Poslu, Ayse HalicBalaban, RumeysaNalbantsoy, AyseErtik, OnurCecener, GulsahKoz, OmerKoz, Gamze2025-05-082025-05-082024Poslu, A. H., Balaban, R., Nalbantsoy, A., Ertik, O., Çeçener, G., Koz, Ö., & Koz, G. (2024). Discovery of a Uracil‐Derived small organic ligand with cytotoxic effect on human PC‐3 cell lines via apoptosis. ChemistrySelect (Weinheim), 9(36), n/a.23656549https://doi.org/10.1002/slct.202403713https://hdl.handle.net/11454/117223A series of novel 6-amino-5-salicylidene uracils (1-19) were efficiently synthesized from the condensation reaction of 5,6-diamino-1,3-dimethyluracil with substituted salicylaldehydes. The structural characterization of the compounds was performed with spectroscopic methods and elemental analysis. All compounds were evaluated for their in vitro cytotoxic activity against PC-3 (human prostate adenocarcinoma), A549 (human alveolar adenocarcinoma) and SHSY-5Y (human neuroblastoma) cancer cell lines. 3,5-di-tert-Butylsalicylaldehyde derived salicylideneuracil (6ASU-8) showed promising cytotoxic activity against PC-3 cells with an IC50 value of 1.53 +/- 1.01 mu M, compared to doxorubicin, which had an IC50 value of 3.77 +/- 1.34 mu M. 6ASU-8 induced cell cycle arrest at the G2/M phase and promoted apoptosis in PC-3 cells (p<0.05*). Molecular docking results supported the experimental data, indicating that 6ASU-8 is more effective than doxorubicin. Additionally, in silico ADME analysis revealed that 6ASU-8 possesses acceptable predictive physicochemical properties.en10.1002/slct.202403713info:eu-repo/semantics/closedAccessApoptosisCytotoxicityMolecular dockingPC-3UracilArticle93619WOS:0013187698000012-s2.0-8520472413Q3Q3