Kavruk, EbruKarabey, BurcinTemal, Hulya YilmazTuna, Elif Esin Hames2020-12-012020-12-0120191018-46191610-23041018-46191610-2304https://hdl.handle.net/11454/63630It has been known that most of the microorganisms that constitute microbial diversity in marine environments have symbiotic relationship with sponges. Metagenomics methods in which the genetic material of the microorganism is used due to the inadequacy of conventional cultivation techniques exposing this diversity took an important place in the recent years. in this study, microbial diversity of four sponge samples; Agelas oroides, Ciocalypta carballoi, Petrosia ficiformis and Sarcotragus foetidus collected from Mediterranean Sea has been analyzed by metagenomics approaches. By using PCR-DGGE (Denaturing Gradient Gel Electrophoresis), a total of 34 16S rDNA-V3 and 10 of ITS1 bands were isolated, sequenced and BLAST analyses were performed to identify the nearest sequence match. According to the results, the bacterial diversity of the four sponges was higher than the fungal diversity. Most common Proteobacteria phylum members were identified in all four sponge samples. This is the first report on the identification of uncultured Roseospirillum sp. from A. oroides, lichen-forming terrestrial uncultured Trichopezizella relicina from P. ficiformis, terrestrial uncultured Cellulomonas sp. and Actinobacterium from C. carballoi. Fungal diversity was determined only in A. oroides and P. ficiformis. Fungal sequences obtained from A. oroides, were found highly similar to uncultured fungal sequences from marine sediment.eninfo:eu-repo/semantics/closedAccessMicrobial DiversitySponge MicrobiomeDenaturing Gradient Gel ElectrophoresisMetagenomicsArticle2812A1001110020WOS:000501936600043Q4