Avcibasi, UgurDewa, Mouhaman ToukourKaratay, Kadriye BusraKilcar, Ayfer YurtMuftuler, Fazilet Zumrut Biber2024-08-252024-08-2520231874-47101874-4729https://doi.org/10.2174/1874471016666230110144021https://hdl.handle.net/11454/102099Background In recent years, there has been a significant increase in studies investigating the potential use of plant-origin products in the treatment and diagnosis of different types of cancer. Methods In this study, Estragole (EST) was isolated from basil leaves via ethanolic extraction using an 80% ethanol concentration. The isolation process was performed using the High Performance Liquid Chromatography (HPLC) method. The EST isolated from the basil plant was radiolabeled with I-131 using the iodogen method. Quality control studies of the radiolabeled EST (I-131-EST) were carried out by using Thin Layer Radio Chromatography (TLRC). Next, in vitro cell, culture studies were done to investigate the bio-affinity of plant-originated EST labeled with I-131 on human medulloblastoma (DAOY) and human glioblastoma-astrocytoma (U-87 MG) cell lines. Finally, the cytotoxicity of EST was determined, and cell uptake of I-131-EST was investigated on cancer cell lines by incorporation studies. Results As a result of these studies, it has been shown that I-131-EST has a significant uptake on the brain cells. Conclusion This result is very satisfying, and it has encouraged us to do in vivo studies for the molecule in the future.en10.2174/1874471016666230110144021info:eu-repo/semantics/closedAccessBasil plantestragole (EST)iodine-131 (I-131)estragole radiolabeled with I-131 (I-131-EST)human medulloblastoma (DAOY)human glioblastoma-astrocytoma (U87-MG)Medicinal-PlantsBiodistributionLeavesI-131RadioiodinationPerspectiveMorphineArticle162140150WOS:0010155853000062-s2.0-8516126353836627786Q3Q3