Babadostu A.Guldu O.K.Demirkol D.O.Medine E.I.Unak P.Timur S.2019-10-262019-10-2620150091-40370091-4037https://doi.org/10.1080/00914037.2014.936598https://hdl.handle.net/11454/17185The authors report an electrochemical phenol biosensor based on the immobilization of laccase (Lac) on the surface of copper capped magnetic core-shell (Fe3O4-SiO2) nanoparticles (MNPs). After synthesis, MNP surfaces were functionalized by silanization and then, modified with histidine (His) and copper, respectively. The performance of the MNP-His/Cu/Lac biosensor has been investigated at -0.05 V versus Ag/AgCl. The proposed biosensor allowed to detect catechol and phenol in the range of 0.01-0.4 mM and 0.025-0.2 mM, respectively. Finally, phenol analysis in culture medium of P. putida adapted to phenol was successfully demonstrated with appreciable recovery values. © 2015 Copyright Taylor & Francis Group, LLC.en10.1080/00914037.2014.936598info:eu-repo/semantics/closedAccessBioprocess monitoringbiosensorlaccasemagnetic nanoparticlesphenolic compoundssurface modificationArticle645260266Q2