Arslan, MustafaSengel, Tulay YilmazGuler, EmineGumus, Z. PinarAldemir, EbruAkbulut, HuseyinCoskunol, HakanTimur, SunaYagci, Yusuf2019-10-272019-10-2720171759-99541759-99621759-99541759-9962https://doi.org/10.1039/c7py00420fhttps://hdl.handle.net/11454/32029A double fluorescence mu-well assay that exploits a novel conjugated polymer containing cyclodextrin (CD) as the key component is reported. For the construction of the cocaine bioassay, poly(p-phenylene) with CD units in the main-chain and poly(ethylene glycol) side chains (PPP-CD-g-PEG) was first prepared by Suzuki coupling polymerization and coated on each well as a biomimetic material. Although the poly-phenylene backbone is responsible for the fluorescence properties without an additional fluorophore, PEG and CD provide water solubility and selective complexation with cocaine, respectively. A cocaine antibody was used as a secondary recognition compound after labelling with quantum dots (QDs). Most notably, we show that the two-color fluorescence nature of the assay facilitates double measurement from the same sample and the described strategy can be adapted to various sensing systems.en10.1039/c7py00420finfo:eu-repo/semantics/closedAccessArticle82133333340WOS:000402300400012Q1