Cetintas, Vildan BozokKaymaz, Burcin TezcanliAktug, HuseyinOltulu, FatihTaskiran, Dilek2019-10-272019-10-2720141107-06252241-6293https://hdl.handle.net/11454/48793Purpose: Capsaicin, an ingredient of red chili pepper, has possible tumorigenicity/genotoxicity properties. We aimed to determine the effects of capsaicin on the proliferation and gene expression profiles of acute lymphoblastic leukemia (ALL) CCRF-CEM cell line. Methods: Cell viability and IC50 dose was determined by WST cytotoxicity assay. qRT-PCR, immunohistochemical staining and western blot methods were used to determine target genes' expression levels, Apoptosis was evaluated by measuring the caspase-3 activity. Results: Capsaicin inhibited the proliferation of CCRF-CEM cells in a dose-dependent manner. Increased mRNA expressions of caspase gene family members, activated caspase-3 and decreased mRNA and protein expression of BCL-2 gene indicated apoptotic response to capsaicin. Moreover capsaicin treatment suppressed significantly the expression of the key cell signaling pathways of KRAS, AKT, GAB2, PTPN11, BRAF, INPP5D, MAPK7. Conclusion: Capsaicin induces apoptosis in CCRF-CE.M cells and this response is associated with downregulation of cell signaling pathways.eninfo:eu-repo/semantics/closedAccessacute lymphoblastic leukemiaapoptosiscapsaicincell signalingArticle191183190WOS:00033415300002724659662Q3N/A