Ozdemir, ElifSendemir-Urkmez, AylinYesil-Celiktas, Ozlem2019-10-272019-10-2720130896-84461872-81620896-84461872-8162https://doi.org/10.1016/j.supflu.2012.12.031https://hdl.handle.net/11454/46695The effects of fabrication techniques were investigated for preparation of a chitosan based scaffold. A two-step process was used: fabrication of hydrogel which was subsequently processed either with supercritical CO2 (SC-CO2) or lyophilization. Various pressures from subcritical to supercritical (70, 160, 250 bar), temperatures (35, 45, 55 degrees C) and durations (2-3 h) were applied in order to elicit the optimum process parameters yielding the highest porosity which were determined as 250 bar, 45 degrees C, 2 h of processing at 5 g/min CO2 flow rate yielding a porosity of 87.03% which was similar to lyophilization (88.68%) achieved at 55 degrees C for 48 h. When osteosarcoma cells possessing cellular features of osteoblasts were seeded, SC-CO2 dried scaffold proved to be a more ideal support for cell attachment owing to the presence of both nano and micropores, thereby providing a more efficient and rapid alternative for tissue engineering applications. (c) 2013 Elsevier B.V. All rights reserved.en10.1016/j.supflu.2012.12.031info:eu-repo/semantics/closedAccessTissue engineeringChitosan scaffoldSupercritical CO2 dryingPorosityOsteoblastic cellArticle75120127WOS:000315756900015N/AQ1