Sonmez, BurakSayin, SerkanYalcinkaya, Esra EvrimSeleci, Didem AgYildiz, Huseyin BekirDemirkol, Dilek OdaciTimur, Suna2019-10-272019-10-2720142046-20692046-2069https://doi.org/10.1039/c4ra11818ahttps://hdl.handle.net/11454/48508Here we report the synthesis, characterization and application of calixarene (Calix) modified montmorillonite (Mt) as a platform for bio-applications such as biomolecule immobilization and biosensing technologies. This modification enhanced the biomolecule immobilization capability of Mt. Initially, amino-functionalised calixarenes (Calix-NH2) were synthesized and used as a modifier. X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric analysis were performed to verify the modification of the clay minerals. For the biosensor construction, Calix-NH2 modified Mt (Calix-NH2/Mt), bovine serum albumin (BSA), glutaraldehyde (GA) and pyranose oxidase were immobilized on the surface of a glassy carbon electrode which was then referred to as a CalixNH(2)/ Mt/PyOx biosensor. After optimization of the enzyme amount and pH, analytical characteristics were investigated in detail.en10.1039/c4ra11818ainfo:eu-repo/semantics/closedAccessArticle41086289562902WOS:000345701500012Q2Q1