Dai, PeiJiao, FangyanYang, LuluBajinka, OusmanAbdelhalim, Khalid A.Wu, GuojunTan, Yurong2024-08-252024-08-2520232036-7481https://doi.org/10.3390/microbiolres14020044https://hdl.handle.net/11454/103178The aim of this study was to understand the molecular epidemiological characteristics and drug resistance mechanism of carbapenem-resistant Pseudomonas aeruginosa (CRPA) and to provide a theoretical basis for the prevention and treatment of CRPA infection in hospitals. A total of 34 CRPA strains were isolated, and resistance to 13 commonly used antibiotics was detected using the TDR-300B Plus VitEK-2 compact automatic bacterial identification instrument. Then, carbapenemase production was detected using the Carbe NP test. RT-qPCR was used to detect the expression of efflux pump MexA and outer membrane protein OprD, and PCR amplification and sequence analysis were used to detect class I integrons carried by drug resistance genes. Our results showed that of the 34 CRPAs, 22 were multi-drug resistant (MDR), and five were extensively drug-resistant (XDR). Sequencing analysis showed that class I integron mainly carried aminoglycosides or quinolones resistance genes. Multiple mechanisms play important roles in the formation and development of MDR or XDR.en10.3390/microbiolres14020044info:eu-repo/semantics/openAccessPseudomonas aeruginosacarbapenemdrug resistance mechanismsMDRXDRPumpArticle142627634WOS:0010145959000012-s2.0-85163730354N/AQ3