjugation products were fully characterized using standard spectral analysis. Next, the PPy-g-PCL was used for the construction of a dot-blot assay which was calibrated for applications to human nasopharyngeal samples. The analytical features of the proposed sensor showed a detection range of 6.03-8.7 LOG viral copy mL(-1) (Ct Scores: 8-25), the limit of detection (LOD), and quantification (LOQ) of 1.84 and 6.16 LOG viral copy mL(-1), respectively. The repeatability and reproducibility of the platform had a coefficient of variation (CV) ranging between 1.2 and 5.9%. The fluorescence-based dot-blot assay was tested with human samples. Significant differences were observed between the fluorescence intensity of the negative and positive samples, with an overall correct response of 93.33%. The assay demonstrated a high correlation with RT-PCR data. This strategy opens new insights into simplified synthesis procedures of the reporter molecules and their high potential sensing and diagnosis applications.en10.1007/s00604-022-05244-2info:eu-repo/semantics/openAccessPhotopolymerizationStep-growth polymerizationRing-opening polymerizationGraft copolymerFluorescent biosensorSARS-CoV-2Lateral Flow AssayPolymersomesDiagnosisArticle1895WOS:0007883691000032-s2.0-8512891670335474492Q1Q1