de Kleijn, Dominique P. V.Moll, Frans L.Hellings, Willem E.Ozsarlak-Sozer, Gonende Bruin, PeterDoevendans, Pieter A.Vink, AryanCatanzariti, Louise M.Schoneveld, Arjan H.Algra, AleDaemen, Mat J.Biessen, E. A.de Jager, W.Zhang, Huomingde Vries, Jean-PaulFalk, ErlingLim, Sai K.van der Spek, Peter J.Sze, Siu KwanPasterkamp, Gerard2019-10-272019-10-2720101079-5642https://doi.org/10.1161/ATVBAHA.109.194944https://hdl.handle.net/11454/44020Objective-Atherosclerotic cardiovascular disease is a major burden to health care. Because atherosclerosis is considered a systemic disease, we hypothesized that one single atherosclerotic plaque contains ample molecular information that predicts future cardiovascular events in all vascular territories. Methods and Results-AtheroExpress is a biobank collecting atherosclerotic lesions during surgery, with a 3-year follow-up. The composite primary outcome encompasses all cardiovascular events and interventions, eg, cardiovascular death, myocardial infarction, stroke, and endovascular interventions. A proteomics search identified osteopontin as a potential plaque biomarker. Patients undergoing carotid surgery (n=574) served as the cohort in which plaque osteopontin levels were examined in relation to their outcome during follow-up and was validated in a cohort of patients undergoing femoral endarterectomy (n=151). Comparing the highest quartile of carotid plaque osteopontin levels with quartile 1 showed a hazard ratio for the primary outcome of 3.8 (95% confidence interval, 2.6-5.9). The outcome did not change after adjustment for plaque characteristics and traditional risk factors (hazard ratio, 3.5; 95% confidence interval, 2.0-5.9). The femoral validation cohort showed a hazard ratio of 3.8 (95% confidence interval 2.0 to 7.4) comparing osteopontin levels in quartile 4 with quartile 1. Conclusion-Plaque osteopontin levels in single lesions are predictive for cardiovascular events in other vascular territories. Local atherosclerotic plaques are a source of prognostic biomarkers with a high predictive value for secondary manifestations of atherosclerotic disease. (Arterioscler Thromb Vasc Biol. 2010;30:612-619.)en10.1161/ATVBAHA.109.194944info:eu-repo/semantics/openAccessarterectomyatherosclerosisbiomarkerplaqueArticle303612U486WOS:00027465870003820018935Q1Q1