Unal Kocabas, GokcenBlatti, Asli KisimBerdeli, AfigOzgen, Ahmet GokhanYurekli, Banu Sarer2025-04-182025-04-182024Unal Kocabas, G., Kisim Blatti, A., Berdeli, A., Ozgen, A. G., & Sarer Yurekli, B. (2024). MAPK pathway and NIS in B-CPAP human papillary thyroid carcinoma cells treated with resveratrol. Pathology, Research and Practice, 263, 155623.16180631https://doi.org/10.1016/j.prp.2024.155623https://hdl.handle.net/11454/117119Background: Resveratrol, a herbal phytoalexin, is known to have anti-tumor effects in several tumors including thyroid cancer cells. Aim: The aim of this study was to determine the effects of resveratrol on the expression of BRAF, ERK and NIS mRNA levels and protein expression in B-CPAP human thyroid papillary cancer cell line. Methods: B-CPAP cells were treated with resveratrol at concentrations of 10-100 mu M for 24-48-72 h. Cell viability was assessed by XTT Cell Proliferation Assay. BRAF, ERK and NIS mRNA levels were evaluated by rtPCR method. Protein expressions were evaluated by Western Blot method. Results: Resveratrol was found to inhibit cell proliferation in a time and dose dependent manner. The IC50 values of resveratrol were 18.7 mu M and 56.8 mu M after 48 h and 72 h respectively. Resveratrol treatment of B-CPAP cells resulted in up to 1.5-fold reduction in BRAF mRNA and up to 5.5 fold reduction in ERK mRNA levels. NIS mRNA levels showed up to 3-fold increase. Western Blot studies confirmed the rt- PCR results with a decrease in BRAF and ERK, and increase in NIS protein expressions. Conclusion: This study demonstrated that resveratrol inhibits thyroid papillary carcinoma cell proliferation and reduces poor prognostic BRAF and ERK mRNA and protein expressions, while increasing NIS mRNA and protein expression suggesting a redifferentiating effect. More studies are needed to evaluate resveratrol as a novel therapeutic agent in the treatment of papillary thyroid cancer.en10.1016/j.prp.2024.155623info:eu-repo/semantics/closedAccessBRAFERKNISResveratrolThyroid papillary cancerArticle263Nov15WOS:0013348146000012-s2.0-8520877270939405802Q2Q2