Yorganci, EmineAkyilmaz, Erol2019-10-272019-10-2720111073-1199https://doi.org/10.3109/10731199.2011.563363https://hdl.handle.net/11454/46008Alkaline phosphatase (ALP) was immobilized with cross-linking agents glutaraldehyde and cysteamine by forming a self-assembled monolayer on a screen printed gold electrode. ALP converts p-nitrophenyl phosphate to p-nitrophenol and phosphate. p-Nitrophenol loses H(broken vertical bar) ion and turns into the negatively charged compound p-nitrophenolate at medium pH. As a result, the unstable product formed is measured chronoamperometrically at an application potential of + 0.95 V. The biosensor response depends linearly on p-nitrophenyl phosphate concentration between 0.05 - 0.6 mM with a response time of 40 seconds. Detection limit of the biosensor is 0.033 mM.en10.3109/10731199.2011.563363info:eu-repo/semantics/closedAccessalkaline phosphatisebiosensorself-assembled monolayeramperometryp-nitrophenyl phosphateArticle395317323WOS:00029474030000921663400Q3