Unal, Didem NurEksin, EceErdem, Arzum2019-10-272019-10-2720180003-27191532-236X0003-27191532-236Xhttps://doi.org/10.1080/00032719.2017.1338714https://hdl.handle.net/11454/30958Disposable graphite pencil electrodes were used in this study for the electrochemical determination of 6-thioguanine using its interaction with a single-stranded Oligo (A)(25) composed of 25 adenine (A) bases or double-stranded Oligo (A)(25)-Oligo (T) 25, a hybrid form of Oligo (A)(25) and Oligo (T)(25) composed of 25 thymine (T) bases. The surface interaction was evaluated by monitoring the changes of the oxidation signals of 6-thioguanine and adenine. The electrochemical measurements were performed using differential pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectroscopy. The experimental conditions, including the concentration of 6-thioguanine and its period of interaction with single-stranded Oligo (A)(25) or double-stranded Oligo (A)(25)-Oligo (T)(25), were optimized by observing the changes in the 6-thioguanine and adenine oxidation currents by differential pulse voltammetry. The detection limit was determined to be 0.24 mu M across the useful linear concentration range from 1 to 4 mu M 6-thioguanine. The reported single-use graphite sensor provides a cost-effective, rapid, and sensitive detection approach for the electrochemical characterization of the interaction between DNA and 6-thioguanine.en10.1080/00032719.2017.1338714info:eu-repo/semantics/closedAccess6-thioguaninecyclic voltammetry (CV)differential pulse voltammetry (DPV)drug-DNA interactionelectrochemical impedance spectroscopy (EIS)Article5101.Feb265278WOS:000416519900021Q4