Ozcan, Duygu OvaOvez, Bikem2023-01-122023-01-1220221878-81811878-8181https://doi.org/10.1016/j.bcab.2022.102508https://hdl.handle.net/11454/76427In this study, the effects of temperature, light intensity, and aeration rate on the biomass, total lipid, and the residual water-soluble fractions of Phaeodactylum tricornutum were ascertained. The maximum total lipid yield of 22.39% was attained at a n-hexane/biomass ratio of 200/1, Soxhlet extraction time of 6 h and a heating rate level of 6 at 69 degrees C. The optimum fatty acid composition with a mass fraction of 99.70% was detected at a temperature of 10 degrees C, a light intensity of 431 mu mol m-2 s-1, and an aeration rate of 3.0 L min-1. The total fatty acid consisted of a major percentage of MUFAs (46.1-56.6%), followed by SFAs (39.5-53.2%), and PUFAs (0-12.5%) pro-viding a CN index of 59.3, IV of 70 g I2/100 g FAMEs, DU of 71%, CFPP of -4.5 degrees C, and SV and HHV of 205 mg KOH g-1 and 39.5 MJ kg-1, respectively, meeting the requirements of EN 14214 and ASTM D6751. Additionally, the maximum total phenol content value of 3.53 mg Gallic acid equivalent g-1 dry weight was obtained via a Folin-Ciocalteu assay at 22 degrees C, 98 mu mol m-2s-1, and 3.0 L min-1, while, the maximum total antioxidant capacity of 16.27 mu mol Trolox equivalent an-tioxidant capacity g-1 dry weight was observed with the help of the ABTS method over a broader cultivation condition range. Hence, P. tricornutum can be considered as a potential feedstock for biofuel and value-added bioproduct production offering a novel integrated lab-scale biorafina-tion process.en10.1016/j.bcab.2022.102508info:eu-repo/semantics/closedAccessAntioxidantExtractionLipidPhaeodactylum tricornutumPhenolAnd transesterificationMicroalgaeBiodieselOilArticle45WOS:0008859439000022-s2.0-85139737706Q1N/A