Ozden, CanAfacan, BeralAtmacaIlhan, HarikaKose, TimurEmingil, Gulnur2025-02-212025-02-212024Özden, C., Afacan, B., İlhan, H. A., Köse, T., & Emingil, G. (2024). Oral biofluid levels of activin-A and interleukin-1beta in stage III periodontitis. Clinical Oral Investigations, 29(1), 71432-6981https://doi.org/10.1007/s00784-024-06088-1https://hdl.handle.net/11454/116127Objectives Activin-A belongs to the transforming growth factor-beta superfamily and is a multifunctional cytokine that plays a role in inflammation, immune response, tissue repair and regeneration. Proinflammatory cytokine interleukin-1beta (IL-1 beta) can increase Activin-A expression in various cell types. This study aims to evaluate gingival crevicular fluid (GCF) and salivary Activin-A and IL-beta levels in stage III periodontitis. Materials and methods 23 patients with stage III periodontitis, 26 with gingivitis and 26 periodontally healthy individuals were included. Full-mouth clinical periodontal indices were recorded, unstimulated whole saliva and GCF samples were obtained, Activin-A and IL-1 beta total amounts were determined by ELISA. Statistical comparisons were performed using non-parametric tests. Receiver operating characteristics curve was used for estimating the area under the curve (AUC). Results Periodontitis group exhibited significantly lower GCF Activin-A levels but higher IL-1 beta levels than the periodontally healthy group (p < 0.05). Gingivitis group had similar GCF Activin-A and IL-1 beta levels to the periodontitis and periodontally healthy groups (p > 0.05). Salivary Activin-A and IL-1 beta concentrations were similar among study groups (p > 0.05). GCF Activin-A level showed an excellent diagnostic performance (an AUC value of 0.82 with 87% sensitivity) to discriminate periodontitis from periodontal health. ConclusionsFor the first time, this study demonstrated oral biofluid levels of Activin-A in periodontal health and diseases. Within the limits of the study, it might be suggested that diseased sites in periodontitis are associated with reduced Activin-A and increased IL-1 beta levels in GCF.Clinical relevance.Reduced GCF Activin-A levels and the accompanying increase in IL-1 beta might be associated with diseased sites in stage III periodontitis. ConclusionsFor the first time, this study demonstrated oral biofluid levels of Activin-A in periodontal health and diseases. Within the limits of the study, it might be suggested that diseased sites in periodontitis are associated with reduced Activin-A and increased IL-1 beta levels in GCF.Clinical relevance.Reduced GCF Activin-A levels and the accompanying increase in IL-1 beta might be associated with diseased sites in stage III periodontitis. ConclusionsFor the first time, this study demonstrated oral biofluid levels of Activin-A in periodontal health and diseases. Within the limits of the study, it might be suggested that diseased sites in periodontitis are associated with reduced Activin-A and increased IL-1 beta levels in GCF.Clinical relevance.Reduced GCF Activin-A levels and the accompanying increase in IL-1 beta might be associated with diseased sites in stage III periodontitis.en10.1007/s00784-024-06088-1info:eu-repo/semantics/closedAccessCytokineGingival crevicular fluidInflammationPeriodontitisSalivaArticle291111WOS:0013755562000012-s2.0-8521149852839656274Q1Q1