Kilcar A.Y.Biber Muftuler F.Z.Unak P.Avci C.B.Gunduz C.2019-10-272019-10-2720111084-9785https://doi.org/10.1089/cbr.2010.0860https://hdl.handle.net/11454/26959Cytotoxic and apoptotic effects of toremifene-diethylenetriamine pentaacetic acid (TOR-DTPA), formed by conjugation of TOR and DTPA, on the MCF-7 cell line were evaluated. TOR-DTPA was synthesized and qualified via gas chromatography-mass spectrometry system, thin layer chromatography, and high performance liquid chromatography methods. To screen the biological properties of TOR-DTPA at determined concentrations, our ongoing effort was to evaluate apoptotic and cytotoxic effects on the MCF-7 cell line. Trypan blue dye exclusion test, XTT, ELISA, and TUNEL assays were utilized to evaluate cytotoxicity and apoptosis. TOR-DTPA has no cytotoxic and limited apoptotic effect on the MCF-7 cell line according to the results of in vitro studies. It is concluded that the lack of obvious apoptotic and cytotoxic effects allows the already proposed ligand, TOR-DTPA, to be improved as a novel hydrophilic ligand for breast imaging. © Copyright 2011, Mary Ann Liebert, Inc.en10.1089/cbr.2010.0860info:eu-repo/semantics/closedAccessBreast tissueMCF-7 cell lineTOR-DTPAtoremifene (TOR)Article26110511121355781Q2