Içhedef C.Teksöz S.Ünak P.Medine E.I.Ertay T.Bekiş R.2019-10-262019-10-2620121388-07641388-0764https://doi.org/10.1007/s11051-012-1077-0https://hdl.handle.net/11454/18540Magnetic nanoparticles were prepared by a reduction-precipitation method and coated with an amino silane coupling agent. Guanine (Gua) was conjugated to the magnetic nanoparticles (MNPs) using glutaraldehyde as a cross-linker. Common techniques (Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction, and vibrating electron microscopy) were used to assess the properties of the particles. Structural investigations showed that amino silane-coated MNPs had a particle size of about 40-60 nm in diameter with a spherical morphology. The guanine-conjugated MNPs were radiolabeled with 99mTc(I)-tricarbonyl core ( 99mTc(CO) 3-MNP-Gua) with a labeling yield of 72 ± 4 %. Pure radiolabeled magnetic particles were obtained by washing them with saline solution, and the radiochemical purity of 99mTc(CO) 3-MNP-Gua was 98 ± 2 % in the final solution. The biologic distribution of guanine MNPs was assessed in New Zealand rabbits using a gamma camera. In the in vivo experiment, a high level of radioactivity was observed in the lungs and liver soon after intravenous administration of 99mTc(CO) 3-MNP-Gua. © 2012 Springer Science+Business Media B.V.en10.1007/s11051-012-1077-0info:eu-repo/semantics/closedAccessGuanineImagingMagnetic nanoparticlesTechnetium tricarbonyl coreArticle148Q2