Kayran, Yasin UgurOzkan-Ariksoysal, DilsatTezcanli, BurcinKosova, BuketOzsoz, Mehmet2019-10-272019-10-2720131040-03971521-41091040-03971521-4109https://doi.org/10.1002/elan.201300386https://hdl.handle.net/11454/48888In this study, for the first time a model electrochemical kit was constructed for the detection of a functional polymorphism in catechol-O-methyl transferase (COMT) gene which is important for diagnosis of neuropsychiatric disorders as Alzheimer disease. The disposable pencil graphite electrode (PGE) is designed as a kit and the probe DNA covered PGE can detect single nucleotide polymorphisms (SNPs) from real samples based on the guanine oxidation signal even after 5 months of kit preparation (150 days durability).The detection limit (S/N=3) of the biosensor was calculated as 1.18pmol of synthetic target sequence and 6.09x10(5) molecules of real samples in 30min detection time.en10.1002/elan.201300386info:eu-repo/semantics/closedAccessElectrochemical DNA biosensorPoint-of-care testReady-to-use kit designSingle nucleotide polymorphismCatechol-O-methyl transferase (COMT) Val108158Met polymorphismArticle251226682676WOS:000328326100013Q2Q2