Muderrisoglu C.Yesil-Celiktas O.2019-10-272019-10-2720191011372X1011-372Xhttps://doi.org/10.1007/s10562-019-02745-3https://hdl.handle.net/11454/24958Abstract: Baicalein, showing stronger pharmacological activity, can be obtained by removal of the distal glucuronic acid (GluA) from baicalein 7-O-ß-D-glucuronide (baicalin). In the present study, a chemically defined reaction medium comprised of mildly acidic (pH 4.5, 37 °C) aqueous solution, was formulated for biotransformation of baicalin to baicalein using acidic Helix pomatia derived beta-glucuronidase (HP-GUS), an untested biocatalyst source. The biotransformation was carried out as a batchwise process within an optimised reaction cocktail (with 5% dimethylformamide, v/v) by a 4-h HP-GUS (250 unit/ml) incubation of baicalin (60 ppm) and resulted in a promising conversion ratio of 99% without any by-product formation. The formulated reaction system may offer a novel and efficient alternative for bioproduction of baicalein, which can be vital for pharmaceutical applications. Graphical Abstract: [Figure not available: see fulltext.]. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.en10.1007/s10562-019-02745-3info:eu-repo/semantics/closedAccessBaicaleinBaicalinBiotransformationScutellaria baicalensis Georgiß-GlucuronidaseArticleQ2