Emingil G.Han B.Gürkan A.Berdeli A.Tervahartiala T.Salo T.Pussinen P.J.Köse T.Atilla G.Sorsa T.2019-10-272019-10-2720140022-3492https://doi.org/10.1902/jop.2013.130365https://hdl.handle.net/11454/26318Background: The aim of the present study is to investigate matrix metalloproteinase (MMP)-8 and tissue inhibitor of MMP-1 (TIMP-1) gene polymorphisms in generalized aggressive periodontitis (GAgP) and to assess the effects of MMP-8 and TIMP-1 genotypes on the outcomes of non-surgical periodontal therapy. Methods: Genomic DNA was obtained from peripheral blood of 100 patients with GAgP and 167 periodontally healthy controls. MMP-8 +17 C/G, -799 C/T, -381 A/G and TIMP-1 372 T/C, *429 T/G polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism. Patients with GAgP received non-surgical periodontal therapy and were followed for 6 months. Clinical periodontal parameters and gingival crevicular fluid (GCF) samples were collected at baseline and at follow-up visits. GCF biomarkers were analyzed by immunofluorescence assay and enzyme-linked immunosorbent assay. Results: Distribution of the MMP-8-799 C/T genotypes was significantly different between the GAgP and control groups (P <0.005). TIMP-1 372 T/C and *429 T/G genotypes in males were also significantly different between study groups (P <0.004). GCF MMP-8 levels decreased until 3 months after non-surgical therapy compared with baseline in T and G alleles, as well as G and C allele carriers (P <0.0125), whereas no significant decreased was observed in non-carriers (P >0.0125). Conclusion: On the basis of the present findings, it can be suggested that MMP-8-799 C/T and TIMP-1 372 T/C, *429 T/G gene polymorphisms in males may be associated with the susceptibility to GAgP in the Turkish population.en10.1902/jop.2013.130365info:eu-repo/semantics/closedAccessAggressive periodontitisGenesGingival crevicular fluidMatrix metalloproteinase 8PolymorphismTissue inhibitor of metalloproteinase-1Article8581070108024283658Q1