Jadid, Mahdieh Farhoudi SefidanShademan, BehrouzChavoshi, RezaSeyyedsani, NasrinAghaei, ElnazTaheri, ElhamSabet, Mehrdad Nasrollahzadeh2021-05-032021-05-0320211520-40811522-7278https://doi.org/10.1002/tox.23103https://hdl.handle.net/11454/69728Many chemotherapeutic regimens have been investigated for advanced unresectable and metastatic pancreatic cancer (PC), but with only minimal improvement in survival and prognosis. Here, we investigated anti-cancer function of free and nano-encapsulated hydroxytyrosol (Hyd) and curcumin (Cur), and its combinations (Hyd-Cur) on PANC-1 cell line. The poly lactide-co-glycolide-co-polyacrylic acid (PLGA-co-PAA) nano-encapsulated Hyd and Cur were synthesized, and MTT assay was performed to evaluate cytotoxic effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur. Effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur were evaluated on viability, migration, morphological alterations, colony formation, and apoptosis on PANC-1 cells. We observed that free and nano-encapsulated Hyd, Cur, and Hyd-Cur significantly increased apoptosis rates as well as significantly decreased viability, migration, and colony formation in PANC-1 cells. According to our results, Hyd-Cur combination and nano-encapsulation therapy exerts more profound apoptotic and anti-proliferative effects on PANC-1 cells than free Hyd or Hyd monotherapy.en10.1002/tox.23103info:eu-repo/semantics/closedAccessapoptosiscurcuminhydroxytyrosolpancreatic cancerPLGA-PAA copolymerArticle36610431051WOS:0006120517000012-s2.0-8509996067533496383Q2N/A