Cogulu, DilsahOnay, HuseyinOzdemir, YaseminAslan, Gulcin I.Özkınay, FerdaKutukculer, NecilEronat, Cemal2019-10-272019-10-2720151343-49341880-4926https://doi.org/10.2334/josnusd.57.31https://hdl.handle.net/11454/50785Streptococcus mutans is important in dental caries. Although the role of cytokines in the pathogenesis of dental caries is not clear, components of Streptococcus mutans were found to stimulate production of pro-inflammatory cytokines. We examined the associations of interleukin (IL)-1 beta, IL-1 receptor antagonist (IL-1ra), and IL-10 with dental caries. Unstimulated whole saliva and blood samples were obtained from 108 children aged 6-12 years with high caries (decayed, missing, or filled teeth [dmft/DMFT] index >4, n = 37), moderate caries (dmft/DMFT = 1-4, n = 37), or caries-free (dmft/DMFT = 0, n = 34). Streptococcus mutans level was classified as low (<10(5) colony-forming units [CFU]/mL) or high (>= 10(5) CFU/mL). Saliva and serum concentrations of IL-1 beta, IL-1ra, and IL-10 were determined by ELISA. IL-1 beta, IL-1ra and IL-10 gene polymorphisms were genotyped using PCR and restriction fragment length polymorphism analysis. The chi-square, Mann-Whitney U, one-way ANOVA, posthoc, Fisher's exact, and t tests were used in statistical analysis. Dental caries was not correlated with salivary or serum concentrations of the studied cytokines. Streptococcus mutans level positively correlated with saliva IL-1 beta concentration and inversely correlated with saliva IL-1ra concentration. There was no correlation of IL-1 beta, IL-1ra, or IL-10 gene polymorphisms with dental caries. Streptococcus mutans is important in stimulating saliva IL-1 beta and inhibiting IL-1ra. Future studies of associations between cytokines and dental caries should investigate additional cytokines and enroll a larger number of participants.en10.2334/josnusd.57.31info:eu-repo/semantics/openAccessdental cariesStreptococcus mutansIL-1 betaIL-1raIL-10Article5713136WOS:00035165790000525807906Q4