Kalburcu, TuldenTabak, AhmetOzturk, NevraTuzmen, NalanAkgol, SinanCaglar, BulentDenizli, Adil2019-10-272019-10-2720150022-28601872-80140022-28601872-8014https://doi.org/10.1016/j.molstruc.2014.11.055https://hdl.handle.net/11454/40454This study deals with developing bentonite-tryptophan (Bent-Trp) microcomposite affinity sorbent (38-105 gm) for lysozyme adsorption from aqueous solutions. The structural analysis results of Bent-Trp microcomposites obtained from X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermal (TG-DTG/DTA) and elemental analysis techniques clearly pointed out that the pseudo-biospecific affinity ligand L-Tryptophan was penetrated into interlayer space of the bentonite by diplacing water molecules and an elemental analysis of immobilized L-Tryptophan for nitrogen was 541.3 mu mol g(-1) bentonite. The effects of initial concentration, pH, and temperature on the adsorption efficiency of this sorbent were also studied in a batch system. The maximum amount of lysozyme adsorption from aqueous solution was determined as 365.16 mg/g at pH 10.0 and 1.25 mg/mL initial concentration of lysozyme, while the non-specific adsorption of lysozyme was very low (4.21 mg g(-1) sorbent). We concluded that Bent-Trp microcomposite affinity sorbents could be repeatedly applied for lysozyme adsorption without significant losses in the adsorption capacity. (C) 2014 The Authors. Published by Elsevier B.V.en10.1016/j.molstruc.2014.11.055info:eu-repo/semantics/closedAccessBentonite-tyrptophane microcompositeLysozymeAffinity chromatographyTG-DTG/DTAXRDArticle1083156162WOS:000348879500021Q2Q3