Ozcinar, OzgeTag, OzgurYusufoglu, HasanKivcak, BijenBedir, Erdal2019-10-272019-10-2720180031-9422https://doi.org/10.1016/j.phytochem.2018.04.002https://hdl.handle.net/11454/30190Biotransformation of steroidal ruscogenins (neoruscogenin and ruscogenin) was carried out with Cunninghamella blakesleeana NRRL 1369 and endophytic fungus Neosartorya hiratsukae yielding mainly P450 monooxygenase products together with a glycosylated compound. Fermentation of ruscogenins (75:25, neoruscogenin-ruscogenin mixture) with C. blakesleeana yielded 8 previously undescribed hydroxylated compounds. Furthermore, microbial transformation of neoruscogenin by endophytic fungus N. hiratsukae afforded three previously undescribed neoruscogenin derivatives. While hydroxylation at C-7, C-12, C-14, C-21 with further oxidation at C-l and C-7 were observed with C. blakesleeana, N. hiratsukae biotransformation provided C-7 and C-12 hydroxylated compounds along with C-12 oxidized and C-1(O) glycosylated derivatives. The structures of the metabolites were elucidated by 1-D (H-1, C-13 and DEPT135) and 2-D NMR (COSY, HMBC, HMQC, NOESY, ROESY) as well as HR-MS analyses. (C) 2018 Elsevier Ltd. All rights reserved.en10.1016/j.phytochem.2018.04.002info:eu-repo/semantics/closedAccessMicrobial biotransformationRuscusRuscogeninsNeoruscogeninCunninghamella blakesleeanaNeosartorya hiratsukaeArticle15219WOS:00043738330000129689318Q1Q1